【摘要】：通過(guò)設(shè)計(jì)1對(duì)fimA全基因通用引物,對(duì)已知不同人、動(dòng)物源的17株大腸埃希菌菌株的Ⅰ型菌毛fimA基因進(jìn)行PCR擴(kuò)增、克隆和測(cè)序,并與GenBank中9株菌株序列進(jìn)行同源性比較分析,探討I型菌毛宿主嗜性。結(jié)果表明:17株菌株均能擴(kuò)增出約549bp的預(yù)期大小目的片段�？寺『托蛄蟹治鲲@示,26株不同來(lái)源的大腸埃希菌fimA基因的核苷酸同源性為88.7%~100%,推導(dǎo)氨基酸同源性為88%~100%,其中出現(xiàn)差異的31個(gè)易發(fā)生突變的位點(diǎn)多為中性氨基酸,其中10株菌的fimA序列于第26個(gè)氨基酸位置連續(xù)插入了脯氨酸和蘇氨酸,且80%為豬源大腸埃希菌,因此提示I型菌毛在大腸埃希菌的進(jìn)化過(guò)程中可能具備一定的宿主嗜性。
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圖片說(shuō)明：圖１不同大腸埃希菌菌株ｆｉｍＡ基因ＰＣＲ擴(kuò)增結(jié)果Ｆｉｇ．１ＤｉｆｆｅｒｅｎｔＥ．ｃｏｌｉｓｔｒａｉｎｓｆｉｍＡｇｅｎｅ
[Abstract]:By designing a pair of universal primers for fimA gene, the fimA gene of type I fimbriae of 17 strains of Escherichia coli from different humans and animals was amplified, cloned and sequenced by PCR, and the homology with the sequence of 9 strains in GenBank was compared with that of 9 strains in GenBank to explore the host tropism of type I fimbriae. The results showed that the expected size and target fragments of about 549bp could be amplified by 17 strains. Cloning and sequence analysis showed that the nucleotide homology of fimA gene of 26 strains of Escherichia coli from different sources was 88.7% 鈮，

本文編號(hào)：2510924