發(fā)布時間：2019-03-29 08:15

【摘要】： IFN-γ(gamma干擾素)是-種常見的感染因子,是炎癥反應(yīng)重要的組成部分。在孕母懷胎期間,不可避免受到各種感染因子的影響,而這些感染因子是否對胎兒神經(jīng)系統(tǒng)的發(fā)育產(chǎn)生不良影響卻少有文獻報道。本文利用IFN-γ(濃度200U/ml)對大鼠體外培養(yǎng)胚胎16天的神經(jīng)干細胞進行刺激,研究作為炎癥反應(yīng)重要組分的IFN-γ對胚胎神經(jīng)干細胞的影響。 本文在第一部分首先建立了大鼠胚胎神經(jīng)干細胞體外培養(yǎng)的模型。我們分離大鼠胚齡16天的神經(jīng)干細胞,進行體外培養(yǎng)。這些神經(jīng)干細胞能夠表達特異性的標志nestin,當撤掉生長因子,加入血清時,能分化為神經(jīng)元、星形膠質(zhì)細胞和少突膠質(zhì)細胞。我們以神經(jīng)干細胞體外培養(yǎng)模型為基礎(chǔ)對感染因子作用于胚胎神經(jīng)干細胞的影響做出深入的分析研究。 在本文的第二部分,我們采用IFN-γ這一常見的感染因子,對大鼠的神經(jīng)干細胞進行刺激,觀察炎性分子對神經(jīng)干細胞生長、分化的影響。我們發(fā)現(xiàn),IFN-γ刺激2天能夠顯著上調(diào)神經(jīng)干細胞表面MHC分子的表達。同時,IFN-γ能夠促使神經(jīng)干細胞向神經(jīng)元和少突膠質(zhì)細胞的分化,而抑制向星形膠質(zhì)細胞的分化。 結(jié)論:我們成功建立了大鼠胚胎神經(jīng)干細胞體外培養(yǎng)的模型。這種方法得到的神經(jīng)干細胞90%以上表達神經(jīng)干細胞特異性標志nestin,同時具有多向分化能力,能夠分化成Rip陽性的少突膠質(zhì)細胞,β-tubulinⅢ陽性的神經(jīng)元和GFAP陽性的星形膠質(zhì)細胞。IFN-γ作為一種感染因子,在高劑量下對胚胎神經(jīng)干細胞具有毒性作用。IFN-γ能夠上調(diào)神經(jīng)干細胞MHCⅠ類和MHCⅡ類分子的表達,并且能夠促進神經(jīng)干細胞向神經(jīng)元和少突膠質(zhì)細胞的分化,降低向星形膠質(zhì)細胞的分化。
[Abstract]:IFN- 緯 (gamma interferon) is a common infectious factor and an important component of inflammatory response. During pregnancy, it is inevitable to be affected by a variety of infection factors, but whether these infection factors have an adverse effect on the development of the fetal nervous system is rarely reported in the literature. IFN- 緯 (concentration 200U/ml) was used to stimulate neural stem cells (NSCs) of rat embryos cultured in vitro for 16 days. The effects of IFN- 緯, an important component of inflammatory reaction, on embryonic neural stem cells (NSCs) were studied. In the first part, the culture model of rat embryonic neural stem cells in vitro was established. Neural stem cells (NSCs) aged 16 days were isolated and cultured in vitro. These neural stem cells can express specific markers, nestin, when removed from the growth factor, added to the serum, can differentiate into neurons, astrocytes and oligodendrocytes. We studied the effects of infection factors on embryonic neural stem cells based on neural stem cell culture model in vitro. In the second part of this paper, we used IFN- 緯, a common infection factor, to stimulate rat neural stem cells and observe the effects of inflammatory molecules on the growth and differentiation of neural stem cells. We found that IFN- 緯 stimulation for 2 days could significantly up-regulate the expression of MHC molecules on the surface of neural stem cells. At the same time, IFN- 緯 can promote the differentiation of neural stem cells into neurons and oligodendrocytes, but inhibit the differentiation of neural stem cells to astrocytes. Conclusion: in vitro culture model of rat embryonic neural stem cells has been successfully established. The neural stem cells obtained by this method express more than 90% of the neural stem cell-specific marker nestin, and have the ability to differentiate into Rip-positive oligodendrocytes. 尾-tubulin 鈪，

本文編號：2449331