本文關鍵詞： 人類牙胚 SHH信號通路 轉錄因子 生長因子　出處：《福建師范大學》2010年碩士論文　論文類型：學位論文

【摘要】：SHH (Sonic Hedgehog)信號通路在小鼠早期牙胚的發(fā)育過程中起著關鍵的作用,對于其在小鼠磨牙中的分子生物學機制人們已經做了大量的研究。為了進一步探索SHH信號通路在人類牙胚發(fā)生發(fā)育過程中所起的調控作用以及可能的分子機制,本實驗率先檢測了SHH信號通路中的關鍵基因在人類牙胚發(fā)育早期的乳門牙和乳磨牙中的表達模式,這些基因包括了：SHH, PTCl, SMO, GLI1,GLI2以及GLI3,通過比對其在小鼠牙胚相應發(fā)育時期的表達模式來進一步揭示SHH信號通路在不同物種中的潛在表達調控機制。研究表明在蕾狀期以及帽狀期這些基因在人類與小鼠中的表達模式十分相近,但個別基因的表達模式存在差異,比如SHH信號在人類牙胚相應時期的表達區(qū)域就不局限在釉結節(jié)中,而是在整個內釉上皮中都可以檢測到其表達,而下游信號分子的表達區(qū)域范圍比小鼠中略大。在人類牙齒發(fā)育的鐘狀期SHH、PTCl的表達模式與小鼠中完全一致,但此時無論在人類的乳門牙還是乳磨牙中均檢測不到其它基因的表達。通過對本實驗結果的分析我們認為SHH信號通路在人類與小鼠牙胚發(fā)育的早期表達模式總體來說十分相近,很可能SHH信號通路在人類中以相同的調控模式調節(jié)著上皮和間充質細胞的增殖,誘導早期牙齒發(fā)育,而在人類牙齒的鐘狀期以后,SHH信號通路的調控作用很可能別別的家族的信號分子所取代,或者與其它分子通路之間形成新的信號轉導網(wǎng)絡。
[Abstract]:SHH Sonic Hedgehog signaling pathway plays a key role in the early development of mouse tooth germ. In order to further explore the regulatory role of SHH signaling pathway in the development of human tooth germs and possible molecular machineries, a great deal of research has been done on its molecular biological mechanism in mouse molars. System. This study was the first to detect the expression patterns of key genes in the SHH signaling pathway in human dental germs in the early stages of primary incisor and primary molars. These genes include: SHH, PTCl, SMO. GLI1, GLI2 and GLI3. By comparing their expression patterns in the corresponding developmental stages of mouse tooth germs, the potential expression regulation mechanism of SHH signaling pathway in different species was further revealed. The results showed that these genes were expressed in humans in bud and cap stages. The expression pattern was very similar to that in mice. However, the expression patterns of individual genes are different. For example, the expression region of SHH signal in the corresponding period of human tooth germ is not confined to the enamel nodule, but can be detected in the whole inner enamel epithelium. The expression range of downstream signal molecules was slightly larger than that of mice, and the expression pattern of SHHPCl in the bell stage of human tooth development was completely consistent with that in mice. However, no other genes were detected in human primary incisor or primary molars at this time. Based on the analysis of the results of this study, we believe that the expression pattern of SHH signaling pathway is early in the development of human and mouse tooth germs. It's very similar in general. It is very likely that the SHH signaling pathway regulates the proliferation of epithelial and mesenchymal cells in the same manner in humans and induces early tooth development after the bell phase of human teeth. The regulation of SHH signaling pathway is likely to be replaced by other family signaling molecules or to form a new signal transduction network with other molecular pathways.
【學位授予單位】：福建師范大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R321

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相關期刊論文 前1條

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本文編號：1477667