慢性腎功能不全患者和腎移植受者外周血調(diào)節(jié)性T細(xì)胞的表達(dá)
發(fā)布時(shí)間：2019-06-19 20:14
【摘要】： 調(diào)節(jié)性T細(xì)胞是一種免疫調(diào)節(jié)細(xì)胞,其主要以主動(dòng)方式誘導(dǎo)和維持機(jī)體外周免疫耐受,調(diào)節(jié)機(jī)體免疫平衡,多項(xiàng)研究發(fā)現(xiàn)其在預(yù)防和抑制自身免疫性疾病、炎癥、變態(tài)反應(yīng)和移植排斥中有著非常重要的作用。 慢性腎功能衰竭的發(fā)展過程可以隨著腎臟受損的逐步加重而分為下列四個(gè)時(shí)期:代償期、失代償期、衰竭期和尿毒癥期。慢性腎功能不全常并發(fā)免疫功能障礙,而且以細(xì)胞免疫異常為主。調(diào)節(jié)性T細(xì)胞(Treg)是具有免疫抑制功能的一群T細(xì)胞,在維持機(jī)體免疫自穩(wěn)、免疫耐受和防止自身免疫性疾病的發(fā)生方面具有十分重要的作用。我們利用流式細(xì)胞術(shù)檢測(cè)了50例慢性腎功能不全患者外周血CD4+CD127-Treg、CD4+ CD25+CD127- Treg的表達(dá)水平,并與健康人群作對(duì)比,以探討其在不同腎功能狀態(tài)下的變化。研究發(fā)現(xiàn)慢性腎功能不全各組患者外周血CD4+CD127-Treg占CD4+T細(xì)胞的比例顯著低于健康對(duì)照組,而CD4+CD25+CD127-Treg占CD4+T細(xì)胞的比例較健康對(duì)照組無顯著性差異,代償期患者CD4+T占淋巴細(xì)胞的比例跟健康對(duì)照組相比,沒有統(tǒng)計(jì)學(xué)差異,其余各期均顯著高于健康對(duì)照組。提示慢性腎功能不全患者存在免疫功能異常�；颊逤D4+T占淋巴細(xì)胞的比例,CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T細(xì)胞的比例與血清BUN和Cr值之間沒有相關(guān)性。 我們通過測(cè)定13例維持性血液透析患者血透前、血透后外周血CD4+T細(xì)胞占淋巴細(xì)胞的比例及CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T細(xì)胞的比例,探討血液透析前后患者體內(nèi)CD4+CD127-Treg、CD4+CD25+CD127-Treg的表達(dá)水平及意義。觀察發(fā)現(xiàn)維持性血液透析患者血透前CD4+T細(xì)胞占淋巴細(xì)胞的比例高于健康對(duì)照組,CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T細(xì)胞的比例低于健康對(duì)照組,血透后CD4+T的比例下降,CD4+CD25+CD127-Treg的比例有所上升。提示維持性血液透析患者外周血CD4+T數(shù)量增高,CD4+CD127-Treg、CD4+CD25+CD127-Treg數(shù)量降低,免疫功能紊亂,而血透有利于改善機(jī)體的免疫功能。 腎移植是目前尿毒癥患者理想、經(jīng)濟(jì),并可明顯提高生活質(zhì)量的一種治療方法,但是排斥反應(yīng)與長(zhǎng)期應(yīng)用免疫抑制劑的副作用,包括免疫抑制引起的感染、腫瘤發(fā)生率的增加等嚴(yán)重影響著移植物及受者的存活。如何控制器官移植后排斥反應(yīng),誘發(fā)宿主對(duì)移植物的免疫耐受,一直是移植免疫學(xué)領(lǐng)域研究的熱點(diǎn)及難題。在體內(nèi)外通過抗原非特異性或特異性擴(kuò)增,下調(diào)效應(yīng)性免疫細(xì)胞的功能并削弱其增殖能力,來抑制或控制機(jī)體免疫應(yīng)答的程度。我們將腎移植受者分為慢性排斥組和穩(wěn)定組,通過測(cè)定其外周血CD4+CD127-Treg、CD4+CD25+CD127-Treg水平,并與健康對(duì)照組作對(duì)比,探討其在誘導(dǎo)異源器官移植耐受方面的重要作用,為克服排斥反應(yīng)提供一些參考。 研究發(fā)現(xiàn)慢性排斥組淋巴細(xì)胞中CD4+T的比例明顯高于健康對(duì)照組和穩(wěn)定組,有統(tǒng)計(jì)學(xué)差異。而穩(wěn)定組CD4+T細(xì)胞的比例低于慢排組,與健康對(duì)照組相比無顯著差異。慢性排斥組CD4+CD127-Treg占CD4+T細(xì)胞的比例明顯低于健康對(duì)照組和穩(wěn)定組,有統(tǒng)計(jì)學(xué)差異;穩(wěn)定組CD4+CD127-Treg占CD4+T細(xì)胞的比例較健康對(duì)照組稍低但無統(tǒng)計(jì)學(xué)差異。慢性排斥組CD4+CD25+CD127-Treg占CD4+T細(xì)胞的比例低于健康對(duì)照組且有統(tǒng)計(jì)學(xué)差異,與穩(wěn)定組相比無統(tǒng)計(jì)學(xué)差異。穩(wěn)定組CD4+CD25+CD127-Treg占CD4+T細(xì)胞的比例,與健康對(duì)照組相比無統(tǒng)計(jì)學(xué)差異。提示如果促進(jìn)Treg的發(fā)育和增殖,將能有效地增強(qiáng)機(jī)體的免疫耐受,降低免疫抑制劑的用量。
[Abstract]:The regulatory T cell is an immunomodulatory cell, which mainly induces and maintains the peripheral immune tolerance of the body in an active manner, regulates the immune balance of the body, and has been found to be used for preventing and inhibiting the autoimmune diseases and inflammation, Allergy and transplant rejection have a very important role. The development of chronic renal failure can be divided into the following four periods with the gradual increase of the kidney damage: the compensation period, the decompensation stage, the failure period and the urinary toxicity. The chronic renal function is not always accompanied with immune dysfunction, and is abnormal in cellular immunity. The primary regulatory T cell (Treg) is a group of T cells with immunosuppressive function, which is of great importance in the maintenance of immune homeostasis, immune tolerance and the prevention of autoimmune diseases. The expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in the peripheral blood of 50 patients with chronic renal insufficiency was detected by flow cytometry and compared with the healthy population to investigate the expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in 50 patients with chronic renal insufficiency. The results showed that the ratio of CD4 + CD127-Treg in the peripheral blood of the patients with chronic renal insufficiency was significantly lower than that in the healthy control group, while the proportion of CD4 + CD25 + CD127-Treg in the CD4 + T cells was significantly lower than that in the healthy control group. The difference is that the rest of the period is significantly higher than that of the healthy pair Group. It is suggested that the immune function in patients with chronic renal insufficiency Abnormality. The ratio of CD4 + T in the patient to the lymphocytes, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg, or CD4 + T cells, is not associated with the serum BUN and Cr values The expression of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in patients before and after hemodialysis was discussed by measuring the ratio of CD4 + T cells in peripheral blood and CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells before and after hemodialysis. The results showed that the ratio of CD4 + T cells to lymphocytes in the patients with maintenance hemodialysis was higher than that of healthy control group, and the ratio of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells was lower than that of healthy control group. The ratio of CD4 + T after hemodialysis was lower, and the ratio of CD4 + CD25 + CD127-Treg was lower. The number of CD4 + T, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of patients with maintenance hemodialysis were increased, and the number of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg decreased and the immune function was disordered. Kidney transplantation is an ideal and economical method for uremic patients and can obviously improve the quality of life, but the side effects of rejection and long-term application of immunosuppressants, including the maintenance-free The infection, the increase of the incidence of the tumor, etc. caused by the inhibition of the epidemic are seriously affected by the shift. The survival of the plant and the recipient. How to control the rejection after organ transplantation and to induce the immune tolerance of the host to the graft has been the field of immunology It is a hot topic and a difficult problem to study. In vivo, the function of effector immune cells is down-regulated and its proliferation ability is reduced by non-specific or specific amplification of the antigen, so as to inhibit or control the proliferation of the immune cells. The level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of the renal transplant recipients was measured and compared with the healthy control group. The results showed that the ratio of CD4 + T in the lymphocytes of the chronic rejection group was significantly higher than that of the healthy control group. and the proportion of the stable group CD4 + T cells is lower than that of the slow-row group and the stable group, Compared with the control group, the proportion of CD4 + CD127-Treg in the chronic rejection group was significantly lower than that in the healthy control group and the stable group, and the proportion of the CD4 + CD127-Treg in the stable group was significantly lower than that of the healthy control group and the stable group, and the ratio of the CD4 + CD127-Treg in the stable group to the CD4 + T cell was healthy. The proportion of CD4 + CD25 + CD127-Treg in chronic rejection group was lower than that of healthy control group and there was no statistical difference. The ratio of CD4 + CD25 + CD127-Treg in the stable group to CD4 + T cells and health There is no statistical difference in the control group. It is suggested that the immune tolerance of the body can be effectively enhanced if the development and proliferation of the Treg are promoted.
【學(xué)位授予單位】：廣西師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R392.12;R692.5;R699
本文編號(hào)：2502610
[Abstract]:The regulatory T cell is an immunomodulatory cell, which mainly induces and maintains the peripheral immune tolerance of the body in an active manner, regulates the immune balance of the body, and has been found to be used for preventing and inhibiting the autoimmune diseases and inflammation, Allergy and transplant rejection have a very important role. The development of chronic renal failure can be divided into the following four periods with the gradual increase of the kidney damage: the compensation period, the decompensation stage, the failure period and the urinary toxicity. The chronic renal function is not always accompanied with immune dysfunction, and is abnormal in cellular immunity. The primary regulatory T cell (Treg) is a group of T cells with immunosuppressive function, which is of great importance in the maintenance of immune homeostasis, immune tolerance and the prevention of autoimmune diseases. The expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in the peripheral blood of 50 patients with chronic renal insufficiency was detected by flow cytometry and compared with the healthy population to investigate the expression level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in 50 patients with chronic renal insufficiency. The results showed that the ratio of CD4 + CD127-Treg in the peripheral blood of the patients with chronic renal insufficiency was significantly lower than that in the healthy control group, while the proportion of CD4 + CD25 + CD127-Treg in the CD4 + T cells was significantly lower than that in the healthy control group. The difference is that the rest of the period is significantly higher than that of the healthy pair Group. It is suggested that the immune function in patients with chronic renal insufficiency Abnormality. The ratio of CD4 + T in the patient to the lymphocytes, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg, or CD4 + T cells, is not associated with the serum BUN and Cr values The expression of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in patients before and after hemodialysis was discussed by measuring the ratio of CD4 + T cells in peripheral blood and CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells before and after hemodialysis. The results showed that the ratio of CD4 + T cells to lymphocytes in the patients with maintenance hemodialysis was higher than that of healthy control group, and the ratio of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg to CD4 + T cells was lower than that of healthy control group. The ratio of CD4 + T after hemodialysis was lower, and the ratio of CD4 + CD25 + CD127-Treg was lower. The number of CD4 + T, CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of patients with maintenance hemodialysis were increased, and the number of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg decreased and the immune function was disordered. Kidney transplantation is an ideal and economical method for uremic patients and can obviously improve the quality of life, but the side effects of rejection and long-term application of immunosuppressants, including the maintenance-free The infection, the increase of the incidence of the tumor, etc. caused by the inhibition of the epidemic are seriously affected by the shift. The survival of the plant and the recipient. How to control the rejection after organ transplantation and to induce the immune tolerance of the host to the graft has been the field of immunology It is a hot topic and a difficult problem to study. In vivo, the function of effector immune cells is down-regulated and its proliferation ability is reduced by non-specific or specific amplification of the antigen, so as to inhibit or control the proliferation of the immune cells. The level of CD4 + CD127-Treg, CD4 + CD25 + CD127-Treg in peripheral blood of the renal transplant recipients was measured and compared with the healthy control group. The results showed that the ratio of CD4 + T in the lymphocytes of the chronic rejection group was significantly higher than that of the healthy control group. and the proportion of the stable group CD4 + T cells is lower than that of the slow-row group and the stable group, Compared with the control group, the proportion of CD4 + CD127-Treg in the chronic rejection group was significantly lower than that in the healthy control group and the stable group, and the proportion of the CD4 + CD127-Treg in the stable group was significantly lower than that of the healthy control group and the stable group, and the ratio of the CD4 + CD127-Treg in the stable group to the CD4 + T cell was healthy. The proportion of CD4 + CD25 + CD127-Treg in chronic rejection group was lower than that of healthy control group and there was no statistical difference. The ratio of CD4 + CD25 + CD127-Treg in the stable group to CD4 + T cells and health There is no statistical difference in the control group. It is suggested that the immune tolerance of the body can be effectively enhanced if the development and proliferation of the Treg are promoted.
【學(xué)位授予單位】：廣西師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R392.12;R692.5;R699
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 雷樹勇,謝國(guó)乾,黃承樂;血透患者細(xì)胞免疫功能的變化[J];武警醫(yī)學(xué)院學(xué)報(bào);2002年02期
，本文編號(hào)：2502610
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