【摘要】：目的探討MF59佐劑對腸道病毒71型(enterovirus 71,EV71)滅活疫苗誘導小鼠體液免疫應答的影響。方法將ICR小鼠隨機分成10組:MF59佐劑+不同含量EV71滅活抗原(50 EU、100 EU)組、Al(OH)3佐劑0.5 mg+不同含量EV71滅活抗原(50 EU、100 EU)組和PBS陰性對照組,每組10只,分別經(jīng)小鼠后肢肌肉和腹腔注射,200μl/只,共免疫2次,間隔4周(0、28 d)。分別于初免和加強免疫后28 d,采集小鼠尾靜脈血,分離血清,采用微量中和試驗法檢測小鼠血清抗體水平。結(jié)果除陰性對照組外,各組小鼠血清抗體陽轉(zhuǎn)率及中和抗體GMT值均隨免疫劑量和免疫時間的延長呈升高趨勢。初免后28 d,抗原含量為50 EU(低劑量)和100 EU(高劑量)各組中和抗體GMT均較低,組間差異均無統(tǒng)計學意義(P0.05)。加強免疫后28 d,低劑量各組小鼠血清中和抗體GMT與初免比較均略有升高(P0.05);高劑量各組小鼠血清中和抗體GMT與初免比較均明顯升高,其中MF59佐劑肌肉注射組血清中和抗體GMT值最高(274.40),顯著高于鋁佐劑肌肉注射組(P0.05)。結(jié)論 MF59佐劑可顯著增強EV71滅活疫苗誘導小鼠的體液免疫應答。
[Abstract]:Objective to investigate the effect of MF59 adjuvant on humoral immune response induced by inactivated vaccine of enterovirus 71 (enterovirus 71) in mice. Methods ICR mice were randomly divided into 10 groups (10 mice in each group) treated with different contents of EV71 inactivated antigen (50 EU100EU) and 50 (OH) 3 adjuvant 0.5 mg EV71 inactivated antigen (50 EUL 100EU) and PBS negative control group (10 mice). Immunization 2 times, at intervals of 4 weeks (0: 28 d).) Blood samples were collected from caudal vein of mice at 28 days after immunization, and the serum levels of antibody were detected by microneutralization test. Results except the negative control group, the serum antibody positive conversion rate and neutralizing antibody GMT value of each group showed an increasing trend with the prolongation of the immune dose and immune time. At 28 days after immunization, the GMT levels of neutralizing antibodies in 50 EU (low dose) and 100EU (high dose) groups were lower, and there was no significant difference between the two groups (P0.05). On the 28th day after immunization, the serum neutralization antibody (GMT) of low dose groups was slightly higher than that of the initial immunity (P0.05), and the serum neutralizing antibody GMT of the high dose groups was significantly higher than that of the initial immunity. The serum neutralization antibody GMT value of MF59 adjuvant injection group was the highest (274.40), which was significantly higher than that of aluminum adjuvant intramuscular injection group (P0.05). Conclusion MF59 adjuvant can significantly enhance the humoral immune response induced by EV71 inactivated vaccine in mice.
【作者單位】： 中國醫(yī)學科學院北京協(xié)和醫(yī)學院醫(yī)學生物學研究所病毒疫苗研究組云南省重大傳染病疫苗研發(fā)重點實驗室;諾華(中國)生物醫(yī)學研究有限公司;
【分類號】：R392

【參考文獻】

相關(guān)期刊論文 前1條

1 魯衛(wèi)衛(wèi);吳蘊怡;郝春生;宋冬梅;馬淑花;劉宇;陳蕾;李秀玲;;腸道病毒71型滅活疫苗的小鼠細胞免疫效果[J];中國生物制品學雜志;2014年01期

【共引文獻】

相關(guān)期刊論文 前2條

1 張雪梅;徐婧雯;吳忠香;王葳;徐小寧;董少忠;;MF59佐劑對脊髓灰質(zhì)炎滅活疫苗誘導恒河猴細胞免疫應答的影響[J];中國生物制品學雜志;2014年10期

2 陸鳳;譚磊;王欣;包世俊;任峰;張凡慶;劉芳;仇旭升;宋翠萍;孫英杰;伏小平;丁鏟;;滑液支原體滅活疫苗體外誘導T淋巴細胞增殖及免疫保護研究[J];浙江農(nóng)業(yè)學報;2015年06期

【相似文獻】

相關(guān)期刊論文 前2條

1 李群英;用簡單的純亞微米級油/水乳劑佐劑MF59可增強對人流感疫苗的體液應答[J];國外醫(yī)學.預防.診斷.治療用生物制品分冊;1996年06期

2 ;[J];;年期

相關(guān)碩士學位論文 前1條

1 謝國秀;多糖類提取物及MF59佐劑對流感滅活疫苗免疫增強作用研究[D];湖南師范大學;2008年

，

本文編號：2167858