人外泌汗腺上皮細胞原代培養(yǎng)的改良
發(fā)布時間：2018-09-01 10:09
【摘要】： 目的對人外泌汗腺上皮細胞原代培養(yǎng)的傳統(tǒng)技術(shù)進行改良,簡化實驗操作,減輕勞動強度,以期用更短的時間獲得更多量穩(wěn)定生長的外泌汗腺上皮細胞,為體外研究汗腺和構(gòu)建功能性組織工程皮膚提供理論依據(jù)和技術(shù)支持。 方法在傳統(tǒng)的汗腺組織塊培養(yǎng)法基礎(chǔ)上,于分離的人外泌汗腺組織塊表面加蓋玻片以固定組織塊,以促進汗腺組織塊貼壁,減少換液等操作所致的組織塊損失,并簡化實驗步驟。 結(jié)果改良的實驗方法能顯著提高汗腺組織塊的貼壁率,且對汗腺上皮細胞的增殖能力無明顯影響,因而在相同時間內(nèi)獲得的汗腺上皮細胞數(shù)量比傳統(tǒng)方法更多。 結(jié)論與傳統(tǒng)的組織塊培養(yǎng)法相比,本方法的實驗步驟簡單,耗時更少,在相同單位時間內(nèi)獲得的細胞數(shù)量更多。培養(yǎng)的人外泌汗腺上皮細胞保持了上皮細胞的結(jié)構(gòu)特點,且具有良好活力,說明了人工構(gòu)建外泌汗腺的可行性。
[Abstract]:Objective to improve the traditional technique of primary culture of human exocrine sweat gland epithelial cells, simplify the experimental operation and reduce the labor intensity, so as to obtain more stable growth of exocrine sweat gland epithelial cells in a shorter time. To provide theoretical basis and technical support for the study of sweat glands and the construction of functional tissue engineering skin in vitro. Methods on the basis of the traditional culture method of sweat gland tissue block, a cover glass was added on the surface of the human exocrine sweat gland tissue block to fix the tissue mass, so as to promote the adhesion of the sweat gland tissue mass to the wall and reduce the loss of tissue mass caused by the operation of fluid exchange. The experimental steps are simplified. Results the modified method could significantly increase the adherent rate of sweat gland tissue mass, and had no obvious effect on the proliferation of sweat gland epithelial cells. Therefore, the number of sweat gland epithelial cells obtained at the same time was more than that of the traditional method. Conclusion compared with the traditional tissue mass culture method, this method has the advantages of simple experimental steps, less time consuming and more cells obtained in the same unit time. The cultured human eccrine sweat gland epithelial cells maintain the structural characteristics of epithelial cells and have good vitality, which indicates the feasibility of artificial construction of exocrine sweat glands.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R329.2
本文編號：2216873
[Abstract]:Objective to improve the traditional technique of primary culture of human exocrine sweat gland epithelial cells, simplify the experimental operation and reduce the labor intensity, so as to obtain more stable growth of exocrine sweat gland epithelial cells in a shorter time. To provide theoretical basis and technical support for the study of sweat glands and the construction of functional tissue engineering skin in vitro. Methods on the basis of the traditional culture method of sweat gland tissue block, a cover glass was added on the surface of the human exocrine sweat gland tissue block to fix the tissue mass, so as to promote the adhesion of the sweat gland tissue mass to the wall and reduce the loss of tissue mass caused by the operation of fluid exchange. The experimental steps are simplified. Results the modified method could significantly increase the adherent rate of sweat gland tissue mass, and had no obvious effect on the proliferation of sweat gland epithelial cells. Therefore, the number of sweat gland epithelial cells obtained at the same time was more than that of the traditional method. Conclusion compared with the traditional tissue mass culture method, this method has the advantages of simple experimental steps, less time consuming and more cells obtained in the same unit time. The cultured human eccrine sweat gland epithelial cells maintain the structural characteristics of epithelial cells and have good vitality, which indicates the feasibility of artificial construction of exocrine sweat glands.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R329.2
【引證文獻】
相關(guān)碩士學位論文 前1條
1 白亮;大鼠胰腺腺泡細胞TAP與HMGB1表達的相關(guān)性[D];遵義醫(yī)學院;2010年
，本文編號：2216873
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