發(fā)布時(shí)間：2018-12-18 03:36

【摘要】： 目的了解1800MHz電磁波出生前暴露對(duì)大鼠海馬NCAM和GABA受體表達(dá)的影響 方法7周齡SD雌性大鼠32只,雄性16只,適應(yīng)環(huán)境一周后以雌：雄=2：1交配,受孕后雌鼠隨機(jī)分為4組(兩個(gè)實(shí)驗(yàn)組和兩個(gè)對(duì)照組)。實(shí)驗(yàn)組采用1800MHz射頻電磁波對(duì)大鼠于孕0～20天進(jìn)行21天連續(xù)暴露,每天12小時(shí),功率密度為0.5 mW／cm2和1.0 mW／cm2,各實(shí)驗(yàn)組分別設(shè)置虛擬暴露作為對(duì)照組。暴露期間的溫度、濕度、背景噪聲等環(huán)境條件穩(wěn)定。暴露完成后在F1代子鼠長(zhǎng)至三周齡和七周齡,每組隨機(jī)抽取8只子鼠采用免疫組織化學(xué)方法分別對(duì)海馬GFAP、NCAM及GABA受體進(jìn)行染色,然后采用圖像分析系統(tǒng)分析海馬CA1、CA3和DG區(qū)(齒狀回)的蛋白表達(dá)。 結(jié)果1.0mW／cm2暴露組未發(fā)現(xiàn)對(duì)三周齡、七周齡F1代大鼠海馬各區(qū)GFAP、NCAM、GABA受體表達(dá)有影響,其表達(dá)與對(duì)照組無統(tǒng)計(jì)學(xué)意義；0.5mW／cm2暴露組三周齡F1代大鼠海馬各區(qū)NCAM、GABA受體表達(dá)和七周齡GFAP、NCAM、GABA受體表達(dá)均出現(xiàn)了下調(diào),其表達(dá)與對(duì)照組相比有統(tǒng)計(jì)學(xué)意義。結(jié)論本實(shí)驗(yàn)條件下,1800MHz功率密度0.5mW／cm2電磁輻射出生前暴露使三周齡大鼠NCAM與GABA受體和七周齡大鼠GFAP、NCAM與GABA受體在海馬各區(qū)表達(dá)下調(diào)。
[Abstract]:Objective to investigate the effects of 1800MHz electromagnetic wave exposure before birth on the expression of NCAM and GABA receptors in hippocampus of rats. Methods Thirty-two 7-week-old SD female rats and 16 male rats were used to mate with female: male = 2:1 after adapting to the environment for one week. After conception, female rats were randomly divided into 4 groups (two experimental groups and two control groups). Rats in the experimental group were exposed to 1800MHz radio frequency electromagnetic wave for 21 days, 12 hours a day, and the power density was 0.5 mW/cm2 and 1.0 mW/cm2, respectively. Temperature, humidity, background noise and other environmental conditions are stable during exposure. At the age of 3 weeks and 7 weeks after exposure, 8 offspring from each group were randomly stained with GFAP,NCAM and GABA receptors in hippocampus by immunohistochemical method. Then CA1, in hippocampus was analyzed by image analysis system. Protein expression of CA3 and DG region (dentate gyrus). Results there was no effect on the expression of GFAP,NCAM,GABA receptor in the hippocampus of F1 rats at the age of three weeks and seven weeks in the 1.0mW/cm2 exposure group, but there was no significant difference between the expression of GFAP,NCAM,GABA receptor and that of the control group. The expression of NCAM,GABA receptor and GFAP,NCAM,GABA receptor in hippocampus of three week old F1 generation rats in 0.5mW/cm2 exposure group were down-regulated, and the expression of GFAP,NCAM,GABA receptor was significantly lower than that of control group. Conclusion the expression of NCAM and GABA receptors in three-week-old rats and GFAP,NCAM and GABA receptors in hippocampus of 7-week-old rats were down-regulated by 1800MHz power density 0.5mW/cm2 exposure before birth.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 許勤,黃建中,丁毓風(fēng),李農(nóng);手提式移動(dòng)電話微波輻射強(qiáng)度結(jié)果分析[J];安徽預(yù)防醫(yī)學(xué)雜志;1999年04期

2 張雪峰,龔志軍,侯小娟,慕利娟,陳華,崔麗珍;電磁污染的危害和防護(hù)[J];包頭鋼鐵學(xué)院學(xué)報(bào);2003年02期

3 孫艷,騰云飛,楊新華;電磁輻射的研究及其仿真平臺(tái)的建立——移動(dòng)通信基站的電磁輻射[J];大眾科技;2005年09期

4 吳錫南,程天民,劉蘋,王曉芳,方源揚(yáng),曾遠(yuǎn)全,蘇麗瓊;出生前暴露1 800MHz電磁場(chǎng)大鼠Morris迷宮測(cè)試[J];環(huán)境與健康雜志;2003年01期

5 武慧欣;劉蘋;黃春桃;靳大力;吳錫南;;1800MHz電磁波輻射對(duì)大鼠海馬細(xì)胞DNA的損傷作用[J];環(huán)境與健康雜志;2006年05期

6 金劍;李柱一;林宏;;大鼠生后中樞神經(jīng)系統(tǒng)S100B和膠質(zhì)纖維酸性蛋白表達(dá)的變化[J];解剖學(xué)報(bào);2007年03期

7 王曉亞;王增賢;王懷經(jīng);李振中;;大鼠海馬γ-氨基丁酸-A受體在生后發(fā)育中的表達(dá)[J];解剖學(xué)雜志;2006年02期

8 王葆芳,蘆秀香,徐曉波,張霖;900MHz脈沖微波近場(chǎng)輻射對(duì)實(shí)驗(yàn)大鼠學(xué)習(xí)記憶功能的影響[J];環(huán)境與職業(yè)醫(yī)學(xué);2003年04期

9 張亮,呂明;移動(dòng)通信電磁輻射初探[J];企業(yè)技術(shù)開發(fā);2004年08期

10 王楸;錢燕;陸寧潔;池美珠;李劍敏;萬麗;;宮內(nèi)手機(jī)微波輻射對(duì)新生大鼠神經(jīng)細(xì)胞粘附分子的影響[J];中國(guó)輻射衛(wèi)生;2009年01期

，

本文編號(hào)：2385268