【摘要】： 目的: 比較外科手術(shù)與藥物誘導(dǎo)建立大鼠急性肝衰竭(ALF)模型的方法,探討一種制備穩(wěn)定的ALF模型的方法。建立與人急性肝衰竭病理過程、生化改變相似,實(shí)用性、重復(fù)性好的動(dòng)物模型,為研究急性肝衰竭發(fā)生的病理生理機(jī)制、藥物篩選及療效評價(jià)提供適宜的模型。 方法: 將SD大鼠60只隨機(jī)分為二組: (1)手術(shù)切除誘導(dǎo)組(A組),切除約85 %肝臟組織,術(shù)中經(jīng)左腎靜脈注入5%葡萄糖氯化鈉溶液10ml/kg體重;(2)藥物誘導(dǎo)組(B組),將四氯化碳(CCl4)與等量花生油混合成50%溶液,按0.9ml/ kg腹腔注射。觀察手術(shù)死亡率、模型建立后2 4 h大鼠存活率、血谷丙轉(zhuǎn)氨酶(ALT)、天門冬氨酸氨基轉(zhuǎn)移酶(AST)、白蛋白(ALB)、堿性磷酸酶(ALP)、血氨(NH3)、總膽紅素(TBIL)、凝血酶原時(shí)間(PT)和血糖(BG)等指標(biāo)。并取不同時(shí)間點(diǎn)的肝臟組織行病理學(xué)檢查。 結(jié)果: 手術(shù)切除組與藥物組的大鼠建模成功后24h存活率分別為0%,30%,手術(shù)切除組ALT、AST、ALP、NH3、PT的水平要顯著高于藥物組(P0.05),而TBIL、ALB和BG水平低于藥物誘導(dǎo)組,但差異無顯著性(P0.05)。A組12h病理顯示肝臟損害不嚴(yán)重;B組72h病理顯示肝臟大片壞死,損害嚴(yán)重。 結(jié)論: 通過改進(jìn)的85%肝切除術(shù)可建立較理想的大鼠ALF模型,術(shù)中經(jīng)左腎靜脈補(bǔ)液可減少手術(shù)死亡率。采用手術(shù)切除法制備的ALF模型值得推廣應(yīng)用,具有快速、簡便、易控的特點(diǎn),可以用于更深一步的研究和應(yīng)用。
[Abstract]:Objective: to compare the methods of establishing (ALF) model of acute liver failure in rats by surgery and drug induction, and to explore a method of establishing stable ALF model. An animal model similar to the pathological process and biochemical changes of human acute liver failure was established, which provided a suitable model for the study of pathophysiological mechanism, drug screening and curative effect evaluation of acute liver failure. Methods: 60 SD rats were randomly divided into two groups: (1) resection induction group (group A), resection of about 85% liver tissue, intraoperative injection of 5% glucose sodium chloride solution 10ml/kg body weight through left renal vein; (2) in the drug induction group (group B), carbon tetrachloride (CCl4) was mixed with the same amount of peanut oil into 50% solution and injected intraperitoneally according to 0.9ml/ kg. The survival rate of 24 hours after the establishment of the model was observed. (ALT), aspartate aminotransferase (AST), albumin (ALB), alkaline phosphatase (ALP), blood ammonia (NH3) and total bilirubin (TBIL), were observed. Prothrombin time (PT) and blood glucose (BG) were measured. The liver tissues at different time points were examined by pathology. Results: the 24 h survival rates of the rats in the resection group and the drug group were 0% and 30% respectively. The level of ALT,AST,ALP,NH3,PT in the resection group was significantly higher than that in the drug group (P 0.05), while TBIL, was significantly higher than that in the drug group. The levels of ALB and BG in the drug-induced group were lower than those in the drug-induced group, but there was no significant difference (P 0.05). 12 h pathology in the). A group showed that the liver damage was not serious. In group B, 72 h pathology showed massive necrosis of the liver and serious damage. Conclusion: an ideal rat model of ALF can be established by improved 85% hepatectomy, and intraoperative infusion of fluid through left renal vein can reduce the operative mortality. The ALF model prepared by surgical resection is worthy of popularization and application, which is rapid, simple and easy to control, and can be used for further research and application.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R575.3;R-332

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