本文關(guān)鍵詞：兔脊神經(jīng)后支切斷脊柱側(cè)彎動物模型建立　出處：《中國醫(yī)科大學》2009年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 脊柱側(cè)彎 椎旁肌 失神經(jīng)支配 兔

【摘要】： 目的 特發(fā)性脊柱側(cè)彎作為一種復雜的三維畸形,其病因?qū)W機制至今未明。椎旁肌作為軀干反射的最終效應器官,椎旁肌病理改變與脊柱側(cè)彎的關(guān)系一直是人們關(guān)心熱點。椎旁肌作為背部固有肌群,其作用為維持姿勢和控制脊柱的運動。脊神經(jīng)后支是由脊神經(jīng)前、后根在椎管內(nèi)匯合后,于椎間孔水平發(fā)出支配椎旁肌的混合神經(jīng),含有感覺和運動兩種神經(jīng)纖維。本實驗通過在椎間孔水平切斷單側(cè)不同節(jié)段、不同數(shù)量的脊神經(jīng)后支,造成單側(cè)椎旁肌失神經(jīng)支配,觀察是否形成凸向失神經(jīng)支配側(cè)脊柱側(cè)彎畸形。探討椎旁肌失神經(jīng)支配對脊柱側(cè)彎發(fā)生發(fā)展的影響。 材料與方法 1、實驗動物 日本大耳白兔70只,雌雄各半,體重500-700克,兔齡4-6周。隨機分為16組,每組5只。實驗組40只,對照組30只。 2、實驗方法 水合氯醛3-5ml/kg腹腔注射麻醉。麻醉后俯臥位常規(guī)備皮、消毒,選取后背正中切口,長約(3-6)cm。于上關(guān)節(jié)突旁2mm處切開豎脊肌筋膜(圖15-16),順著肌纖維走形垂直鈍性分離肌肉(不切除切斷肌肉),范圍約4mm。實驗組均行右側(cè)脊神經(jīng)后支切斷、切除1cm神經(jīng)組織,左側(cè)不做任何操作。實驗組*號組右側(cè)脊神經(jīng)后支切斷、切除1cm神經(jīng)組織,左側(cè)手術(shù)暴露神經(jīng)同右側(cè),但不切斷切除神經(jīng)。對照組均行右側(cè)脊神經(jīng)后支暴露術(shù),不切斷切除神經(jīng)。對照組*號組雙側(cè)均行脊神經(jīng)后支暴露術(shù),不切斷切除神經(jīng)。 3、結(jié)果判定 術(shù)后將切除物行HE染色觀察是否為神經(jīng)組織。術(shù)后1、2、3月攝片觀察脊柱彎曲變化。術(shù)后3月椎旁肌裸視下觀察和HE染色觀察神經(jīng)切斷側(cè)肌肉是否萎縮。 結(jié)果 組織學檢查證實切除為神經(jīng)組織,術(shù)后3月椎旁肌裸視下觀察及HE染色見神經(jīng)切斷側(cè)肌肉明顯萎縮,但各實驗組均未發(fā)現(xiàn)有凸向失神經(jīng)支配側(cè)的脊柱側(cè)彎畸形。部分兔形成凸向失神經(jīng)支配對側(cè)的脊柱側(cè)彎畸形。 結(jié)論 1、通過該方法造成兔單側(cè)椎旁肌失神經(jīng)支配,不能誘發(fā)凸向失神經(jīng)支配側(cè)脊柱側(cè)彎畸形。 2、IS患者椎旁肌失神經(jīng)支配為脊柱側(cè)彎的病因可能性較小。 3、單純的椎旁肌失神經(jīng)支配,雖可引起相應節(jié)段雙側(cè)肌力不一致,但在平衡調(diào)節(jié)功能正常的情況下,可通過神經(jīng)反射對未失神經(jīng)的肌肉進行代償性調(diào)節(jié),維持脊柱兩側(cè)的相對平衡,而不發(fā)生脊柱側(cè)彎畸形。因此推測人類特發(fā)性脊柱側(cè)彎的發(fā)生發(fā)展可能是多因素紊亂的共同結(jié)果。 4、部分兔形成凸向失神經(jīng)支配對側(cè)的脊柱側(cè)彎畸形,考慮為術(shù)后肌肉瘢痕攣縮所致。
[Abstract]:objective
Idiopathic scoliosis is a complex three-dimensional deformity, its etiology remains unknown. As the final effect of paraspinal muscle trunk reflexgan, relationship between the pathological changes of paravertebral muscle and scoliosis is the most concerned topic. As the paraspinal muscle back muscles with its inherent, in order to maintain posture and to control the movement of the spine. The posterior branch of spinal nerve by spinal nerve root, after confluence in the spinal canal, intervertebral foramen in a mixed level of innervation of the paraspinal muscles, containing two kinds of sensory and motor nerve fibers. The experiments of different segments in unilateral transection of the intervertebral foramen, different number of spinal nerve after, caused unilateral paraspinal muscle denervation, observe whether the formation of convex to the denervated side of scoliosis. To investigate the effects of denervation of paravertebral muscles on the occurrence and development of scoliosis.
Materials and methods
1, experimental animals
70 Japanese white rabbits, with half female and male, 500-700 grams of weight, and 4-6 weeks of old age, were randomly divided into 16 groups, 5 in each group, 40 in the experimental group and 30 in the control group.
2, experimental method
3-5ml/kg intraperitoneal injection of chloral hydrate anesthesia. Anesthesia prone position after routine disinfection, skin preparation, select back incision, ca. (3-6) cm. on juxtafacet 2mm incision of erector spinae fascia (Figure 15-16), the muscle fibers take vertical blunt separation of muscles (not cut muscle resection, cut off the branch) the range of about 4mm. experiments were performed on the right side of the spinal nerve after resection of 1cm nerve tissue, left without any operation. The experimental group * group right posterior branch of spinal nerve was cut off, 1cm nerve tissue, the left nerve with the right surgical exposure, but do not cut off the nerve. The control group underwent resection of the right posterior branch of spinal nerve was exposed don't cut off, denervate. The control group * group underwent bilateral posterior branch of spinal nerve was exposed, do not cut off the nerve resection.
3, result determination
After excision for HE staining to observe whether nerve tissue. Radiographic observation of spinal curvature changes of 1,2,3 months after the operation. Observe the nerve lateral muscle atrophy after March paraspinal muscles were observed under naked eye and HE staining.
Result
Histological examination showed that the resection is the nerve tissue, and HE observed after March paraspinal muscle staining under bare nerve lateral muscle atrophy, but the experimental group were not found to have a convex spine to the denervated side bending deformity. Rabbit form convex to the denervated spinal column side to side bending deformity.
conclusion
1, by this method, the unilateral paravertebral denervation of the rabbit could not induce the scoliosis on the innervation side of the denervation.
2, the pathogeny of the paravertebral denervation in IS patients is less likely to be scoliosis.
3, simple paraspinal muscle denervation, can cause the corresponding bilateral segmental muscle strength is not the same, but in the balance function of normal, on denervated muscles compensatory adjustment by reflex, to maintain the relative balance on both sides of the spine, without spinal scoliosis. Therefore that human idiopathic scoliosis is a common result of the occurrence and development of possible multi factor disorders.
4, some rabbits form the contralateral scoliosis deformity of the contralateral innervation of the denervation, which is considered to be the result of postoperative muscle scar contracture.

【學位授予單位】：中國醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R682;R-332

【參考文獻】

相關(guān)期刊論文 前1條

1 馬軍,于鳳章,潘少川,張學軍,孫琳,李承鑫;骺阻滯誘導脊柱側(cè)彎動物模型的實驗研究[J];中華小兒外科雜志;2002年02期

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本文編號：1401262