無(wú)錫市副溶血性弧菌感染的分子流行病學(xué)特征分析
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本文關(guān)鍵詞:無(wú)錫市副溶血性弧菌感染的分子流行病學(xué)特征分析 出處:《中國(guó)病原生物學(xué)雜志》2015年07期 論文類型:期刊論文
更多相關(guān)文章: 副溶血性弧菌 腹瀉 脈沖場(chǎng)凝膠電泳
【摘要】:目的了解無(wú)錫市腹瀉人群中副溶血性弧菌感染的流行狀況及分子特征。方法對(duì)無(wú)錫市30家哨點(diǎn)醫(yī)院感染性腹瀉患者的糞便標(biāo)本分別進(jìn)行副溶血性弧菌的分離鑒定、血清學(xué)分型、毒力基因檢測(cè)以及脈沖場(chǎng)凝膠電泳(PFGE)圖譜分析。結(jié)果在1 238例標(biāo)本中共分離到31株副溶血性弧菌,陽(yáng)性率為2.50%;檢出時(shí)間主要集中在7~9月,以8月份的檢出率最高。使用PCR方法分別檢測(cè)31株副溶血性弧菌的跨膜轉(zhuǎn)錄激活蛋白基因(tox R)、耐熱直接溶血素基因(tdh)和耐熱相關(guān)溶血素基因(trh),毒力基因tox R+、tdh+、trh+攜帶率分別為100%、100%和3.23%。PFGE圖譜顯示,27株O3㑳K6血清型的副溶血性弧菌共有4種PFGE帶型,88.89%的副溶血性弧菌為同一種帶型。結(jié)論無(wú)錫市副溶血性弧菌的感染具有季節(jié)性和人群分布的差異性,主要由同一種毒力強(qiáng)的O3㑳K6型菌株引起,具有產(chǎn)生暴發(fā)流行的可能,需進(jìn)一步加強(qiáng)監(jiān)測(cè)管理。
[Abstract]:Objective to investigate the epidemic status and molecular characteristics of vibrio parahaemolyticus infection in diarrhea population in Wuxi. Methods the fecal samples of 30 patients with infectious diarrhea in sentinel hospitals in Wuxi city were isolated from Vibrio parahaemolyticus. Yes. Results 31 strains of Vibrio parahaemolyticus were isolated from 1 238 samples, the positive rate was 2.50. The detection time was mainly from 7 to September, with the highest detection rate in August. 31 strains of Vibrio parahaemolyticus transmembrane transcriptional activator gene (TORX R) were detected by PCR. The carrying rates of heat tolerance direct hemolysin gene (THD) and heat-resistant hemolysin gene (TDH) and virulence gene tox R (TDH) were 100%, respectively. The 100% and 3.23. PFGE profiles showed 27 strains of O3? Vibrio parahaemolyticus serotype K6 has four PFGE bands. Conclusion the infection of Vibrio parahaemolyticus in Wuxi city has seasonal and population distribution differences, mainly caused by the same virulence of O _ 3? It is possible to cause outbreak of K 6 strain, so surveillance and management should be further strengthened.
【作者單位】: 無(wú)錫市疾病預(yù)防控制中心;
【分類號(hào)】:R378.3
【正文快照】: *副溶血性弧菌(Vibrio parahaemolyticus,VP)是一種廣泛存在于海產(chǎn)品中的食源性致病菌,人食用被該菌污染的產(chǎn)品后會(huì)出現(xiàn)腹瀉、腸痙攣、惡心、嘔吐等胃腸炎反應(yīng),嚴(yán)重者可發(fā)生休克,且能引起重復(fù)感染[1]。在日本、美國(guó)、臺(tái)灣等沿海國(guó)家和地區(qū),細(xì)菌性食源性疾病暴發(fā)事件的一半以上
【參考文獻(xiàn)】
相關(guān)期刊論文 前8條
1 王海波;王多春;闞飆;畢振強(qiáng);;副溶血弧菌TaqMan雙重實(shí)時(shí)-聚合酶鏈反應(yīng)檢測(cè)方法的建立[J];疾病監(jiān)測(cè);2009年04期
2 黎薇;方偉;柯昌文;張萬(wàn)里;曾漢日;馬聰;朱海明;;廣東省2003~2008年副溶血性弧菌血清學(xué)分型研究[J];中國(guó)病原生物學(xué)雜志;2009年07期
3 陸建榮;何連生;;食物中毒副溶血性弧菌的分離及分型研究[J];中國(guó)病原生物學(xué)雜志;2010年06期
4 郭川;林奕華;莊旭冬;孫作O,
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