本文關(guān)鍵詞： X-射線 放射性白內(nèi)障 αB-晶體蛋白　出處：《蘇州大學(xué)》2009年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的:利用動(dòng)物實(shí)驗(yàn),通過X射線照射S-D大鼠雙眼建立放射性白內(nèi)障動(dòng)物模型,觀察X射線劑量與白內(nèi)障嚴(yán)重程度之間的關(guān)系;檢測(cè)受不同劑量X射線照射的大鼠晶狀體中可溶性αB-晶體蛋白的表達(dá)水平,探討αB-晶體蛋白與放射性白內(nèi)障形成之間的關(guān)系。 方法:照射前選取8只大鼠作為正常對(duì)照組,實(shí)驗(yàn)照射組大鼠24只,平均分成3組每組8只,一次性分別照射X射線(雙眼同時(shí)照射)5、15、25 Gy.實(shí)驗(yàn)對(duì)照組24只,分成3組每組8只,分別作為5、15、25 Gy的對(duì)照組行假照射(照射光線中不含X射線,其它條件分別與對(duì)應(yīng)各照射組相同)。每只大鼠隨機(jī)取一只眼睛(本實(shí)驗(yàn)取右眼)為研究對(duì)象。所有大鼠分別于照射后1、3、5、7、15、30、45、60、90 d,腹腔注射3.6%水合氯醛麻醉后,用雙星明滴眼液充分散瞳,裂隙燈顯微鏡觀察并記錄晶狀體混濁情況;于照射后3個(gè)月將大鼠處死,取晶狀體作勻漿,取其上清液(可溶性晶體總蛋白)采用免疫印跡法(Western blot)檢測(cè)可溶性αB-晶體蛋白的含量。 結(jié)果:觀察3個(gè)月,正常對(duì)照組、各實(shí)驗(yàn)對(duì)照組和5 Gy照射組大鼠的晶狀體均未出現(xiàn)混濁。15 Gy照射組于照射后45 d晶狀體后囊和后皮質(zhì)開始出現(xiàn)細(xì)小、散在白點(diǎn)狀混濁,繼而融合成不均勻較密集點(diǎn)狀、片狀混濁,進(jìn)一步發(fā)展后皮質(zhì)出現(xiàn)較均勻致密混濁,前皮質(zhì)、前囊和核部輕度混濁。到3個(gè)月時(shí)62.5%的晶狀體達(dá)Ⅱ期混濁,37.5%達(dá)Ⅲ期混濁。25 Gy照射組亦出現(xiàn)類似變化,但是晶體發(fā)生混濁的時(shí)間更早、速度更快、混濁更明顯。于照射后30 d后囊即開始出現(xiàn)點(diǎn)狀混濁,此后病變迅速發(fā)展,到45 d時(shí)即有37.5%的晶體全部混濁。到3個(gè)月的時(shí)候除1例為后囊下明顯混濁外,其余7例晶狀體均完全混濁;15、25 Gy照射組可溶性晶體總蛋白濃度低于其它各組(P0.05);實(shí)驗(yàn)對(duì)照組之間可溶性αB-晶體蛋白含量的差異無統(tǒng)計(jì)學(xué)意義(P0.05),各實(shí)驗(yàn)照射組可溶性αB-晶體蛋白含量分別較其實(shí)驗(yàn)對(duì)照組降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05) ,3個(gè)實(shí)驗(yàn)照射組可溶性αB-晶體蛋白含量隨照射劑量的增加而逐漸降低,其差異有顯著統(tǒng)計(jì)學(xué)意義,(P0.05). 結(jié)論:大劑量X射線在短期內(nèi)即可引起晶狀體混濁;放射性白內(nèi)障的形成速度和程度與射線劑量成正相關(guān),在放射性白內(nèi)障中晶狀體內(nèi)可溶性αB-晶體蛋白表達(dá)減少,在放射性白內(nèi)障形成中起重要作用。
[Abstract]:Objective: to establish an animal model of radioactive cataract in the eyes of S-D rats by X-ray irradiation, and to observe the relationship between the dose of X-ray and the severity of cataract. The expression of soluble 偽 B crystal protein in rat lens irradiated with different doses of X ray was detected and the relationship between 偽 B crystal protein and radiation cataract formation was investigated. Methods: eight rats were selected as the normal control group before irradiation. 24 rats in the experimental group were divided into 3 groups with 8 rats in each group. 25 Gy.The experimental control group (n = 24) was divided into 3 groups (n = 8 in each group). The other conditions were the same as the corresponding irradiation groups. One eye was randomly selected from each rat (right eye in this experiment). 3.6% chloral hydrate was injected intraperitoneally for 90 days and the lens opacity was observed and recorded by slit lamp microscope. The rats were killed 3 months after irradiation and the lens was taken as homogenate. The supernatant (soluble crystal total protein) was used to detect the content of soluble 偽 B crystal protein by Western blot. Results: the control group was observed for 3 months. The lens of the experimental control group and the 5 Gy irradiation group did not appear opacity. The lens posterior capsule and posterior cortex of the 15 Gy irradiation group began to appear fine and scattered in the white dot shape after 45 days of irradiation. Then fusion into heterogeneous more dense dot shape, flake turbidity, after further development of the cortex appeared more homogeneous dense turbidity, precortex. The anterior capsule and nucleus were slightly opacified. By 3 months, 37.5% of the 62.5% lenses reached stage 鈪，

本文編號(hào)：1463721