本文關(guān)鍵詞： 子宮內(nèi)膜異位癥 GFP小鼠 sp細(xì)胞　出處：《福建醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：背景：子宮內(nèi)膜異位癥的“干細(xì)胞學(xué)說”認(rèn)為子宮內(nèi)膜干細(xì)胞隨月經(jīng)逆流種植腹腔是內(nèi)異癥形成的關(guān)鍵，，但至今未有實(shí)驗(yàn)證據(jù)證明。 目的：探索小鼠子宮內(nèi)膜是否存在干細(xì)胞；利用帶有綠色熒光蛋白基因（GFP）的小鼠子宮內(nèi)膜細(xì)胞或組織建立ICR小鼠子宮內(nèi)膜異位癥模型。 方法：1.分離和收集含GFP基因小鼠的子宮內(nèi)膜細(xì)胞，經(jīng)流式分選出SP細(xì)胞。 2.將收集到的GFP內(nèi)膜細(xì)胞及組織移植于小鼠子宮漿膜及盆腔損傷處，實(shí)驗(yàn)分組:1)移植子宮內(nèi)膜細(xì)胞組2）移植子宮內(nèi)膜細(xì)胞與骨髓間充質(zhì)細(xì)胞共培養(yǎng)組3）盆腔腹膜損傷后，直接移植消化的子宮內(nèi)膜微組織組4）盆腔腹膜損傷3周后，移植消化的子宮內(nèi)膜微組織組。繼續(xù)飼養(yǎng)4-6周，熒光顯微鏡下直接觀察病灶情況，并進(jìn)行HE染色、免疫組化。 結(jié)果：1.新鮮消化的單細(xì)胞用Hoechst染色，經(jīng)流式細(xì)胞儀分選出SP細(xì)胞群占1.25-2.29%。 2.含GFP基因的新鮮子宮內(nèi)膜微小組織碎片移植于普通ICR小鼠可獲得異位生長，經(jīng)預(yù)損傷處理的小鼠較新鮮損傷移植的小鼠模型有更高的移植物生長率，分別為100%(12/12) VS37.5%(3/8)，移植生長物GFP標(biāo)記物陽性。 結(jié)論：小鼠子宮內(nèi)膜細(xì)胞中含有SP細(xì)胞；微組織移植比細(xì)胞移植易形成移植灶，預(yù)損傷可以提高子宮內(nèi)膜異體移植的成功率。
[Abstract]:Background: the stem cell theory of endometriosis suggests that endometrial stem cell implantation with menstrual countercurrent is the key to the formation of endometriosis, but there is no experimental evidence to prove it. Aim: to explore the existence of stem cells in mouse endometrium and to establish a model of endometriosis in ICR mice by using the cells or tissues of mouse endometrium with green fluorescent protein gene (GFP). Methods the endometrial cells of mice with GFP gene were isolated and collected. Sp cells were separated by flow cytometry. 2. The collected GFP endometrial cells and tissues were transplanted to the uterine serous and pelvic injury sites of mice. The experimental groups were divided into 2 groups: 1) transplanted endometrial cells group (2) transplanted endometrial cells and bone marrow mesenchymal cells co-cultured group 3) after pelvic peritoneal injury. After 3 weeks of pelvic peritoneal injury, the grafted endometrium microtissue group was kept for 4-6 weeks. The lesions were observed under fluorescence microscope and stained with HE and immunohistochemistry. Results the freshly digested single cells were stained with Hoechst, and SP cells were isolated by flow cytometry. The percentage of SP cells was 1.25-2.29. 2.Transplantation of fresh endometrial microtissue fragments containing GFP gene into normal ICR mice could obtain ectopic growth, and the predamaged mice had higher graft growth rate than the fresh injured mice. VS 37.5% were positive for GFP markers of transplanted growth. Conclusion: there are SP cells in mouse endometrial cells, microtissue transplantation is easier than cell transplantation to form transplantation focus, preinjury can improve the success rate of endometriosis transplantation.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R711.71;R-332

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相關(guān)期刊論文 前1條

1 胡菲菲;廖聯(lián)明;劉嘉茵;;產(chǎn)后小鼠子宮內(nèi)膜邊緣群干/祖細(xì)胞的分離純化[J];生殖醫(yī)學(xué)雜志;2008年04期

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