【摘要】： 目的探討補(bǔ)充雙歧桿菌的保護(hù)作用是否與降低腸組織PAF和提高腸組織SIgA有關(guān),為應(yīng)用雙歧桿菌防治新生兒壞死性小腸結(jié)腸炎提供科學(xué)依據(jù)。 方法選用84只兩日齡新生SD大鼠,雌雄不限,隨機(jī)分三組:實驗組(A)、對照組(B)、空白對照組(C),每組n=28。A組:每只0.04g(含2億個活菌)雙歧桿菌灌胃(用PH7.4的生理鹽水配制0.2ml/只),持續(xù)一周,每天一次;B和C組:每只0.2ml生理鹽水灌胃持續(xù)一周,每天一次。實驗組和對照組一周后,均注射LPS(5mg/kg,用生理鹽水配成5mg/ml)建立壞死性小腸結(jié)腸炎模型。三組于A組和B組腹腔注射LPS后,2、6、12、24小時,每個時間點隨機(jī)抽取7只采用斷頸法處死,解剖留取十二指腸下端至直腸上端腸道組織,其中回盲部近端腸管觀察SD鼠病理標(biāo)本,剩余的腸管ELISA檢測腸組織勻漿中PAF、SIgA含量。采用SPSS16.0統(tǒng)計軟件,計量資料結(jié)果以均值±標(biāo)準(zhǔn)差(ˉX±SD)表示,組間比較采用t檢驗法,P0.05提示差異有顯著統(tǒng)計學(xué)意義。 結(jié)果 1.各實驗組新生SD大鼠腸組織病理形態(tài)學(xué)改變 新生SD大鼠注射LPS后,B組中新生SD大鼠出現(xiàn)懶動、萎靡,1-2小時出現(xiàn)腹脹、腹瀉,6-12小時上述癥狀加重,同時出現(xiàn)腹式呼吸增快,口唇青紫,少動,體毛凌亂、少光澤,嚴(yán)重者抽搐,四肢青紫,周身涼,甚至死亡;動物處死后,見胃儲留明顯,腸壁及腸系膜充血,腸管擴(kuò)張、水腫,腸腔滲出物增多,嚴(yán)重者見腸壁點、片狀出血壞死,24小時存活者上述癥狀緩解。H-E染色后光鏡下變化:新生SD大鼠注射LPS后,B組2小時腸上皮細(xì)胞變性,絨毛萎縮;6小時可見小腸上皮結(jié)構(gòu)完全消失,絨毛腺體脫落、缺失,固有層毛細(xì)血管充血,炎細(xì)胞浸潤;12小時部分腸絨毛上皮細(xì)胞開始修復(fù),但絨毛結(jié)構(gòu)不清;24小時后腸絨毛結(jié)構(gòu)明顯。A組較B組相比,癥狀較輕。 2.各組新生SD大鼠腸組織PAF、SIgA含量變化 B組腸組織PAF的含量在2h、6h、12h與C組比較有顯著增加(P0.05),A組腸組織PAF的含量在2h、6h、12h與B組比較有顯著減少(P0.05)。A組腸組織SIgA的含量在2h、6h、12h與C組比較有顯著增加(P0.05),B組腸組織SIgA的含量在2h、6h、12h與A組比較有顯著減少(P0.05)。 結(jié)論 1.內(nèi)毒素引起新生鼠壞死小腸結(jié)腸炎模型中,外源性給予雙歧桿菌實驗組腸組織PAF的含量與對照組比較明顯降低,表明雙歧桿菌可能通過抑制炎癥級聯(lián)反應(yīng)中PAF表達(dá)而保護(hù)腸道,能減輕新生鼠壞死性小腸結(jié)腸炎模型腸組織病理改變。 2.內(nèi)毒素引起新生鼠壞死小腸結(jié)腸炎模型中,外源性給予雙歧桿菌實驗組腸組織SIgA的含量與對照組比較明顯增加,表明雙歧桿菌可能通過提高腸道免疫SIgA表達(dá)而保護(hù)腸道。
[Abstract]:Objective to investigate whether the protective effect of Bifidobacillus supplementation is related to the decrease of PAF in intestinal tissue and the increase of SIgA in intestinal tissue, and to provide scientific basis for the application of Bifidobacterium in the prevention and treatment of neonatal necrotizing enterocolitis. Methods 84 two-day-old neonatal SD rats, male and female, were randomly divided into three groups: experimental group (A), control group (B), blank control group (C), Group A: 0.04g Bifidobacterium (0.2ml/ only prepared with PH7.4 saline) was given intragastrically for one week, once a day. Group B and C: each 0.2ml saline was given intragastrically once a day. One week later, the experimental group and the control group were injected with LPS (5 mg 路kg ~ (- 1) and normal saline (5mg/ml) to establish the model of necrotizing enterocolitis. After intraabdominal injection of LPS in group A and group B, 2, 6, 12, 24 hours, 7 rats in each time point were randomly selected and killed by neck amputation, and the intestinal tissues from the lower end of the duodenum to the upper end of the rectum were dissected. The pathological specimens of SD mice were observed by proximal ileocecum, and the content of PAF,SIgA in intestinal homogenate was detected by residual intestinal ELISA. Using SPSS16.0 statistical software, the results of measurement data were expressed by mean 鹵standard deviation (鈮，

本文編號：2480509