【摘要】：小細(xì)胞網(wǎng)狀核(PCRt)具有調(diào)節(jié)呼吸、發(fā)聲、咀嚼、吞咽等口面部運(yùn)動(dòng)的作用;還接受中樞神經(jīng)系統(tǒng)的投射和外周感覺信號(hào)的傳入,中繼本體感覺信息誘發(fā)口面部運(yùn)動(dòng)神經(jīng)元的反射性活動(dòng)。近年來對痙攣性腦癱患兒進(jìn)行選擇性脊神經(jīng)后根切斷術(shù)后發(fā)現(xiàn),患者四肢的肌張力不僅有大幅度下降,伴隨的流涎癥狀也有不同程度的改善,提示PCRt與控制吞咽和腺體分泌的腦干內(nèi)腦神經(jīng)核有神經(jīng)纖維聯(lián)系,但目前國內(nèi)對此方面的研究資料較少,本實(shí)驗(yàn)主要從形態(tài)學(xué)上觀察該核團(tuán)與直接調(diào)控吞咽和腺體分泌活動(dòng)的腦神經(jīng)核團(tuán)間的神經(jīng)聯(lián)系,以及進(jìn)一步了解網(wǎng)狀結(jié)構(gòu)中PCRt到脊髓頸段神經(jīng)元的神經(jīng)纖維投射。 方法 1.將碳素墨水微量注射注入一側(cè)PCRt,進(jìn)行HE染色,觀察PCRt的定位是否準(zhǔn)確。 2.將麥芽凝集素-辣根過氧化物酶(WGA-HRP)注入一側(cè)PCRt,采用TMB-ST法成色,在光學(xué)顯微鏡下觀察注射部位是否準(zhǔn)確及標(biāo)記情況。 3.將1,1′-雙十八烷-3,3,3′,3′-四甲基吲哚羧花青-高氯酸鹽(DiI)微量注射入一側(cè)PCRt,取材,熒光顯微鏡下觀察注射部位及標(biāo)記情況。 結(jié)果 1.舌下神經(jīng)核、迷走神經(jīng)背核、面神經(jīng)核、疑核、上泌涎核、下泌涎核、三叉神經(jīng)脊束核、三叉神經(jīng)運(yùn)動(dòng)核,均見有順行標(biāo)記纖維和終末,為對側(cè)優(yōu)勢;孤束核、前庭內(nèi)側(cè)核見有逆行標(biāo)記細(xì)胞及順行神經(jīng)纖維終末,對側(cè)多于同側(cè)。 2.頸段脊髓灰質(zhì),可在頸段脊髓灰質(zhì)Ⅰ、Ⅱ、Ⅲ板層發(fā)現(xiàn)順行標(biāo)記神經(jīng)纖維,在Ⅶ、Ⅷ、Ⅺ板層發(fā)現(xiàn)有少量逆行標(biāo)記細(xì)胞及順行標(biāo)記神經(jīng)纖維。 3.WGA-HRP和Dil標(biāo)記的結(jié)果分布相似,不同的是,DiI在上泌涎核未顯示出標(biāo)記,且在其他核團(tuán)標(biāo)記的細(xì)胞數(shù)目及神經(jīng)纖維終末沒有WGA-HRP標(biāo)記的多。 結(jié)論 1.通過對WGA-HRP、DiI示蹤的檢測,說明以小細(xì)胞網(wǎng)狀核為主的延髓網(wǎng)狀結(jié)構(gòu)易化區(qū)和大多數(shù)腦干內(nèi)腦神經(jīng)核、頸段脊髓神經(jīng)元之間存在直接的纖維聯(lián)系。 2.從小細(xì)胞網(wǎng)狀核到脊髓的投射纖維多經(jīng)由頸段脊髓灰質(zhì)的Ⅰ、Ⅱ、Ⅲ板層。而頸段脊髓灰質(zhì)Ⅶ、Ⅷ、Ⅺ板層與小細(xì)胞網(wǎng)狀核有雙向神經(jīng)纖維投射。
[Abstract]:Small cell reticular nucleus (PCRt) can regulate oral and facial movement such as respiration, voice, chewing, swallowing and so on. It also receives the projection of central nervous system and the afferent of peripheral sensory signal, and relays the sensory information of body to induce the reflexive activity of oral and facial motor neurons. In recent years, after selective posterior spinal nerve transection in children with spastic cerebral palsy, it was found that the muscle tension of the extremities was not only greatly decreased, but also the symptoms of salivation were improved in varying degrees, suggesting that PCRt is related to the nucleus of the brain nerve in the brain stem, which controls swallowing and gland secretion, but there is little research data in this field at present. The aim of this study was to observe the neural relationship between the nucleus and the nucleus directly regulating swallowing and gland secretion, and to further understand the nerve fiber projection from PCRt to the cervical neurons of the spinal cord in the reticular structure. Method 1. Carbon ink was injected into one side of PCRt, for HE staining to observe whether the location of PCRt was accurate. two銆，

本文編號(hào)：2508335