本文關(guān)鍵詞： 膝關(guān)節(jié)僵直 成纖維細(xì)胞樣滑膜細(xì)胞 核心蛋白多糖 I型膠原蛋白　出處：《中國矯形外科雜志》2015年13期 　論文類型：期刊論文

【摘要】：[目的]探討重組核心蛋白多糖(decorin)對培養(yǎng)的人僵直膝關(guān)節(jié)滑膜成纖維樣滑膜細(xì)胞(fibroblast-like synoviocytes,FLS)的增殖和Ⅰ型膠原蛋白及mRNA合成的影響,為Decorin治療人膝關(guān)節(jié)僵直提供理論依據(jù)。[方法]分離培養(yǎng)人僵直膝關(guān)節(jié)滑膜FLS,分別加入不同質(zhì)量濃度(10μg/ml,0.1μg/ml)的Decorin;培養(yǎng)24、48、72h后用二甲基噻唑二苯基四唑溴鹽(MTT)法測定FLS的增殖速度;用流式細(xì)胞儀檢測FLS的周期和凋亡;用RTPCR法檢測FLS合成Ⅰ型前膠原蛋白(PcⅠ)mRNA的含量,westem Blot檢測Ⅰ型膠原蛋白的合成量,并與對照組比較。[結(jié)果]10μg/ml的Decorin可有效抑制滑膜FLS的增殖(P0.05),明顯增加FLS處于G0/G1期的百分比(P0.05),并通過下調(diào)PcⅠmRNA的表達(dá),在轉(zhuǎn)錄水平上抑制PcⅠ的合成;實驗組和對照組均未檢測到終末期凋亡細(xì)胞。[結(jié)論]Decorin可抑制人僵直膝關(guān)節(jié)滑膜FLS的增殖及PcⅠmRNA的表達(dá)和Ⅰ型膠原蛋白合成,在防治膝關(guān)節(jié)僵直中起一定的作用。
[Abstract]:[Objective] to investigate the effect of recombinant core proteoglycan decorin (RCP) on cultured human rigid synovial fibroid synovial cells. Fibroblast-like synoviocytes. The effects of FLS on the synthesis of collagen type I and mRNA may provide theoretical basis for the treatment of knee joint stiffness with Decorin. [Methods] Human knee joint synovium FLS was isolated and cultured, and 10 渭 g / ml of 10 渭 g / ml (0.1 渭 g / ml) of Decorin was added respectively. The proliferation rate of FLS was determined by dimethyl thiazole diphenyl tetrazolium bromide salt method after 72 hours culture. The cycle and apoptosis of FLS were detected by flow cytometry. The content of procollagen 鈪，

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