本文關鍵詞： 真菌性角膜炎 特異性免疫應答 免疫球蛋白 Th1型　出處：《青島大學》2009年碩士論文　論文類型：學位論文

【摘要】： 目的普遍認為非特異性免疫應答在真菌性角膜炎(FungalKeratitis,FK)發(fā)病和轉歸中起著至關重要的作用。本課題將探討實驗性的FK能否誘導特異性免疫應答。方法通過角膜基質注射白色念珠菌孢子建立小鼠的FK模型,鼻吸入白色念珠菌孢子誘導真菌性肺炎,皮下注射熱滅活的白色念珠菌孢子進行主動免疫。在初次或再次感染真菌性角膜炎后的不同時間點收集血漿,用ELISA測定IgA、IgG、IL4和IFNγ。用組織免疫熒光法檢測受染角膜組織沉積的免疫球蛋白和浸潤的淋巴細胞。結果免疫活性小鼠基質注射白色念珠菌孢子后發(fā)生典型的FK并在三周內痊愈。當再次用白色念珠菌孢子感染已痊愈的角膜時,相比于初次感染炎癥更輕并愈合更快。感染真菌性肺炎痊愈的小鼠和主動免疫的小鼠對FK的抵抗力更強。相比較于首次誘導FK的小鼠,以前接觸過白色念珠菌的小鼠再次誘導FK后血漿中產(chǎn)生更多的IgG和IgA含量,角膜中有更多的免疫球蛋白沉積和淋巴細胞浸潤。分析細胞因子表明FK誘導的免疫反應偏向于Th1型。結論FK誘導Th1型特異性免疫應答和免疫記憶,先前接觸過真菌制品提高宿主對其后角膜感染相同真菌的抵抗力。在高危人群中主動免疫可能是預防真菌性角膜炎的一種有效策略。
[Abstract]:The purpose is generally believed that the nonspecific immune response in fungal keratitis (FungalKeratitis, FK) incidence and outcome plays a crucial role. This paper will discuss the experimental FK can induce specific immune responses in mice. Methods FK model built by intrastromal injection of Candida albicans spores, nasal inhalation of Candida albicans induced by fungal spores the spores of Candida albicans pneumonia, subcutaneous injection of heat inactivated by active immunization. Plasma was collected at different time points of primary and secondary infection of fungal keratitis after determination of IgA, ELISA, IgG, IL4 and IFN with gamma. Immunofluorescence detection of immunoglobulin staining of corneal tissue deposition and infiltration of lymphocytes. The immune activity of mice after injection of matrix of Candida albicans spores typical FK and recovered in three weeks. When again with Candida albicans spores infection has been cured film angle When compared to the initial infection inflammation lighter and faster. The healing of fungal pneumonia in mice cured and active immunization of mice infected with resistance to FK stronger. For the first time compared to FK mice induced by IgG and IgA, the more content of plasma before contact with Candida albicans in mice induced by FK again, in the cornea immunoglobulin deposition and lymphocyte infiltration. More analysis of cytokines demonstrated that the immune response induced by FK in favor of Th1. Conclusion FK induced Th1 specific immune response and immune memory, previously exposed to fungal products improve host on subsequent corneal infection the same fungal resistance. In a high-risk population of active immunization may be a effective strategies for prevention of fungal keratitis.

【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R392.11;R772.2

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