本文關(guān)鍵詞： 瘢痕 核心蛋白多糖 成纖維細(xì)胞 創(chuàng)傷愈合　出處：《昆明醫(yī)學(xué)院》2010年碩士論文　論文類型：學(xué)位論文

【摘要】： 一、論文核心蛋白多糖在瘢痕組織及成纖維細(xì)胞中的表達(dá)及意義 【目的】對(duì)病理性瘢痕組織及成纖維細(xì)胞中Decorin的含量及表達(dá)進(jìn)行檢測,深入探討并揭示Decorin對(duì)瘢痕的作用及機(jī)制,為臨床預(yù)防和治療瘢痕提供分子及病理學(xué)水平的理論依據(jù)。 【方法】(1)對(duì)病理性瘢痕成纖維細(xì)胞進(jìn)行體外培養(yǎng),采用光鏡、掃描電鏡觀察成纖維細(xì)胞形態(tài)、活性及凋亡等;提取細(xì)胞RNA,應(yīng)用RT-PCR對(duì)Decorin、TGF-β1的mRNA表達(dá)進(jìn)行檢測分析。(2)將病理性瘢痕組織進(jìn)行固定、包埋、切片,采用免疫組織化學(xué)染色對(duì)Decorin、TGF-β1進(jìn)行檢測。通過以上實(shí)驗(yàn)觀察及統(tǒng)計(jì)學(xué)分析,以研究Decorin對(duì)病理性瘢痕成纖維細(xì)胞生物學(xué)行為的影響及病理改變,Decorin在病理性瘢痕發(fā)生發(fā)展中的作用。 【結(jié)果】(1)光鏡下:成纖維細(xì)胞為貼壁生長,長梭形或多邊形,呈柵欄狀排列;與正常皮膚成纖維細(xì)胞相比,病理性瘢痕成纖維細(xì)胞形態(tài)不規(guī)則、體積較大,細(xì)胞排列較亂;(2)電鏡下:與正常皮膚對(duì)照組相比,瘢痕成纖維細(xì)胞線粒體增多,粗面內(nèi)質(zhì)網(wǎng)增多并腫脹、擴(kuò)張呈囊狀,胞質(zhì)內(nèi)微絲微管增多,細(xì)胞核內(nèi)常染色質(zhì)豐富,表明其合成蛋白的功能活躍;(3)RT-PCR結(jié)果顯示:瘢痕疙瘩成纖維細(xì)胞中Decorin mRNA含量較正常瘢痕或正常皮膚成纖維細(xì)胞降低;瘢痕疙瘩成纖維細(xì)胞中TGF-β1 mRNA表達(dá)較正常皮膚及瘢痕組織成纖維細(xì)胞升高;(4)免疫組化結(jié)果顯示:Decorin主要表達(dá)于正常皮膚真皮層細(xì)胞外基質(zhì)中及膠原纖維表面,與正常皮膚相比,Decorin在瘢痕組織中表達(dá)量明顯降低;而TGF-β1在瘢痕組織中表達(dá)量明顯高于正常皮膚中,具有統(tǒng)計(jì)學(xué)意義(P＜0.05)。 【結(jié)論】Decorin在病理性瘢痕組織及成纖維細(xì)胞內(nèi)含量較正常皮膚明顯減少,提示在創(chuàng)面愈合早期由于Decorin含量降低,使TGF-β1活性上調(diào),其對(duì)成纖維細(xì)胞的刺激作用也隨之增加,引起成纖維細(xì)胞的大量增生、遷移,并合成過量膠原,這可能是病理性瘢痕形成的一個(gè)重要因素。
[Abstract]:1. Expression and significance of core proteoglycan in scar tissue and fibroblasts. [objective] to detect the content and expression of Decorin in pathological scar tissue and fibroblasts, and to explore the effect and mechanism of Decorin on scar in order to provide theoretical basis for clinical prevention and treatment of scar. [methods] the fibroblasts of pathological scar were cultured in vitro. The morphology, activity and apoptosis of fibroblasts were observed by light microscope and scanning electron microscope. The mRNA expression of TGF- 尾 1 was detected by RT-PCR. The pathological scar tissue was immobilized, embedded, sectioned and detected by immunohistochemical staining, and the expression of TGF- 尾 1 was detected by immunohistochemical staining. To study the effect of Decorin on the biological behavior of fibroblasts in pathological scar and the role of Decorin in the pathogenesis and development of pathological scar. [results] under the light microscope, fibroblasts were adherent, fusiform or polygonal, arranged in palisade shape. Compared with normal skin fibroblasts, pathological scar fibroblasts were irregular in shape and larger in volume. Compared with the normal skin control group, the mitochondria of scar fibroblasts increased, the rough endoplasmic reticulum increased and swollen, dilated in cystic shape, the cytoplasmic microfilament increased, and the nucleus was rich in chromatin. The results of RT-PCR showed that the content of Decorin mRNA in keloid fibroblasts was lower than that in normal scar or normal skin fibroblasts. The expression of TGF- 尾 1 mRNA in keloid fibroblasts was higher than that in normal skin and scar tissue fibroblasts. Compared with normal skin, the expression of TGF- 尾 1 in scar tissue was significantly lower than that in normal skin, and the expression of TGF- 尾 1 in scar tissue was significantly higher than that in normal skin (P < 0.05). [conclusion] the content of Decorin in pathological scar tissue and fibroblast is significantly lower than that in normal skin. It is suggested that in the early stage of wound healing, TGF- 尾 1 activity is upregulated due to the decrease of Decorin content, and the stimulating effect of TGF- 尾 1 on fibroblast is also increased. The proliferation and migration of fibroblasts and the synthesis of excessive collagen may be an important factor in the formation of pathological scar.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R363

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