【摘要】： 目的:觀察Fe_3O_4磁流體(magnetic fluid,MF)注射到豬胰腺后的安全性,并探索采用動脈介入的方法向豬胰腺注射Fe_3O_4磁流體的可行性。 方法:1.Fe_3O_4磁流體經(jīng)手術(shù)直接局部注射到豬的胰腺,注射前后測量并對比實驗動物血淀粉酶、空腹血糖、血常規(guī)值的變化;磁流體注射6天后觀察胰腺及其他內(nèi)臟器官磁流體的分布及病理變化。2.對豬胰腺進(jìn)行解剖學(xué)觀察并繪制豬胰腺血管圖,采用seldinger法經(jīng)股動將sp管插到脾動脈,向胰腺供血動脈注射濃度為100mg/ml的Fe_3O_4磁流體1ml。磁流體注射6天后處死實驗動物,取胰腺及其他內(nèi)臟器官行組織切片HE染色。 結(jié)果:1.磁流體注射前后實驗動物血淀粉酶、空腹血糖、血常規(guī)值均無明顯變化,磁流體沉積于注射部位,磁流體周圍胰腺組織有巨噬細(xì)胞浸潤,其他臟器沒有明顯磁流體分布,組織結(jié)構(gòu)正常。2.實驗成功繪制了胰腺血管供應(yīng)圖,通過動脈介入的方式成功向胰腺內(nèi)注射磁流體,經(jīng)病檢顯示磁流體較好地分布于注射部位胰腺內(nèi),其他臟器未見明顯的磁流體分布。 結(jié)論:通過對實驗動物血淀粉酶、空腹血糖、血常規(guī)等指標(biāo)的檢測可以觀察到Fe_3O_4磁流體胰腺注射是安全可行的。我們成功繪制了胰腺動脈血管圖,將為人類胰腺疾病的豬模型實驗研究提供參考。實驗結(jié)果顯示采用動脈介入的方式向胰腺內(nèi)注射磁流體是可行的,且沒有引起胰腺組織的明顯損傷,這一技術(shù)可用于進(jìn)一步的胰腺癌磁靶向熱療研究。
[Abstract]:Aim: to observe the safety of Fe_3O_4 magnetic fluid (magnetic fluid,MF) injection into porcine pancreas and to explore the feasibility of injecting Fe_3O_4 magnetic fluid into porcine pancreas by arterial intervention. Methods 1. Fe3O4 magnetic fluid was injected directly into pig pancreas after operation. The changes of serum amylase, fasting blood glucose and blood routine were measured and compared before and after injection. The distribution and pathological changes of magnetic fluid in pancreas and other visceral organs were observed 6 days after MHD injection. The porcine pancreas was anatomically observed and the porcine pancreatic angiogram was drawn. The sp tube was inserted into the splenic artery by seldinger method and the Fe_3O_4 magnetic fluid containing 100mg/ml was injected into the pancreatic artery. The animals were killed 6 days after magnetic fluid injection. The pancreas and other visceral organs were taken for HE staining. The result is 1: 1. Before and after magnetic fluid injection, there were no significant changes in serum amylase, fasting blood glucose, blood routine value, magnetic fluid deposition in the injection site, infiltration of macrophages in pancreatic tissue around magnetic fluid, and no obvious distribution of magnetic fluid in other organs. Normal structure. The pancreatic vascular supply map was successfully drawn, and the magnetic fluid was injected into the pancreas successfully by arterial intervention. The pathological examination showed that the magnetic fluid was well distributed in the pancreas at the injection site, but there was no obvious magnetic fluid distribution in other organs. Conclusion: it is safe and feasible to observe Fe_3O_4 magnetic fluid pancreatic injection by detecting serum amylase, fasting blood glucose and blood routine. We successfully mapped the pancreatic artery, which will provide reference for the pig model study of human pancreatic disease. The experimental results show that it is feasible to inject magnetic fluid into the pancreas by arterial intervention without causing obvious damage to pancreatic tissue. This technique can be used in the further study of magnetic targeted hyperthermia of pancreatic cancer.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R735.9;R312

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