本文關(guān)鍵詞：轉(zhuǎn)基因番茄可食防齲疫苗免疫大鼠的實(shí)驗(yàn)研究　出處：《重慶醫(yī)學(xué)》2015年23期 　論文類型：期刊論文

  更多相關(guān)文章： 番茄 植物 基因修飾 免疫療法 齲齒

【摘要】：目的用表達(dá)變異鏈球菌表面蛋白PAcP和霍亂毒素B亞單位融合蛋白的轉(zhuǎn)基因番茄免疫SD大鼠,檢測(cè)其免疫原性,探索研制安全、有效的可食用防齲疫苗的可能性。方法選擇雌性SD大鼠18只,建立齲齒模型,隨機(jī)分成3組(n=6),分別為轉(zhuǎn)基因番茄組(實(shí)驗(yàn)組)、變異鏈球菌滅活全菌免疫組(陽性對(duì)照組)、非轉(zhuǎn)基因番茄組(陰性對(duì)照組),免疫方式為灌胃免疫,每周免疫1次,連續(xù)免疫4周。分別于首次免疫前1d和每次免疫1周后采集血液、唾液樣品,用酶聯(lián)免疫吸附實(shí)驗(yàn)法檢測(cè)血清中免疫球蛋白G(IgG)、唾液中分泌型免疫球蛋白A(SIgA)抗體水平;鼠齡70d時(shí)處死動(dòng)物,并取上下頜骨進(jìn)行齲齒計(jì)分。結(jié)果免疫后,實(shí)驗(yàn)組和陽性對(duì)照組大鼠血清中IgG、唾液中SIgA抗體水平與陰性對(duì)照組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05);實(shí)驗(yàn)組和陰性對(duì)照組在Dx級(jí)外的各級(jí)差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論轉(zhuǎn)基因番茄防齲疫苗具有免疫原性,能夠誘導(dǎo)實(shí)驗(yàn)動(dòng)物產(chǎn)生有效的免疫應(yīng)答,降低齲齒的發(fā)生。
[Abstract]:Objective to immunize SD rats with transgenic tomato expressing Streptococcus mutans surface protein PAcP and cholera toxin B subunit fusion protein. Methods 18 female SD rats were selected to establish dental caries model and were randomly divided into 3 groups: transgenic tomato group (experimental group). Streptococcus mutans inactivated whole bacteria immunization group (positive control group, non-transgenic tomato group (negative control group), the way of immunization was intragastric immunization, once a week. The blood and saliva samples were collected 1 day before the first immunization and 1 week after each immunization. The serum immunoglobulin G G G g was detected by enzyme linked immunosorbent assay (Elisa). The level of secretory immunoglobulin A Siga antibody in saliva; The animals were killed at 70 days of age and the upper mandible was taken to score the dental caries. Results after immunization, IgG in serum of the experimental group and the positive control group was obtained. The level of SIgA antibody in saliva was significantly higher than that in negative control group (P 0.05). The difference between the experimental group and the negative control group was statistically significant (P 0.05). Conclusion transgenic tomato anti-caries vaccine has immunogenicity and can induce an effective immune response in experimental animals. Reduce the incidence of dental caries.
【作者單位】： 遵義醫(yī)學(xué)院口腔學(xué)院;貴州省高等學(xué)�？谇患膊⊙芯刻厣攸c(diǎn)實(shí)驗(yàn)室;貴陽市口腔醫(yī)院口腔內(nèi)科;遼河油田總醫(yī)院口腔科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(30160086) 貴州省第6批科技創(chuàng)新團(tuán)隊(duì)資助項(xiàng)目[黔科合人才團(tuán)隊(duì)(2013)4026號(hào)] 貴州省高等學(xué)校重點(diǎn)學(xué)科建設(shè)項(xiàng)目(SZXK-201207-04)
【分類號(hào)】：R392
【正文快照】： 齲病是人類最常見的細(xì)菌感染性疾病之一。就病因而言,細(xì)菌是齲病發(fā)病的主要因素,變異鏈球菌(S.mutans)表面蛋白PAc是被認(rèn)為重要的毒力因子。PAc的P區(qū)是脯氨酸富集區(qū),P區(qū)多肽片段中含有B細(xì)胞表位,具有免疫原性和免疫反應(yīng)性[1]。防齲疫苗經(jīng)歷了包括細(xì)菌活載體疫苗[2]、亞單位疫

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