本文關(guān)鍵詞： 介入方法 病態(tài)竇房結(jié)綜合征 大鼠模型 手術(shù)方法 病理　出處：《黑龍江中醫(yī)藥大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：(1)探索用介入法建立大鼠病態(tài)竇房結(jié)綜合征(sick sinus syndrome, SSS,簡稱病竇)模型的手術(shù)方法；(2)比較甲醛、氫氧化鈉、冰醋酸、無水乙醇、鴉膽子油這五種藥物哪種為介入法建立大鼠病竇模型的最佳試劑；(3)通過實驗中各種指標(biāo)的對比研究證明用介入法建立大鼠病竇模型比開胸法更優(yōu)越。 方法：先將一批健康大鼠篩查心電,選出心率在350-450次/分之間大鼠60只,然后隨機分為六組,分別是甲醛開胸濕敷組、甲醛介入組、氫氧化鈉介入組、冰醋酸介入組、無水乙醇介入組、鴉膽子油介入組,每組10只。甲醛開胸濕敷組：烏拉坦常規(guī)麻醉,連接呼吸機,開胸暴露竇房結(jié),用40%甲醛棉球濕敷竇房結(jié)區(qū)；甲醛介入組：無水乙醚麻醉,先將大鼠胸壁胸骨右緣第二肋間剪一小口,將自制標(biāo)測電極導(dǎo)管沿此小口插入胸腔,按照心電圖波形來確定給藥部位。40%甲醛溶液用微量注射器經(jīng)自制標(biāo)測電極導(dǎo)管局部滴注造模。其余介入組實驗方法同甲醛介入組。測量HR、進(jìn)行病理觀察、記錄術(shù)后存活率及術(shù)程并進(jìn)行對比研究。 結(jié)果：(1)與開胸法比較介入法術(shù)程短、操作更簡單且死亡率低；(2)各組大鼠心率均有不同程度下降,通過心率變化的統(tǒng)計分析,得出10%氫氧化鈉為最適合的造模藥物,效果穩(wěn)定；(3)從病理組織學(xué)分析來看,乙醇、冰醋酸、鴉膽子油雖然對竇房結(jié)有一定破壞,但損傷較輕；甲醛和氫氧化鈉對竇房結(jié)造成的損傷更接近病竇的臨床病理改變。 結(jié)論：1.用介入方法建立大鼠病竇模型方法簡便,定位準(zhǔn)確,副損傷小,成功率高。2.介入方法建立大鼠病竇模型的最佳試劑是10%的氫氧化鈉溶液,其建立的模型穩(wěn)定性好,可以用于較長時間的藥效學(xué)研究。
[Abstract]:Objective to explore the surgical method of establishing sick sinus syndrome (SSS) model of sick sinus syndrome (SSS) in rats by interventional method. (2) compare formaldehyde, sodium hydroxide, acetic acid, anhydrous ethanol and Brucea javanica oil, which is the best reagent to establish the rat model of sick sinus by interventional method; 3) the comparative study of various indexes in the experiment proved that the interventional method was superior to the open chest method in the establishment of the model of the diseased sinus in rats. Methods: a batch of healthy rats were screened and 60 rats with heart rate between 350 and 450 beats / min were selected. Then they were randomly divided into six groups: the open chest wet compress group and the formaldehyde intervention group. Sodium hydroxide intervention group, acetic acid intervention group, anhydrous ethanol intervention group, Brucea javanica oil intervention group, each group, 10 rats. Wet application of 40% formaldehyde cotton ball to sinus node area; Formaldehyde intervention group: anaesthetized with anhydrous ether, we cut the second intercostal part of the sternum of the rat chest wall first, and inserted the self-made measuring electrode catheter into the thoracic cavity along this small opening. According to the electrocardiogram waveform, the injection site of .40% formaldehyde solution was determined by micro-syringe. The experimental methods of other intervention groups were the same as those of formaldehyde intervention group and HR were measured. Pathological observation, postoperative survival rate and procedure were recorded and compared. Results compared with the open chest method, the intervention spell duration was shorter, the operation was simpler and the mortality rate was lower. (2) the heart rate of rats in each group decreased in different degrees. Through statistical analysis of heart rate changes, it was concluded that 10% sodium hydroxide was the most suitable drug for modeling, and the effect was stable. 3) from histopathological analysis, ethanol, glacial acetic acid and Brucea javanica oil damaged sinoatrial node slightly; The damage caused by formaldehyde and sodium hydroxide to the sinoatrial node is closer to the clinicopathological changes of the diseased sinus. Conclusion: 1.The method of establishing rat sick sinus model by interventional method is simple, accurate, less collateral damage and high success rate. The best reagent of interventional method is 10% sodium hydroxide solution. The established model has good stability and can be used in pharmacodynamics study for a long time.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R654.2;R-332

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