Objective: To observe MET-associated alteration during the trans-differentiation from MSCs to neuron-like cells, and to explore the possible molecular mechanism. Methods: Bone marrow MSCs were isolated from rat femur and purified in continuous cell culture. After induced differentiation to neuron-like cells by the combination of butylated hydroxyanisole(BHA)and dimethyl sulfoxide(DMSO), cells were tested by comparative polymerase chain reaction(PCR) for the relative expression of MET biomarkers ...

【文章頁數(shù)】：5 頁

【文章目錄】：
Introduction
Materials and Methods
    Materials
    MSC isolation and purification
    MSC induction to neuron
    Gene expression analysis by comparative PCR
    Cell cycle analysis by flow cytometry
    Statistical analysis
Results
    Cell morphology change when MSCs were induced to neurons
    EMT-associated genes were changed in the MSCderived neuron
    Cell proliferation property was changed in MSCderived neuron
    Wnt/β-catenin pathway was involved in EMT
Discussion



本文編號：3979068