軟腦膜黑色素瘤(LMM),從顱外黑色素瘤的轉(zhuǎn)移灶或從本有的黑色素細(xì)胞開始發(fā)展,在預(yù)后差的黑色素瘤患者中,有22%的發(fā)病率。在本實(shí)驗(yàn)研究中,我們建立了一個(gè)小鼠大腦LMM模型,在C57BL/6小鼠的頭骨上打孔,使用一個(gè)自我改良的微量注射器將B16細(xì)胞直接注入到蛛網(wǎng)膜下腔。35%至40%的小鼠大腦或大腦和小腦延髓池浸潤黑色素瘤,.10%的小鼠大腦或小腦浸潤,15%的小鼠枕大池和脊髓浸潤。黑色素瘤在大腦半球的表面形成了一個(gè)約1.5×1.3×1.0厘米大小的黑色膠狀團(tuán)塊,有多處隆起并聚集的小瘤塊。在腦基底部的柔腦膜、大腦和脊髓無可見黑色素瘤。組織病理學(xué)檢測可見黑色素瘤組織充滿B16細(xì)胞且血管生長迅速,腦組織無黑色素瘤細(xì)胞,黑色素細(xì)胞廣泛地滲透在軟腦膜層。在臨床上,在小鼠身上發(fā)現(xiàn)的腫瘤位置和可檢測的癥狀的相關(guān)性,在患者LMM是同樣適用的。為了測試的小鼠模型的可行性,我們也嘗試了用腫瘤細(xì)胞B16裂解物(TCL)免疫LMM模型小鼠去誘導(dǎo)抗LMM免疫。

【文章頁數(shù)】：65 頁

【學(xué)位級別】：博士

【文章目錄】：
Abstract
摘要
Chapter 1 Introduction
Chapter 2 Literature Review
    2.1 Melanoma
    2.2 Leptomeningeal melanoma (LMM)
    2.3 Anatomy (Topography of the Meninges)
    2.4 Previous Mouse model studies
Chapter 3 Materials, Methods and Results
    3.1 Mice
    3.2 Cell line culture
    3.3 Modification of Hamilton's syringe
    3.4 B16 cell inoculation
        3.4.a Tools and chemicals
        3.4.b Method
    3.5 Macroscopic analysis
    3.6 Histological analysis
    3.7 Preparation of fresh tumor cell lysate
    3.8 SDS-PAGE analysis of TCL
    3.9 Immunization
    3.10 The Results
        3.10.a Modification of the syringe for inoculating tumor cells
        3.10.b Establishment of a leptomeningeal melanoma mouse model
        3.10.C Evaluation of the immunogenicity of tumor cell lysate in mice inoculated with B16 melanoma cells in its subarachnoid space
Chapter 4 Discussion
References
Annexes
    Figures and tables
    Curriculum vitae
Acknowledgement



本文編號：3841658