背景:神經(jīng)軸突導(dǎo)向因子1作為血管內(nèi)皮細(xì)胞的促有絲分裂原,能夠促進(jìn)血管內(nèi)皮細(xì)胞的遷移和增殖,發(fā)揮誘導(dǎo)血管新生的作用。目的:觀察神經(jīng)軸突導(dǎo)向因子1裸質(zhì)粒轉(zhuǎn)染對(duì)5/6腎切除大鼠模型殘余腎功能的保護(hù)及對(duì)腎小管周圍毛細(xì)血管網(wǎng)的影響。方法:將30只SD大鼠隨機(jī)等分為假手術(shù)組、模型組和治療組。模型組和治療組大鼠切除左側(cè)腎臟上、下極各1/3,1周后切除右腎,建立殘腎模型。模型組和治療組大鼠于切除右腎的同時(shí)在左腎靜脈分別注射空質(zhì)粒IRES2-EGFP和pC MV6-XL5-Netrin-1-IRES2-EGFP。結(jié)果與結(jié)論:與模型組相比,治療組大鼠血尿素氮、血肌酐水平降低,腎間質(zhì)纖維化程度減輕,腎小管周圍毛細(xì)血管網(wǎng)的密度增加,腎小管胞漿中神經(jīng)軸突導(dǎo)向因子1蛋白表達(dá)增加。提示神經(jīng)軸突導(dǎo)向因子1裸質(zhì)粒轉(zhuǎn)染能明顯改善5/6腎切除模型大鼠的腎功能,減輕殘腎組織的病理?yè)p害和腎間質(zhì)纖維化面積,增加腎小管周圍毛細(xì)血管網(wǎng)密度、降低缺氧誘導(dǎo)因子1α表達(dá),從而改善腎間質(zhì)小管缺氧狀態(tài)。 

【文章來(lái)源】：中國(guó)組織工程研究. 2015,19(24)北大核心

【文章頁(yè)數(shù)】：8 頁(yè)

【文章目錄】：
INTRODUCTION
MATERIALS AND METHODS
    Design
    Time and setting
    Materials
    Methods
        Preparation of p CMV-NETRIN-1-IRES2-EGFP plasmids
        Establishment of 5/6 nephrectomized rat models and grouping
        Gene transfection
        Sample harvesting
        Morphological examination of the renal tissues
        Immunohistochemistry staining
        Western blot determination
        Main outcome measurements
        Statistical analysis
RESULTS
    Quantitative analysis of experimental animals
    Transfection effectiveness of p CMV-NETRIN-1-IRES2-EGFP
    Renal function and 24-hour urine protein
    Pathological changes of the rat kidney
    Peritubular capillary network density
    Expression of netrin-1 and hypoxia inducible factor 1αin the renal tissues of rats
DISCUSSION


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