本文關(guān)鍵詞：腦區(qū)硫胺素缺乏對AD模型小鼠腦神經(jīng)元線粒體功能的影響　出處：《神經(jīng)解剖學(xué)雜志》2015年05期 　論文類型：期刊論文

  更多相關(guān)文章： 阿爾茨海默病 硫胺素缺乏 線粒體功能障礙 VDAC cytochrome C 小鼠

【摘要】：目的:檢測阿爾茨海默病(AD)模型小鼠腦區(qū)硫胺素缺乏后,電壓依賴性陰離子通道蛋白1(VDAC1)和細胞色素C(cytochrome C)蛋白表達的變化。方法:選用2~3月齡阿爾茨海默病模型小鼠(APP/PS1雙轉(zhuǎn)基因小鼠)和野生型(WT)小鼠,根據(jù)小鼠腦圖譜用立體定位注射法在小鼠右海馬齒狀回,右前皮質(zhì)腦區(qū)注射維生素B1拮抗劑,導(dǎo)致硫胺素缺乏(TD)。在TD處理后10 d進行動物行為學(xué)檢測,TD處理后30 d應(yīng)用免疫熒光、Western Blot及RT-PCR法檢測VDAC1和細胞色素C在小鼠注射腦區(qū)蛋白的表達和分布并分析線粒體總DNA(mt DNA)的變化。結(jié)果:TD處理后APP/PS1小鼠和WT小鼠的主,被動規(guī)避行為與對照組相比有顯著下降(P0.05),兩組小鼠注射腦區(qū)的VDAC1和細胞色素C呈現(xiàn)高表達(P0.05),腦組織mt DNA總量增加(P0.05)。結(jié)論:硫胺素缺乏可以導(dǎo)致AD模型小鼠和野生型小鼠腦內(nèi)線粒體功能發(fā)生改變。
[Abstract]:Objective: to detect thiamine deficiency in brain region of Alzheimer's disease (AD) mice. Changes in the expression of VDAC1 and cytochrome C in voltage-dependent anion channel protein 1 (VDAC1). Methods: two or three months old mice with Alzheimer's disease (AD) were selected. APP/PS1 double transgenic mice and wild-type WT-mice. According to the mouse brain map, vitamin B1 antagonist was injected into the right hippocampal dentate gyrus and right anterior cortical area of mice by stereotactic injection. The results showed that thiamine was deficient in TD. immunofluorescence was used at 10 days after TD treatment and 30 days after TD treatment. Expression and distribution of VDAC1 and cytochrome C protein in mouse brain were detected by Western Blot and RT-PCR method, and total mitochondrial DNA(mt DNA was analyzed. Results APP/PS1 mice and WT mice were treated with TD-treated APP/PS1 and WT mice. Compared with the control group, the passive circumvention behavior decreased significantly (P 0.05), and the VDAC1 and cytochrome C in the brain area of the two groups showed high expression (P 0.05). Conclusion: thiamine deficiency can result in changes of mitochondrial function in AD model mice and wild-type mice.
【作者單位】： 鄭州大學(xué)基礎(chǔ)醫(yī)學(xué)院人體解剖學(xué)教研室;
【基金】：國家自然科學(xué)基金(81401015)
【分類號】：R749.16;R-332
【正文快照】： 腦區(qū)硫胺素缺乏對AD模型小鼠腦神經(jīng)元線粒體功能的影響王靜,常成,張俊霞,曹靜靜,方志慧,高曉群*(鄭州大學(xué)基礎(chǔ)醫(yī)學(xué)院人體解剖學(xué)教研室,鄭州450001)阿爾茨海默病(Alzheimer's disease,AD)是最為常見的神經(jīng)退行性疾病,其主要病理特點是廣泛的神經(jīng)元與突觸丟失,神經(jīng)原纖維纏結(jié)(NF

【共引文獻】

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