【摘要】：[摘要]目的：研究大鼠放射性腎損傷影像學(xué)模型制作方法,研究早期放射性腎損傷病理改變,動態(tài)觀察大鼠單側(cè)放射性腎損傷CT、MRI表現(xiàn)、彩色多普勒B超影像學(xué)表現(xiàn)及研究腎臟血流動力學(xué)改變,并評估放射性腎病的嚴(yán)重程度。 方法：80只SD大鼠隨機(jī)分為對照組4組,照射組4組,每組10只,左腎單次分割20Gy電子線照射。照射后第4、12、24、36周分別行CT、MRI檢測,觀察影像學(xué)改變,照射后第4、12、24、36周用彩色多普勒超聲檢查,觀察影像學(xué)改變并檢測各時期腎血管多普勒參數(shù)的動態(tài)變化,同時觀察病理改變。 結(jié)果：CT、MRI均顯示大鼠放射性腎損傷,CT掃描顯示第4周、第12周對照組與照射組左腎體積差異無統(tǒng)計學(xué)意義(P0.05),第24周、第36周對照組與照射組左腎體積值可見差異具有統(tǒng)計學(xué)意義(P0.05),其照射組左腎體積低于對照組體積值。照射組不同時間組體積值差異具有統(tǒng)計學(xué)意義(P0.05),隨時間延長體積縮小。第4周對照組與照射組左腎腎實質(zhì)厚度值差異無統(tǒng)計學(xué)意義(P0.05),第12周、第24周及第36周對照組與照射組左腎腎實質(zhì)厚度值可見差異具有統(tǒng)計學(xué)意義(P0.05),其照射組左腎腎實質(zhì)厚度低于對照組。照射組不同時間左腎腎實質(zhì)厚度值差異具有統(tǒng)計學(xué)意義(P0.05),隨時間延長腎實質(zhì)厚度減少。MRI掃描顯示第4周、第12周及第24周對照組與照射組大鼠左腎體積差異無統(tǒng)計學(xué)意義(P0.05),第36周對照組與照射組左腎體積值差異具有統(tǒng)計學(xué)意義(P0.05),其照射組左腎體積低于對照組體積值。照射組不同時間體積值差異具有統(tǒng)計學(xué)意義(F為3.821,P值0.05),隨時間延長體積縮小。第4周、第12周對照組與照射組左腎腎實質(zhì)厚度值差異無統(tǒng)計學(xué)意義(P0.05),第24周及第36周對照組與照射組左腎腎實質(zhì)厚度值差異具有統(tǒng)計學(xué)意義(P0.05),其照射組左腎腎實質(zhì)厚度低于對照組。照射組不同時間左腎腎實質(zhì)厚度值差異具有統(tǒng)計學(xué)意義(P值0.05),隨時間延長腎實質(zhì)厚度減少。MRI可顯示皮髓質(zhì)分界不清。彩色多普勒B超顯示腎實質(zhì)回聲逐漸增強(qiáng),照射后第24周及第36周,腎臟體積及腎實質(zhì)厚度與對照組比較腎臟體積縮小,實質(zhì)變薄,差異有統(tǒng)計學(xué)意義(P0.05)。腎血管血流阻力指數(shù)(RI)逐漸升高,照射后36周,腎動脈RI值與對照組比較增高明顯,差異有統(tǒng)計學(xué)意義(P0.05),照射后第24周及第36周,腎內(nèi)動脈RI值與對照組比較明顯增高,差異有統(tǒng)計學(xué)意義(P0.05)。病理檢查顯示4周后出現(xiàn)腎小球及腎小管損傷,并隨時間逐漸加重。 結(jié)論： SD大鼠單側(cè)腎單分割20Gy可以制作放射性腎損傷模型,腎臟發(fā)生明顯病理變化。CT、MRI均可作為診斷放射性腎損傷的方法。彩色多普勒超聲可以作為放射性腎損傷的臨床檢查手段,其血流動力學(xué)改變可反映放射性腎病病變嚴(yán)重程度。
[Abstract]:Objective: to study the imaging model of radiation-induced renal injury in rats, to study the pathological changes of early radiation-induced renal injury, and to dynamically observe the CT MRI findings of unilateral radiation-induced renal injury in rats. Color Doppler ultrasonography (CDFB) was used to study renal hemodynamic changes and to evaluate the severity of radiation nephropathy. Methods 80 Sprague-Dawley rats were randomly divided into control group (n = 4) and irradiation group (n = 10). The imaging changes were observed by CT MRI at 36-week after irradiation. The imaging changes were observed by color Doppler ultrasound at 36-week after irradiation. The changes of renal Doppler parameters were observed and the pathological changes were observed at the same time. Results CT scan showed that there was no significant difference in left renal volume between the control group and the irradiation group at the 12th week (P0.05). At the 36th week, there was significant difference in left renal volume between the control group and the irradiation group (P0.05), and the left renal volume in the irradiation group was lower than that in the control group. The volume value of different time groups in irradiation group was significantly different (P0.05), and the volume decreased with time. There was no significant difference in left renal parenchyma thickness between the control group and the irradiation group at the 4th week (P0.05). The left renal parenchyma thickness in the control group and the irradiation group was significantly lower than that in the control group at the 24th and 36th week (P0.05). The thickness of left renal parenchyma in the irradiation group was significantly different at different time (P0.05). The thickness of renal parenchyma decreased with time. MRI scan showed that the thickness of renal parenchyma decreased in the 4th week. At week 12 and week 24, there was no significant difference in left renal volume between the control group and the irradiation group (P0.05), but there was significant difference in the left renal volume between the control group and the irradiation group at the 36th week (P0.05), and the left renal volume in the irradiation group was lower than that in the control group. The volume value of irradiation group was significantly different at different time (F = 3.821, P = 0.05), and the volume decreased with the prolongation of time. Week 4, There was no significant difference in left renal parenchyma thickness between the control group and the irradiation group at the 12th week (P0.05), but there was significant difference in the left renal parenchyma thickness between the control group and the irradiation group at the 24th week and the 36th week (P0.05), and the thickness of the left kidney parenchyma in the irradiation group was lower than that in the control group. There was significant difference in left renal parenchyma thickness between different time groups (P 0.05). The thickness of renal parenchyma decreased with time. MRI could show that the boundary of dermatomedullary was not clear. Color Doppler ultrasound showed that the echo of renal parenchyma was gradually enhanced. At the 24th and 36th week after irradiation, the renal volume and the thickness of renal parenchyma were smaller and thinner than those of the control group (P0.05). Renal vascular resistance index (RI) increased gradually, and the RI value of renal artery increased significantly at 36 weeks after irradiation (P0.05), and the RI value of renal artery was significantly higher than that of control group at the 24th and 36th week after irradiation. The difference was statistically significant (P0.05). Pathological examination showed that glomerular and tubular injury occurred 4 weeks later and gradually aggravated with time. Conclusion: the model of radiation-induced renal injury can be made in SD rats with unilateral renal monolithral 20Gy, and the pathological changes of the kidney can be used as a diagnostic method for radiation-induced renal injury. Color Doppler ultrasound can be used as a clinical examination method for radiation-induced renal injury, and its hemodynamic changes can reflect the severity of radiation-induced nephropathy.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R73-3;R-332

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相關(guān)期刊論文 前1條

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