【摘要】：目的：研究急性心理應(yīng)激對胃腸粘膜形態(tài)和屏障功能以及結(jié)腸運(yùn)動(dòng)的影響。 方法： 55只雄性wistar大鼠經(jīng)過3小時(shí)限動(dòng)束縛后隨機(jī)分入4個(gè)組：A組(3小時(shí)即時(shí)處死組)14只,B組(束縛后休息3小時(shí)處死組)14只,C組(束縛后休息9小時(shí)處死組)14只,D組(束縛后休息21小時(shí)處死組)13只；另外13只大鼠禁食禁水3小時(shí)作為對照；造模其間計(jì)數(shù)大鼠排便數(shù)。在各自時(shí)間點(diǎn)解剖大鼠采集標(biāo)本。肉眼評估胃粘膜、回腸粘膜、結(jié)腸粘膜形態(tài)；光鏡下對胃、回腸、結(jié)腸粘膜病理形態(tài)進(jìn)行觀察；免疫組化法分析回腸、結(jié)腸粘膜緊密連接Occludin蛋白的表達(dá)；TUNEL法檢測回腸上皮細(xì)胞凋亡；檢測血漿二胺氧化酶(DAO)活性和D-乳酸(D-lac)濃度。 結(jié)果： 束縛成功造成大鼠急性心理應(yīng)激；急性心理應(yīng)激導(dǎo)致大鼠胃粘膜廣泛糜爛和散在出血；急性心理應(yīng)激組緊密連接蛋白Occludin蛋白染色變淡,平均光密度略有降低,但與對照組差異不顯著；急性心理應(yīng)激組回腸粘膜上皮細(xì)胞凋亡指數(shù)明顯增高。和對照組比較,急性心理應(yīng)激各組血漿DAO活性和D-lac濃度未見明顯增高；急性心理應(yīng)激導(dǎo)致大鼠結(jié)腸運(yùn)動(dòng)增強(qiáng)。 結(jié)論： 1本束縛方法制造心理應(yīng)激模型是成功的。 2本心理應(yīng)激模型可導(dǎo)致大鼠胃腸粘膜從形態(tài)學(xué)損害到屏障功能的損害。 3本研究中大鼠腸粘膜屏障功能損害可能以細(xì)胞凋亡增加為主要途徑,細(xì)胞間隙緊密連接破壞為次要途徑。 4急性心理應(yīng)激中大鼠結(jié)腸運(yùn)動(dòng)增強(qiáng)。
[Abstract]:Aim: to study the effects of acute psychological stress on gastrointestinal mucosal morphology, barrier function and colon motility. Methods: 55 male wistar rats were randomly divided into 4 groups after 3 hours of restraint: group A (group A: n = 14), group B (group B: 3 hours after 3 hours rest), group B (n = 14). There were 14 rats in group C and 13 rats in group D (death group with 21 hours rest after restraint). The other 13 rats were fasting for 3 hours as control, and the defecation number of rats was counted. The rats were dissected at different time points. The morphology of gastric, ileal and colonic mucosa was evaluated by naked eye, the pathological morphology of gastric, ileal and colonic mucosa was observed under light microscope, the expression of Occludin protein in ileum and colonic mucosa was analyzed by immunohistochemical method. TUNEL assay was used to detect the apoptosis of ileal epithelial cells, the plasma diamine oxidase (DAO) activity and the concentration of D-lactic acid (D-lac) were detected. Results: acute psychological stress resulted in extensive erosion and scattered bleeding of gastric mucosa in rats. In acute psychological stress group, the staining of tight junction protein Occludin protein became lighter, the average optical density decreased slightly, but there was no significant difference between the acute psychological stress group and the control group, and the apoptosis index of ileal mucosal epithelial cells in the acute psychological stress group was significantly higher than that in the control group. Compared with the control group, the plasma DAO activity and D-lac concentration in the acute psychological stress groups were not significantly increased, but the colonic motility was increased in the rats under acute psychological stress. Conclusion: 1 the model of psychological stress made by this restraint method is successful. 2 the psychological stress model can cause the damage of gastrointestinal mucosa from morphological damage to barrier function in rats. 3 in this study, the main pathway of intestinal mucosal barrier dysfunction in rats was the increase of apoptosis, and the destruction of tight junction between the cells was the secondary pathway. 4 the colonic motility of rats was enhanced during acute psychological stress.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2009
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 郝悅,黃裕新,王景杰;心理性應(yīng)激對大鼠胃排空的影響及胃粘膜的損傷[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2002年07期

2 石慧琳,程義勇,李樹田,王冬蘭,于志杰,耿戰(zhàn)輝,陳偉強(qiáng),馮憑;慢性束縛應(yīng)激對大鼠腸粘膜屏障功能的影響[J];中國行為醫(yī)學(xué)科學(xué);2003年03期

3 曹碧茵,施建蓉,童瑤,鄒軍;心理應(yīng)激動(dòng)物模型的研制及其應(yīng)用[J];中國行為醫(yī)學(xué)科學(xué);2005年02期

4 桂先勇,潘國宗,柯美云,陳國鈞;胃腸肽在應(yīng)激所致結(jié)腸動(dòng)力紊亂中的作用[J];中華醫(yī)學(xué)雜志;1997年01期

5 姚詠明,于燕,盛志勇,施志國,周寶桐,田惠民;創(chuàng)傷后內(nèi)毒素血癥與臟器損害關(guān)系及其防治新方法的系列研究[J];中國危重病急救醫(yī)學(xué);1998年03期

，

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