本文關(guān)鍵詞： 真菌性角膜炎 特異性免疫應(yīng)答 免疫球蛋白 Th1型　出處：《青島大學(xué)》2009年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的普遍認(rèn)為非特異性免疫應(yīng)答在真菌性角膜炎(FungalKeratitis,FK)發(fā)病和轉(zhuǎn)歸中起著至關(guān)重要的作用。本課題將探討實(shí)驗(yàn)性的FK能否誘導(dǎo)特異性免疫應(yīng)答。方法通過(guò)角膜基質(zhì)注射白色念珠菌孢子建立小鼠的FK模型,鼻吸入白色念珠菌孢子誘導(dǎo)真菌性肺炎,皮下注射熱滅活的白色念珠菌孢子進(jìn)行主動(dòng)免疫。在初次或再次感染真菌性角膜炎后的不同時(shí)間點(diǎn)收集血漿,用ELISA測(cè)定IgA、IgG、IL4和IFNγ。用組織免疫熒光法檢測(cè)受染角膜組織沉積的免疫球蛋白和浸潤(rùn)的淋巴細(xì)胞。結(jié)果免疫活性小鼠基質(zhì)注射白色念珠菌孢子后發(fā)生典型的FK并在三周內(nèi)痊愈。當(dāng)再次用白色念珠菌孢子感染已痊愈的角膜時(shí),相比于初次感染炎癥更輕并愈合更快。感染真菌性肺炎痊愈的小鼠和主動(dòng)免疫的小鼠對(duì)FK的抵抗力更強(qiáng)。相比較于首次誘導(dǎo)FK的小鼠,以前接觸過(guò)白色念珠菌的小鼠再次誘導(dǎo)FK后血漿中產(chǎn)生更多的IgG和IgA含量,角膜中有更多的免疫球蛋白沉積和淋巴細(xì)胞浸潤(rùn)。分析細(xì)胞因子表明FK誘導(dǎo)的免疫反應(yīng)偏向于Th1型。結(jié)論FK誘導(dǎo)Th1型特異性免疫應(yīng)答和免疫記憶,先前接觸過(guò)真菌制品提高宿主對(duì)其后角膜感染相同真菌的抵抗力。在高危人群中主動(dòng)免疫可能是預(yù)防真菌性角膜炎的一種有效策略。
[Abstract]:The purpose is generally believed that the nonspecific immune response in fungal keratitis (FungalKeratitis, FK) incidence and outcome plays a crucial role. This paper will discuss the experimental FK can induce specific immune responses in mice. Methods FK model built by intrastromal injection of Candida albicans spores, nasal inhalation of Candida albicans induced by fungal spores the spores of Candida albicans pneumonia, subcutaneous injection of heat inactivated by active immunization. Plasma was collected at different time points of primary and secondary infection of fungal keratitis after determination of IgA, ELISA, IgG, IL4 and IFN with gamma. Immunofluorescence detection of immunoglobulin staining of corneal tissue deposition and infiltration of lymphocytes. The immune activity of mice after injection of matrix of Candida albicans spores typical FK and recovered in three weeks. When again with Candida albicans spores infection has been cured film angle When compared to the initial infection inflammation lighter and faster. The healing of fungal pneumonia in mice cured and active immunization of mice infected with resistance to FK stronger. For the first time compared to FK mice induced by IgG and IgA, the more content of plasma before contact with Candida albicans in mice induced by FK again, in the cornea immunoglobulin deposition and lymphocyte infiltration. More analysis of cytokines demonstrated that the immune response induced by FK in favor of Th1. Conclusion FK induced Th1 specific immune response and immune memory, previously exposed to fungal products improve host on subsequent corneal infection the same fungal resistance. In a high-risk population of active immunization may be a effective strategies for prevention of fungal keratitis.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類(lèi)號(hào)】：R392.11;R772.2

【同被引文獻(xiàn)】

相關(guān)期刊論文 前6條

1 張波,謝立信;真菌性角膜炎的動(dòng)物模型[J];國(guó)外醫(yī)學(xué)(眼科學(xué)分冊(cè));2005年03期

2 榮令;周新;;C型凝集素受體在抗真菌感染中作用的研究進(jìn)展[J];中國(guó)感染與化療雜志;2008年06期

3 蘇晶;李明飛;劉新泉;張殷建;;核苷酸結(jié)合寡聚化結(jié)構(gòu)域2在大鼠煙曲霉菌性角膜炎角膜局部的表達(dá)[J];上海醫(yī)學(xué);2008年12期

4 蘇晶;崔紅平;;大鼠煙曲霉菌性角膜炎模型的建立[J];同濟(jì)大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2006年03期

5 崔紅平;蘇晶;高新蕊;;大鼠煙曲霉菌性角膜炎角膜局部的免疫學(xué)研究[J];眼外傷職業(yè)眼病雜志(附眼科手術(shù));2007年08期

6 王麗婭,張?jiān)虑?王印其,王剛生,盧嘉彪,鄧潔華;中國(guó)三地區(qū)真菌性角膜病致病菌種的調(diào)查[J];中華眼科雜志;2000年02期

，

本文編號(hào)：1466707