【摘要】：目的研究骨形態(tài)發(fā)生蛋白-14(BMP-14)和堿性成纖維細胞生長因子(bFGF)聯(lián)合誘導(dǎo)大鼠骨髓間充質(zhì)干細胞(BMSC)向軟骨分化的協(xié)同作用。方法采用密度梯度離心法與貼壁分離法相結(jié)合分離、純化大鼠骨髓間充質(zhì)干細胞,用含10%胎牛血清的L-DMEM培養(yǎng)至第3代作為種子細胞,對照組A組不加生長因子,實驗組B、C、D組分別加入100ng/ml BMP-14、10ng/ml bFGF和100ng/ml BMP-14+10ng/ml bFGF,培養(yǎng)24h后各組間行細胞增殖比較,14d后觀察各組細胞形態(tài)學(xué)改變,各組間行Ⅱ型膠原免疫細胞化學(xué)染色和阿爾辛藍染色。結(jié)果培養(yǎng)14d后,A、B、C、D4組的Ⅱ型膠原細胞染色陽性率分別為(7.5210±3.7243)%、(40.8271±1.2787)%、(17.2818±1.4242)%、(60.5114±1.7634)%;阿爾辛藍染結(jié)果為:A組陰性,B、C組呈淡染,D組呈藍色。結(jié)論BMP-14和bFGF的聯(lián)合應(yīng)用較單個細胞因子可以明顯誘導(dǎo)骨髓間充質(zhì)干細胞向軟骨細胞表型分化及促進細胞增殖。
[Abstract]:Objective to study the synergistic effect of bone morphogenetic protein-14 (BMP-14) and basic fibroblast growth factor (bFGF) on the differentiation of rat bone marrow mesenchymal stem cells (BMSC) into cartilage. Methods Rat bone marrow mesenchymal stem cells were purified by density gradient centrifugation and adherent separation. L-DMEM containing 10% fetal bovine serum was used as seed cells for the third passage. After 24 hours of 100ng/ml BMP-14,10ng/ml bFGF and 100ng/ml BMP-14 10ng/ml bFGF, culture, the cell proliferation was compared in the experimental group, and the morphological changes of each group were observed after 14 days. Type 鈪，

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