人牙根尖乳頭細胞體外無支架三維培養(yǎng)的生物學行為研究
發(fā)布時間:2018-10-14 14:02
【摘要】:目的探討根尖乳頭干細胞(stem cells from the apical papilla,SCAP)在無支架三維培養(yǎng)條件下的生物學行為。方法用顯微鏡和HR染色觀察SCAP球的大體形態(tài),組織學結構;用免疫組化法檢測SCAP球中骨涎蛋白(bone sialoprotein,BSP)和血管性血友病因子(von Willebrand factor,vWF)的表達。結果細胞球的大小對球體內(nèi)部細胞的生長有影響,SCAP球具有向成骨和向內(nèi)皮分化的潛能。結論無支架三維培養(yǎng)是一種可行的SCAP培養(yǎng)方法。
[Abstract]:Objective to investigate the biological behavior of apical papilla stem cells (stem cells from the apical papilla,SCAP) in three-dimensional culture without scaffold. Methods the gross morphology and histological structure of SCAP balls were observed by microscope and HR staining, and the expression of bone sialoprotein,BSP and (von Willebrand factor,vWF in SCAP spheres were detected by immunohistochemistry. Results the size of the spheres had an effect on the growth of the cells inside the spheres, and the SCAP spheres had the potential of osteogenesis and endothelial differentiation. Conclusion three-dimensional culture without scaffold is a feasible method for SCAP culture.
【作者單位】: 福建醫(yī)科大學附屬口腔醫(yī)院;福建省福州市中醫(yī)院;
【基金】:福建省自然科學基金資助項目(2014J01319)
【分類號】:R329.28
本文編號:2270692
[Abstract]:Objective to investigate the biological behavior of apical papilla stem cells (stem cells from the apical papilla,SCAP) in three-dimensional culture without scaffold. Methods the gross morphology and histological structure of SCAP balls were observed by microscope and HR staining, and the expression of bone sialoprotein,BSP and (von Willebrand factor,vWF in SCAP spheres were detected by immunohistochemistry. Results the size of the spheres had an effect on the growth of the cells inside the spheres, and the SCAP spheres had the potential of osteogenesis and endothelial differentiation. Conclusion three-dimensional culture without scaffold is a feasible method for SCAP culture.
【作者單位】: 福建醫(yī)科大學附屬口腔醫(yī)院;福建省福州市中醫(yī)院;
【基金】:福建省自然科學基金資助項目(2014J01319)
【分類號】:R329.28
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