【摘要】： 目的:觀察轉(zhuǎn)染BMP-2和VEGF165雙基因共表達(dá)質(zhì)粒在小鼠骨髓基質(zhì)干細(xì)胞的表達(dá)。方法:脂質(zhì)體介導(dǎo)下將雙基因真核表達(dá)質(zhì)粒pIRES-BMP2-VEGF165導(dǎo)入小鼠骨髓基質(zhì)干細(xì)胞,用RT-PCR和免疫組織化學(xué)方法觀察BMP-2和VEGF165雙基因在小鼠骨髓基質(zhì)干細(xì)胞內(nèi)的表達(dá)。結(jié)果:①傳代后的mBMSC_S增殖迅速,5-7 d細(xì)胞達(dá)90%融合。傳代后不再以集落方式生長(zhǎng),呈均勻分布生長(zhǎng),細(xì)胞形態(tài)均勻,與成纖維細(xì)胞形態(tài)相似,大部分呈長(zhǎng)梭形,排列規(guī)則。②轉(zhuǎn)染BMP-2、VEGF165的小鼠骨髓基質(zhì)干細(xì)胞有明顯的BMP-2和VEGF165 mRNA及其蛋白表達(dá)。結(jié)論:經(jīng)轉(zhuǎn)染BMP-2和VEGF165雙基因質(zhì)粒的小鼠骨髓基質(zhì)干細(xì)胞能同時(shí)表達(dá)以上兩種基因。
[Abstract]:Aim: to observe the expression of BMP-2 and VEGF165 co-expression plasmid in mouse bone marrow stem cells (BMSCs). Methods: the double gene eukaryotic expression plasmid pIRES-BMP2-VEGF165 was introduced into mouse bone marrow stroma stem cells mediated by liposomes. The expression of BMP-2 and VEGF165 genes in mouse bone marrow stroma stem cells was observed by RT-PCR and immunohistochemistry. Results: 1 the mBMSC_S proliferated rapidly after passage, and 90% of the cells were fused on the 7th day. After passage, it no longer grows in the form of colony, showing uniform distribution and growth, and the morphology of cells is similar to that of fibroblasts, most of which are long fusiform and regular arrangement. 2 the expression of BMP-2, VEGF165 mRNA and their proteins were obvious in mouse bone marrow stroma stem cells transfected with BMP-2,VEGF165. Conclusion: mouse bone marrow stroma stem cells transfected with BMP-2 and VEGF165 double gene plasmid can express the above two genes at the same time.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類(lèi)號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 孫強(qiáng),邱勇;BMPs信號(hào)通路調(diào)控骨髓間質(zhì)干細(xì)胞的成骨分化[J];臨床骨科雜志;2005年05期

2 孫立;田曉濱;張宇坤;郜勇;楊述華;;人骨形態(tài)發(fā)生蛋白2與血管內(nèi)皮生長(zhǎng)因子165雙基因真核表達(dá)質(zhì)粒的構(gòu)建及體外表達(dá)[J];中國(guó)臨床康復(fù);2006年09期

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本文編號(hào)：2510347