本文關(guān)鍵詞：睡眠剝奪對大鼠血漿去甲腎上腺素、多巴胺、五羥色胺及認知功能的影響　出處：《福建醫(yī)科大學》2010年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 睡眠剝奪 去甲腎上腺素 多巴胺 五羥色胺 認知功能

【摘要】： 目的: 1、研究睡眠剝奪對大鼠血漿中去甲腎上腺素(NE)、多巴胺(DA)、五羥色胺(5-HT)含量及認知功能的影響。 2、探討NE、DA、5-HT與睡眠—覺醒周期的內(nèi)在聯(lián)系。 3、探討睡眠剝奪影響認知功能的可能機制。 方法: 健康雄性8～12周齡的Sprague-Dawley(SD)大鼠27只,通過隨機數(shù)字表分為三組:(1)小平臺睡眠剝奪組;(2)大平臺環(huán)境對照組;(3)空白對照組。采用改良多平臺睡眠剝奪法建立睡眠剝奪模型,睡眠剝奪前后用Morris水迷宮視頻跟蹤分析系統(tǒng)檢測三組大鼠的認知功能水平。采用腹主動脈取血法收集血漿,沉淀蛋白質(zhì),利用高效液相電化學法檢測血漿中NE、DA、5-HT的含量。 結(jié)果: 1、血漿中NE、DA、5-HT的檢測結(jié)果:(1)NE:睡眠剝奪組的含量比環(huán)境對照組、空白對照組的含量高,差異有統(tǒng)計學意義(p值分別為0.033、0.034),環(huán)境對照組與空白對照組的含量無明顯差異。(2)DA:睡眠剝奪組的含量較環(huán)境對照組、空白對照組的含量低,差異有統(tǒng)計學意義(p值分別為0.034、0.035),環(huán)境對照組與空白對照組的含量無明顯差異。(3)5-HT:睡眠剝奪組的含量較環(huán)境對照組、空白對照組的含量高,差異有統(tǒng)計學意義(p值分別為0.036、0.035),環(huán)境對照組與空白對照組的含量無明顯差異。 2、Morris水迷宮檢測大鼠認知功能的結(jié)果:(1):睡眠剝奪前,睡眠剝奪組、環(huán)境對照組和空白對照組的潛伏期無統(tǒng)計學差異。(2):睡眠剝奪后,睡眠剝奪組、環(huán)境對照組和空白對照組三組的潛伏期均下降,差異有統(tǒng)計學意義(p值分別為0.047、0.045、0.043)。(3):睡眠剝奪后,睡眠剝奪組的潛伏期比環(huán)境對照組、空白對照組的潛伏期明顯較長,差異有統(tǒng)計學意義(p值分別為0.044、0.043),環(huán)境對照組和空白對照組的潛伏期無統(tǒng)計學差異。 結(jié)論: 1、睡眠剝奪影響體內(nèi)神經(jīng)遞質(zhì)的含量,使血漿中NE、5-HT水平明顯升高,DA水平下降。 2、睡眠剝奪使機體的認知功能下降。 3、體內(nèi)NE、DA、5-HT含量的改變可能與睡眠—覺醒周期存在一定的聯(lián)系。 4、睡眠剝奪對認知功能的影響可能與體內(nèi)NE、DA、5-HT含量的改變相關(guān)。
[Abstract]:Objective: 1. To study the effects of sleep deprivation on the contents of norepinephrine, dopamine, serotonin (5-HT) and cognitive function in plasma of rats. 2. To explore the inner relationship between NEDAA 5 HT and sleep-wakefulness cycle. 3. To explore the possible mechanism of sleep deprivation affecting cognitive function. Methods: Twenty-seven healthy male Sprague-Dawley (SD) rats aged 8 to 12 weeks were randomly divided into three groups: 1) small platform sleep deprivation group; 2) large platform environment control group; 3) blank control group. Sleep deprivation model was established by modified multi-platform sleep deprivation method. The cognitive function of the three groups was measured by Morris water maze video tracking analysis system before and after sleep deprivation. Plasma was collected and protein was precipitated by abdominal aorta blood sampling. A high performance liquid-phase electrochemical method was used to determine the content of neodadine 5-HT in plasma. Results: The results showed that the content of NED 5-HT in plasma was higher in the sleep deprivation group than in the environmental control group and the blank control group. The difference was statistically significant (P = 0.033, 0.034). There was no significant difference between the environmental control group and the blank control group. The content of sleep deprivation group was higher than that of the environment control group. The content of blank control group was low, the difference was statistically significant (0.034% 0.035). There was no significant difference between the environmental control group and the blank control group. The content of sleep deprivation group was higher than that of the environment control group, and the content of blank control group was higher. The difference was statistically significant (P = 0.036). There was no significant difference between the environmental control group and the blank control group. 2 the results of Morris water maze test of cognitive function in rats: 1: 1: before sleep deprivation, sleep deprivation group. There was no significant difference in latency between the environmental control group and the blank control group: after sleep deprivation, the latency of the sleep deprivation group, the environment control group and the blank control group were all decreased. The difference was statistically significant: the latency of sleep deprivation group was higher than that of environment control group after sleep deprivation. The incubation period of the blank control group was longer than that of the control group, and the difference was statistically significant (0.044 鹵0.043), while the latency of the environmental control group and the blank control group had no statistical difference. Conclusion: 1. Sleep deprivation affected the content of neurotransmitters in vivo and significantly increased the level of NE-5-HT in plasma and decreased DA level. 2. Sleep deprivation reduces the cognitive function of the body. 3. The change of NED 5-HT may be related to the sleep-wakefulness cycle. 4. The effect of sleep deprivation on cognitive function may be related to the change of NED 5 HT content.
【學位授予單位】：福建醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R363

【引證文獻】

相關(guān)碩士學位論文 前3條

1 王平;自我按摩訓練對老年人睡眠質(zhì)量及認知功能的效果研究[D];中南大學;2011年

2 蘇聰慧;加味酸h仁湯對模擬運動性失眠大鼠血漿單胺類神經(jīng)遞質(zhì)的研究[D];曲阜師范大學;2012年

3 邱蓓蕾;運動性失眠大鼠建模初探[D];曲阜師范大學;2012年

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本文編號：1398591