本文關(guān)鍵詞：呼吸道合胞病毒培養(yǎng)及其病毒滴度檢測方法的建立　出處：《中國生物制品學(xué)雜志》2015年09期 　論文類型：期刊論文

  更多相關(guān)文章： 呼吸道合胞病毒 培養(yǎng)條件 病毒滴度

【摘要】：目的確定呼吸道合胞病毒(respiratory syncytial virus,RSV)培養(yǎng)條件,篩選病毒保護劑配方,并建立檢測病毒滴度的微量細(xì)胞病變方法。方法分別將Hep2、Vero和293R細(xì)胞以0.01 MOI接種RSV,選擇病毒培養(yǎng)細(xì)胞基質(zhì);將RSV分別以0.005和0.02 MOI接種Hep2細(xì)胞,在4~9 d收獲病毒液,檢測病毒滴度,確定最佳MOI及病毒收獲時間;將6種配方病毒保護劑分別加至病毒液中,反復(fù)凍融7次,檢測病毒滴度,篩選最佳配方;將病毒分別在33和37℃條件下滴定,第7、9天判定結(jié)果,確定微量細(xì)胞病變方法的培養(yǎng)溫度和判定時間。結(jié)果確定病毒培養(yǎng)細(xì)胞基質(zhì)為Hep2細(xì)胞,以0.02 MOI RSV接種后,37℃培養(yǎng)7~9 d收獲病毒液;配方1(0.1%人血白蛋白)為最佳病毒保護劑配方;微量細(xì)胞病變法的實驗條件為37℃滴定,7 d判定結(jié)果。結(jié)論建立了穩(wěn)定可靠的呼吸道合胞病毒培養(yǎng)及病毒滴度檢測方法。
[Abstract]:Objective to determine the culture conditions of respiratory syncytial virus (RSV) and to screen the formula of virus protection agent. Methods Hep2Vero and 293R cells were inoculated with RSV0.01 MOI to select the cell matrix of virus culture. RSV was inoculated with 0. 005 and 0. 02 MOI respectively. The virus solution was harvested at 4 ~ 9 days. The virus titer was detected to determine the best MOI and harvest time. Six kinds of viral protectants were added to the virus solution for 7 times. The titer of virus was detected and the best formula was screened. The virus was titrated at 33 鈩，

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