【摘要】：目的結(jié)扎大鼠冠狀動脈前降支造成大鼠實驗性急性心肌梗死(AMI)模型,觀察聯(lián)合應(yīng)用阿司匹林和氯吡格雷對AMI后大鼠胃黏膜微血管的影響,探討雙聯(lián)抗致大鼠胃黏膜損傷可能的藥理作用機制。方法將SD大鼠腹腔麻醉(4%水合氯醛,9 m L·kg-1體質(zhì)量)后常規(guī)處理,結(jié)扎左冠狀動脈前降支血管造成大鼠實驗性AMI模型,確定成活的大鼠隨機分為(1)模型(AMI)對照組:灌胃蒸餾水2 mg·kg-1·d-1;(2)阿司匹林組:灌胃阿司匹林9 mg·kg-1·d-1;(3)氯吡格雷組:灌胃氯吡格雷6.75 mg·kg-1·d-1;(4)雙聯(lián)抗組:灌胃阿司匹林9mg·kg-1·d-1和氯吡格雷6.75 mg·kg-1·d-1混懸液。另取12只大鼠作為假手術(shù)組(只穿刺不結(jié)扎冠脈,其余處理方法與AMI模型組相同),每組12只,雌雄各半,連續(xù)灌胃28 d后,第29 d進行微血管形態(tài)的觀察,實驗結(jié)束后腹主動脈取血,放射免疫法測定胃泌素(GAS)、胃動素(MTL)和6-酮-前列腺素F1α(6-keto-PGF1α)和血栓素B2(TXB2)指標,大鼠胃黏膜分別按照Guth標準和Whittle方法評定損傷指數(shù)和胃黏膜損傷程度。結(jié)果經(jīng)Guth方法和Whittle標準的計分評定和假手術(shù)組比較,小劑量阿司匹林組大鼠胃黏膜損傷及程度明顯,阿司匹林聯(lián)合氯吡格雷后胃黏膜損傷程度加重,胃黏膜微血管擴張,血管壁變薄,毛細血管交叉數(shù)減少顯著(P0.05),大鼠血漿GAS水平顯著降低(P0.01),MTL水平顯著升高(P0.01)。阿司匹林組的GAS降低水平和MTL升高水平大于氯吡格雷組,和假手術(shù)組比較,阿司匹林組可以顯著降低TXB2水平。結(jié)論阿司匹林和氯吡格雷聯(lián)合應(yīng)用可加重AMI后大鼠胃黏膜的損傷,其機制與胃腸激素水平紊亂、干擾前列腺素合成導(dǎo)致胃黏膜微血管障礙密切相關(guān)。
[Abstract]:Objective to observe the effect of aspirin and clopidogrel on gastric mucosal microvessels in rats with experimental acute myocardial infarction (AMI) induced by ligating the anterior descending branch of coronary artery in rats. To explore the possible pharmacological mechanism of double anti-gastric mucosal injury in rats. Methods the experimental AMI model was established by ligating the anterior descending branch of the left coronary artery after intraabdominal anesthesia (4% chloral hydrate, 9 mL kg-1 body mass) in SD rats. The surviving rats were randomly divided into (1) model (AMI) control group: distilled water was given intragastrically 2 mg kg- 1 路d-1; (2) aspirin group: aspirin 9 mg kg- 1 路d-1, clopidogrel 6.75 mg kg- 1 路d-1; (4) double antibody group: aspirin 9mg kg- 1 路d-1 and clopidogrel 6.75 mg kg- 1 路d-1 suspension were given intragastrically. Another 12 rats were taken as false operation group (only puncture without ligating coronary artery, and the other treatments were the same as those in AMI model group). 12 rats in each group, half male and half female, were given intragastrically for 28 days, and the morphology of microvessels was observed on the 29th day. At the end of the experiment, blood was taken from abdominal aortic, and the indexes of (GAS), motilin (MTL), 6-keto-prostaglandin F1 偽 (6-keto-PGF1 偽) and thromboxane B2 (TXB2) were measured by radioimmunoassay. The injury index and degree of gastric mucosa injury were evaluated according to Guth standard and Whittle method, respectively. Results compared with the false operation group, the injury and degree of gastric mucosa in the low dose aspirin group were significantly higher than those in the false operation group by Guth method and Whittle standard score. The degree of gastric mucous membrane injury was aggravated and the microvessels of gastric mucosa were dilated after aspirin combined with clopidogrel. The blood vessel wall became thinner, the number of capillary crossing decreased significantly (P 0.05), and the plasma GAS level decreased significantly (P 0.01), MTL level). The decreased level of GAS and the increase of MTL in aspirin group were higher than those in clopidogrel group. Compared with the pseudo-operation group, aspirin group could significantly decrease the level of TXB2. Conclusion combination of aspirin and clopidogrel can aggravate the injury of gastric mucosa in rats after AMI, and its mechanism is closely related to the disorder of gastrointestinal hormone levels and the microvascular disorder of gastric mucosa caused by interference with prostaglandin synthesis.
【作者單位】： 中國中醫(yī)科學(xué)院西苑醫(yī)院中國中醫(yī)科學(xué)院心血管病研究所;山東省泰安市中醫(yī)醫(yī)院;
【基金】：科技部國際科技合作與交流項目(2014DFG32700,2010DFA31690) 國家自然科學(xué)基金(81273933)
【分類號】：R542.22

【相似文獻】

相關(guān)期刊論文 前10條

1 楊振芳,么淑珍;大鼠飲鹽行為的個體發(fā)生的研究[J];天津醫(yī)科大學(xué)學(xué)報;1999年02期

2 黃強;劉富強;牟建軍;;高鹽對SHR大鼠內(nèi)皮功能的影響[J];齊齊哈爾醫(yī)學(xué)院學(xué)報;2010年01期

3 張廷星;晉學(xué)慶;吳可貴;許昌聲;陳小明;;雌激素對SD大鼠血脂代謝的影響[J];高血壓雜志;2005年12期

4 惠海鵬,李小鷹,彭江;大鼠經(jīng)腹心包腔內(nèi)注射術(shù)的研究[J];醫(yī)學(xué)研究生學(xué)報;2005年10期

5 陳春林;鞏甜甜;湯依群;寇瑩瑩;于鵬;邵容;張長風(fēng);;SD大鼠房顫模型的建立[J];實驗動物科學(xué);2009年03期

6 劉佳佳;嚴進;鄭冬梅;唐云翔;潘霄;;恐懼應(yīng)激對大鼠心血管系統(tǒng)及一般行為的影響[J];中國健康心理學(xué)雜志;2011年09期

7 杜曉軍,高廣道,盧興,楊繼聲;大鼠左心室泵功能的電磁流量計測定[J];生理學(xué)報;1985年06期

8 羅浩;王新全;王甲良;陳碩;陳彩宇;曾春雨;;血管緊張素Ⅱ1型受體在肥胖相關(guān)性高血壓大鼠腎臟的表達及功能[J];中華高血壓雜志;2014年07期

9 林森;趙連東;李耀武;李紅;;苯妥英鈉對大鼠動脈粥樣硬化形成及發(fā)展的影響[J];中國實驗診斷學(xué);2008年11期

10 趙娟;李相軍;孫波;高海成;任立群;;維生素D_3聯(lián)合高脂飼料建立大鼠動脈粥樣硬化模型[J];實用醫(yī)學(xué)雜志;2009年21期

相關(guān)會議論文 前10條

1 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號：2477149