多糖類提取物及MF59佐劑對流感滅活疫苗免疫增強作用研究
發(fā)布時間：2018-09-04 13:12
【摘要】：本論文由兩部分組成：中藥多糖類提取物對流感滅活疫苗的免疫增強作用研究；MF59佐劑于不同免疫途徑下作流感滅活疫苗的佐劑效果比較研究。 (一)中藥多糖類提取物對流感滅活疫苗的免疫增強作用研究 (1)枸杞多糖(LBP)、黃芪多糖(APS)對A／PR／8流感滅活疫苗的體液免疫應(yīng)答作用研究。不同劑量的LBP (300μg、500μg和800μg)或APS(300μg和500μg),及100μg的氫氧化鋁分別與A／PR／8疫苗混合,經(jīng)i.p.或i.m.兩條免疫途徑共免疫小鼠兩次,間隔三周。ELISA檢測實驗結(jié)果表明,LBP、APS無論經(jīng)i.p.或i.m.免疫,均能明顯提高小鼠的抗體滴度,且呈現(xiàn)一定的劑量依賴性；高劑量的LBP及APS佐劑效果與氫氧化鋁相當(dāng)。 (2)茯苓多糖(pachymaran)能否作為低劑量(0.015μg)或高劑量(1.5μg)A／PR／8流感滅活疫苗的佐劑,增強疫苗的免疫原性。不同劑量的pachymaran(200μg和100μg)或100μg的氫氧化鋁與A／PR／8流感滅活疫苗經(jīng)i.m.共免疫小鼠,一免后三周通過檢測抗體水平、病毒攻擊后小鼠的體重變化及保護率等幾個方面檢測其效果。結(jié)果如下： ①pachymaran對低劑量A／PR／8疫苗的佐劑效應(yīng) 1000μg pachymaran IgG、IgG1、IgG2a抗體滴度均顯著高于200μg pachymaran組和單獨A／PR／8疫苗組；攻毒后明顯降低了小鼠的體重丟失及肺部病毒量,提高小鼠的存活率,保護效果與氫氧化鋁相當(dāng)。 ②pachymaran對高劑量A／PR／8疫苗的佐劑效應(yīng) Pachymaran仍能提高小鼠的IgG.IgG1、IgG2a抗體滴度,效果卻遜于鋁佐劑組,然而病毒攻擊后,其體重丟失率卻與鋁佐劑組差異不大。 (二)檢測MF59于不同免疫途徑下的佐劑效果。將MF59與A／PR／8疫苗混合,分別經(jīng)i.p.、i.m.、i.v.和i.n.共免疫小鼠一次或兩次,間隔三周,一免后四周或加強免疫后一周用致死量流感病毒攻擊小鼠,檢測小鼠血清抗體滴度、體重丟失率及保護率。結(jié)果表明i.p.優(yōu)于i.m.;i.v.產(chǎn)生的抗體效價與i.m.相當(dāng),然而保護效果卻遜于i.m.；i.n.最差。
[Abstract]:This paper consists of two parts: the study on the immune enhancement effect of Chinese medicine polysaccharides extract on inactivated influenza vaccine; the comparative study on the adjuvant effect of MF59 adjuvant on inactivated influenza vaccine under different immune routes. (1) study on the immune Enhancement of Polysaccharide extracts from Chinese Medicine on Influenza inactivated Vaccine (1) Humoral immune response of Lycium barbarum Polysaccharide (LBP), Astragalus Polysaccharide (APS) to A/PR/8 Influenza inactivated Vaccine. Different doses of LBP (300 渭 g, 500 渭 g and 800 渭 g) or APS (300 渭 g, 500 渭 g), and 100 渭 g aluminum hydroxide) were mixed with A/PR/8 vaccine, respectively. Or i.m. Mice were immunized twice with three weeks interval. The results of Elisa showed that LBP- APS could be immunized by i.p. Or i.m. The antibody titers of mice were significantly increased by immunization, and the antibody titers were in a dose-dependent manner. (2) whether Poria cocos polysaccharide (pachymaran) can be used as adjuvant of low dose (0.015 渭 g) or high dose (1.5 渭 g) A/PR/8 inactivated vaccine to enhance the immunogenicity of the vaccine. Different doses of pachymaran (200 渭 g and 100 渭 g) or 100 渭 g aluminum hydroxide and A/PR/8 inactivated vaccine were inoculated with i.m. Three weeks after immunization, the antibody level, the weight change and the protective rate of the mice after virus attack were detected. The results were as follows: the adjuvant effect of 1pachymaran on low dose A/PR/8 vaccine was significantly higher than that of 1000 渭 g pachymaran IgG,IgG1,IgG2a antibody in low dose A/PR/8 vaccine group and only A/PR/8 vaccine group. After attacking the virus, the weight loss and lung virus content of the mice were significantly reduced, and the survival rate of the mice was increased. The protective effect was similar to that of aluminum hydroxide. The adjuvant effect of 2pachymaran on high dose A/PR/8 vaccine Pachymaran could still increase the titer of IgG.IgG1,IgG2a antibody in mice, but the effect was inferior to that of aluminum adjuvant group, but after virus attack, The weight loss rate was not significantly different from that in the aluminum adjuvant group. (2) to detect the adjuvant effect of MF59 in different immune pathways. The mixture of MF59 and A/PR/8 vaccine was separated by I. P. And i.n. Mice were immunized once or twice for three weeks, four weeks after immunization or one week after booster immunization with lethal influenza virus. The titer of serum antibody, the rate of weight loss and the rate of protection were detected. The results showed that i.p. It is superior to I. The titer of antibody produced was higher than that of i.m. However, the protective effect was inferior to that of I. The worst.
【學(xué)位授予單位】：湖南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R392
本文編號：2222204
[Abstract]:This paper consists of two parts: the study on the immune enhancement effect of Chinese medicine polysaccharides extract on inactivated influenza vaccine; the comparative study on the adjuvant effect of MF59 adjuvant on inactivated influenza vaccine under different immune routes. (1) study on the immune Enhancement of Polysaccharide extracts from Chinese Medicine on Influenza inactivated Vaccine (1) Humoral immune response of Lycium barbarum Polysaccharide (LBP), Astragalus Polysaccharide (APS) to A/PR/8 Influenza inactivated Vaccine. Different doses of LBP (300 渭 g, 500 渭 g and 800 渭 g) or APS (300 渭 g, 500 渭 g), and 100 渭 g aluminum hydroxide) were mixed with A/PR/8 vaccine, respectively. Or i.m. Mice were immunized twice with three weeks interval. The results of Elisa showed that LBP- APS could be immunized by i.p. Or i.m. The antibody titers of mice were significantly increased by immunization, and the antibody titers were in a dose-dependent manner. (2) whether Poria cocos polysaccharide (pachymaran) can be used as adjuvant of low dose (0.015 渭 g) or high dose (1.5 渭 g) A/PR/8 inactivated vaccine to enhance the immunogenicity of the vaccine. Different doses of pachymaran (200 渭 g and 100 渭 g) or 100 渭 g aluminum hydroxide and A/PR/8 inactivated vaccine were inoculated with i.m. Three weeks after immunization, the antibody level, the weight change and the protective rate of the mice after virus attack were detected. The results were as follows: the adjuvant effect of 1pachymaran on low dose A/PR/8 vaccine was significantly higher than that of 1000 渭 g pachymaran IgG,IgG1,IgG2a antibody in low dose A/PR/8 vaccine group and only A/PR/8 vaccine group. After attacking the virus, the weight loss and lung virus content of the mice were significantly reduced, and the survival rate of the mice was increased. The protective effect was similar to that of aluminum hydroxide. The adjuvant effect of 2pachymaran on high dose A/PR/8 vaccine Pachymaran could still increase the titer of IgG.IgG1,IgG2a antibody in mice, but the effect was inferior to that of aluminum adjuvant group, but after virus attack, The weight loss rate was not significantly different from that in the aluminum adjuvant group. (2) to detect the adjuvant effect of MF59 in different immune pathways. The mixture of MF59 and A/PR/8 vaccine was separated by I. P. And i.n. Mice were immunized once or twice for three weeks, four weeks after immunization or one week after booster immunization with lethal influenza virus. The titer of serum antibody, the rate of weight loss and the rate of protection were detected. The results showed that i.p. It is superior to I. The titer of antibody produced was higher than that of i.m. However, the protective effect was inferior to that of I. The worst.
【學(xué)位授予單位】：湖南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2008
【分類號】：R392
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