本文關(guān)鍵詞：人臍帶血內(nèi)皮祖細(xì)胞的培養(yǎng)與鑒定　出處：《吉林大學(xué)》2008年碩士論文　論文類型：學(xué)位論文

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【摘要】： 血管內(nèi)皮祖細(xì)胞(endothelial progenitor cells,EPCs)是指具有特異性歸巢于血管新生組織并能分化增殖為成熟內(nèi)皮細(xì)胞的一群干/祖細(xì)胞,是中胚層卵黃囊血島原血干細(xì)胞(hemangioblast)向內(nèi)皮細(xì)胞分化過程中的一個(gè)過渡階段,該細(xì)胞在出生后的新生血管發(fā)生/生成中起重要作用。本實(shí)驗(yàn)從既簡化實(shí)驗(yàn)步驟又可以獲得足量內(nèi)皮祖細(xì)胞的角度出發(fā),通過6%羥乙基淀粉(Hydroxyethyl Starch, HES)沉降法和Percoll非連續(xù)性密度梯度離心法聯(lián)合應(yīng)用,分離、培養(yǎng)臍靜脈血中單個(gè)核細(xì)胞,在VEGF、bFGF、BPE等因子的體外誘導(dǎo)下,單個(gè)核細(xì)胞分化成為內(nèi)皮祖細(xì)胞,利用免疫組織化學(xué)、免疫熒光、流式細(xì)胞儀等技術(shù),從形態(tài)學(xué)、表面標(biāo)志以及功能特性等多角度逐漸深入地研究單個(gè)核細(xì)胞成功的分化成為內(nèi)皮祖細(xì)胞,并且進(jìn)一步向內(nèi)皮細(xì)胞分化的過程。本實(shí)驗(yàn)為進(jìn)一步體內(nèi)移植內(nèi)皮祖細(xì)胞,研究其在視網(wǎng)膜新生血管中的作用機(jī)制及尋找有效的防治措施提供了細(xì)胞來源。
[Abstract]:Endothelial progenitor cells (endothelial progenitor cells, EPCs) is a specific homing to angiogenesis and tissue differentiation into mature endothelial cells of a group of stem / progenitor cells, mesoderm is the yolk sac blood Shimabara blood stem cells (Hemangioblast) into endothelial cells in a transitional stage in the process of neovascularization in the cells in the postnatal / play an important role in the generation of this experiment. From the experimental procedure is simplified and can get sufficient endothelial progenitor cells through the perspective of 6% hydroxyethyl starch (Hydroxyethyl, Starch, HES) Percoll non precipitation method and combined application of continuous density gradient centrifugation and cultured mononuclear cells, umbilical vein the blood in VEGF, bFGF, BPE and other factors induced in vitro, differentiation of mononuclear cells into endothelial progenitor cells by immunohistochemistry, immunofluorescence, flow cytometry, from morphology, Surface markers and functional properties such as multi angle gradually in-depth study on differentiation of mononuclear cells into endothelial progenitor cells successfully, and further differentiation into endothelial cells. This experiment for further in vivo transplantation of endothelial progenitor cells, cell sources for research and find out the effective control measures in retinal neovascularization mechanism.

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R329

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前2條

1 石慧;內(nèi)皮祖細(xì)胞眼內(nèi)移植的示蹤及對視網(wǎng)膜血管損傷修復(fù)的研究[D];吉林大學(xué);2011年

2 衛(wèi)肖艷;電針對腦缺血再灌注大鼠bFGF、CD34表達(dá)及EPCs功能的影響[D];北京中醫(yī)藥大學(xué);2013年

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本文編號：1410543