FLJ22349基因功能的初步研究
發(fā)布時(shí)間：2018-12-28 21:04
【摘要】： 背景與目的:FLJ22349基因位于染色體22q13.2上,編碼一個(gè)229個(gè)氨基酸的蛋白。生物信息學(xué)分析發(fā)現(xiàn)多種腫瘤組織中有FLJ22349的表達(dá),預(yù)測該基因可能與腫瘤的發(fā)生發(fā)展有密切關(guān)系。本研究旨在用生物信息學(xué)方法分析FLJ22349的結(jié)構(gòu)和功能,并初步研究FLJ22349在HEK293及乳腺癌細(xì)胞中的作用。方法:運(yùn)用各種生物信息學(xué)數(shù)據(jù)庫初步分析FLJ22349的結(jié)構(gòu)和功能。運(yùn)用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)方法分析FLJ22349基因在小鼠正常組織,人腫瘤細(xì)胞株及人胚腎細(xì)胞系HEK293中的表達(dá)情況。將載有FLJ22349基因的質(zhì)粒轉(zhuǎn)染入HEK293,MCF-7,MDA-MB 231中,觀察該基因的亞細(xì)胞定位,運(yùn)用MTT方法,檢測細(xì)胞的生長變化。結(jié)果:FLJ22349基因在人類、猩猩、恒河猴、褐家鼠、小鼠和狗中有很高的同源性,但與其它已知的基因無同源性。小鼠正常組織中有FLJ22349表達(dá);乳腺癌細(xì)胞株MDA-MB 231,肝癌細(xì)胞株Bel-7402也有表達(dá);FLJ22349主要定位于細(xì)胞核;轉(zhuǎn)染FLJ22349的HEK293,MCF-7細(xì)胞株生長曲線觀察到促進(jìn)細(xì)胞生長(P＜0.05)。FLJ22349轉(zhuǎn)染120h后,MTT實(shí)驗(yàn)觀察到HEK293,MCF-7細(xì)胞的增殖率分別為299%,456%;而對照組分別為233%,272%。結(jié)論:FLJ22349基因過表達(dá)促進(jìn)HEK293和MCF-7細(xì)胞的生長。 [目的]綜合評價(jià)MDM2(Murine double minute 2)基因啟動(dòng)子309位點(diǎn)多態(tài)性與乳腺癌易感性的關(guān)系。[方法]檢索中國醫(yī)學(xué)文獻(xiàn)數(shù)據(jù)庫和PUBMED,以得到MDM2基因SNP309與乳腺癌易感性的關(guān)系的病例對照研究,并用meta分析的方法合并SNP309與乳腺癌易感性的關(guān)系的OR值。然后進(jìn)行其中有家族史的乳腺癌亞組分析,敏感性分析和文獻(xiàn)的發(fā)表偏倚檢驗(yàn)�！窘Y(jié)果]本次meta分析共納入10篇文獻(xiàn),乳腺癌家族史組有3篇;累計(jì)病例/535例,對照8272例,G等位基因相對于T等位基因OR值為1.01,可信區(qū)間0.96-1.06,沒有顯著性。乳腺癌家族史組G等位基因相對于T等位基因OR值為1.06,可信區(qū)間0.94-1.19,沒有顯著性。[結(jié)論]MDM2基因309T＞G多態(tài)與乳腺癌易感性沒有顯著性的關(guān)系。
[Abstract]:Background & AIM: FLJ22349 gene is located on chromosome 22q13.2 and encodes a 229 amino acid protein. Bioinformatics analysis showed that FLJ22349 was expressed in many kinds of tumor tissues, and it was predicted that this gene might be closely related to the occurrence and development of tumor. The purpose of this study was to analyze the structure and function of FLJ22349 by bioinformatics and to study the role of FLJ22349 in HEK293 and breast cancer cells. Methods: the structure and function of FLJ22349 were analyzed with various bioinformatics databases. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to analyze the expression of FLJ22349 gene in mouse normal tissue, human tumor cell line and human embryonic kidney cell line HEK293. The plasmid containing FLJ22349 gene was transfected into HEK293,MCF-7,MDA-MB 231. The subcellular localization of the gene was observed and the cell growth was detected by MTT method. Results: FLJ22349 gene had high homology in human, orangutan, rhesus monkey, Rattus norvegicus, mouse and dog, but had no homology with other known genes. FLJ22349 was expressed in normal tissues of mice, MDA-MB 231 in breast cancer cell line and Bel-7402 in hepatoma cell line, FLJ22349 was mainly located in nucleus. The growth curve of HEK293,MCF-7 cell line transfected with FLJ22349 was observed to promote cell growth (P < 0. 05). After 120 hours of FLJ22349 transfection, the proliferation rate of HEK293,MCF-7 cells was observed by MTT assay as 299cells and 456456cells, respectively. In the control group, 2333 and 272 respectively. Conclusion: overexpression of FLJ22349 gene promotes the growth of HEK293 and MCF-7 cells. [objective] to evaluate the association between MDM2 (Murine double minute 2) promoter 309 polymorphism and breast cancer susceptibility. [methods] A case-control study on the relationship between MDM2 gene SNP309 and breast cancer susceptibility was carried out by searching Chinese medical literature database and PUBMED, and the OR value of SNP309 and breast cancer susceptibility was combined with meta analysis. Then the family history of breast cancer subgroup analysis, sensitivity analysis and publication bias test. [results] the meta analysis included 10 articles, breast cancer family history group 3; The OR value of G allele to T allele was 1.01and the confidence interval was 0.96-1.06. there was no significant difference between G allele and T allele. The OR value of G allele to T allele in the family history group of breast cancer was 1.06 and the confidence interval was 0.94-1.19. There was no significant difference between G allele and T allele. [conclusion] MDM2 gene 309T > G polymorphism has no significant relationship with breast cancer susceptibility.
【學(xué)位授予單位】：中國協(xié)和醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R346
本文編號：2394434
[Abstract]:Background & AIM: FLJ22349 gene is located on chromosome 22q13.2 and encodes a 229 amino acid protein. Bioinformatics analysis showed that FLJ22349 was expressed in many kinds of tumor tissues, and it was predicted that this gene might be closely related to the occurrence and development of tumor. The purpose of this study was to analyze the structure and function of FLJ22349 by bioinformatics and to study the role of FLJ22349 in HEK293 and breast cancer cells. Methods: the structure and function of FLJ22349 were analyzed with various bioinformatics databases. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to analyze the expression of FLJ22349 gene in mouse normal tissue, human tumor cell line and human embryonic kidney cell line HEK293. The plasmid containing FLJ22349 gene was transfected into HEK293,MCF-7,MDA-MB 231. The subcellular localization of the gene was observed and the cell growth was detected by MTT method. Results: FLJ22349 gene had high homology in human, orangutan, rhesus monkey, Rattus norvegicus, mouse and dog, but had no homology with other known genes. FLJ22349 was expressed in normal tissues of mice, MDA-MB 231 in breast cancer cell line and Bel-7402 in hepatoma cell line, FLJ22349 was mainly located in nucleus. The growth curve of HEK293,MCF-7 cell line transfected with FLJ22349 was observed to promote cell growth (P < 0. 05). After 120 hours of FLJ22349 transfection, the proliferation rate of HEK293,MCF-7 cells was observed by MTT assay as 299cells and 456456cells, respectively. In the control group, 2333 and 272 respectively. Conclusion: overexpression of FLJ22349 gene promotes the growth of HEK293 and MCF-7 cells. [objective] to evaluate the association between MDM2 (Murine double minute 2) promoter 309 polymorphism and breast cancer susceptibility. [methods] A case-control study on the relationship between MDM2 gene SNP309 and breast cancer susceptibility was carried out by searching Chinese medical literature database and PUBMED, and the OR value of SNP309 and breast cancer susceptibility was combined with meta analysis. Then the family history of breast cancer subgroup analysis, sensitivity analysis and publication bias test. [results] the meta analysis included 10 articles, breast cancer family history group 3; The OR value of G allele to T allele was 1.01and the confidence interval was 0.96-1.06. there was no significant difference between G allele and T allele. The OR value of G allele to T allele in the family history group of breast cancer was 1.06 and the confidence interval was 0.94-1.19. There was no significant difference between G allele and T allele. [conclusion] MDM2 gene 309T > G polymorphism has no significant relationship with breast cancer susceptibility.
【學(xué)位授予單位】：中國協(xié)和醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R346
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相關(guān)期刊論文 前3條
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