【摘要】： 目的了解1800MHz電磁波出生前暴露對大鼠海馬NCAM和GABA受體表達的影響 方法7周齡SD雌性大鼠32只,雄性16只,適應(yīng)環(huán)境一周后以雌：雄=2：1交配,受孕后雌鼠隨機分為4組(兩個實驗組和兩個對照組)。實驗組采用1800MHz射頻電磁波對大鼠于孕0～20天進行21天連續(xù)暴露,每天12小時,功率密度為0.5 mW／cm2和1.0 mW／cm2,各實驗組分別設(shè)置虛擬暴露作為對照組。暴露期間的溫度、濕度、背景噪聲等環(huán)境條件穩(wěn)定。暴露完成后在F1代子鼠長至三周齡和七周齡,每組隨機抽取8只子鼠采用免疫組織化學(xué)方法分別對海馬GFAP、NCAM及GABA受體進行染色,然后采用圖像分析系統(tǒng)分析海馬CA1、CA3和DG區(qū)(齒狀回)的蛋白表達。 結(jié)果1.0mW／cm2暴露組未發(fā)現(xiàn)對三周齡、七周齡F1代大鼠海馬各區(qū)GFAP、NCAM、GABA受體表達有影響,其表達與對照組無統(tǒng)計學(xué)意義；0.5mW／cm2暴露組三周齡F1代大鼠海馬各區(qū)NCAM、GABA受體表達和七周齡GFAP、NCAM、GABA受體表達均出現(xiàn)了下調(diào),其表達與對照組相比有統(tǒng)計學(xué)意義。結(jié)論本實驗條件下,1800MHz功率密度0.5mW／cm2電磁輻射出生前暴露使三周齡大鼠NCAM與GABA受體和七周齡大鼠GFAP、NCAM與GABA受體在海馬各區(qū)表達下調(diào)。
[Abstract]:Objective to investigate the effects of 1800MHz electromagnetic wave exposure before birth on the expression of NCAM and GABA receptors in hippocampus of rats. Methods Thirty-two 7-week-old SD female rats and 16 male rats were used to mate with female: male = 2:1 after adapting to the environment for one week. After conception, female rats were randomly divided into 4 groups (two experimental groups and two control groups). Rats in the experimental group were exposed to 1800MHz radio frequency electromagnetic wave for 21 days, 12 hours a day, and the power density was 0.5 mW/cm2 and 1.0 mW/cm2, respectively. Temperature, humidity, background noise and other environmental conditions are stable during exposure. At the age of 3 weeks and 7 weeks after exposure, 8 offspring from each group were randomly stained with GFAP,NCAM and GABA receptors in hippocampus by immunohistochemical method. Then CA1, in hippocampus was analyzed by image analysis system. Protein expression of CA3 and DG region (dentate gyrus). Results there was no effect on the expression of GFAP,NCAM,GABA receptor in the hippocampus of F1 rats at the age of three weeks and seven weeks in the 1.0mW/cm2 exposure group, but there was no significant difference between the expression of GFAP,NCAM,GABA receptor and that of the control group. The expression of NCAM,GABA receptor and GFAP,NCAM,GABA receptor in hippocampus of three week old F1 generation rats in 0.5mW/cm2 exposure group were down-regulated, and the expression of GFAP,NCAM,GABA receptor was significantly lower than that of control group. Conclusion the expression of NCAM and GABA receptors in three-week-old rats and GFAP,NCAM and GABA receptors in hippocampus of 7-week-old rats were down-regulated by 1800MHz power density 0.5mW/cm2 exposure before birth.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R363

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