發(fā)布時(shí)間：2018-08-06 13:22

【摘要】：目的探討MF59佐劑對(duì)腸道病毒71型(enterovirus 71,EV71)滅活疫苗誘導(dǎo)小鼠體液免疫應(yīng)答的影響。方法將ICR小鼠隨機(jī)分成10組:MF59佐劑+不同含量EV71滅活抗原(50 EU、100 EU)組、Al(OH)3佐劑0.5 mg+不同含量EV71滅活抗原(50 EU、100 EU)組和PBS陰性對(duì)照組,每組10只,分別經(jīng)小鼠后肢肌肉和腹腔注射,200μl/只,共免疫2次,間隔4周(0、28 d)。分別于初免和加強(qiáng)免疫后28 d,采集小鼠尾靜脈血,分離血清,采用微量中和試驗(yàn)法檢測(cè)小鼠血清抗體水平。結(jié)果除陰性對(duì)照組外,各組小鼠血清抗體陽轉(zhuǎn)率及中和抗體GMT值均隨免疫劑量和免疫時(shí)間的延長(zhǎng)呈升高趨勢(shì)。初免后28 d,抗原含量為50 EU(低劑量)和100 EU(高劑量)各組中和抗體GMT均較低,組間差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。加強(qiáng)免疫后28 d,低劑量各組小鼠血清中和抗體GMT與初免比較均略有升高(P0.05);高劑量各組小鼠血清中和抗體GMT與初免比較均明顯升高,其中MF59佐劑肌肉注射組血清中和抗體GMT值最高(274.40),顯著高于鋁佐劑肌肉注射組(P0.05)。結(jié)論 MF59佐劑可顯著增強(qiáng)EV71滅活疫苗誘導(dǎo)小鼠的體液免疫應(yīng)答。
[Abstract]:Objective to investigate the effect of MF59 adjuvant on humoral immune response induced by inactivated vaccine of enterovirus 71 (enterovirus 71) in mice. Methods ICR mice were randomly divided into 10 groups (10 mice in each group) treated with different contents of EV71 inactivated antigen (50 EU100EU) and 50 (OH) 3 adjuvant 0.5 mg EV71 inactivated antigen (50 EUL 100EU) and PBS negative control group (10 mice). Immunization 2 times, at intervals of 4 weeks (0: 28 d).) Blood samples were collected from caudal vein of mice at 28 days after immunization, and the serum levels of antibody were detected by microneutralization test. Results except the negative control group, the serum antibody positive conversion rate and neutralizing antibody GMT value of each group showed an increasing trend with the prolongation of the immune dose and immune time. At 28 days after immunization, the GMT levels of neutralizing antibodies in 50 EU (low dose) and 100EU (high dose) groups were lower, and there was no significant difference between the two groups (P0.05). On the 28th day after immunization, the serum neutralization antibody (GMT) of low dose groups was slightly higher than that of the initial immunity (P0.05), and the serum neutralizing antibody GMT of the high dose groups was significantly higher than that of the initial immunity. The serum neutralization antibody GMT value of MF59 adjuvant injection group was the highest (274.40), which was significantly higher than that of aluminum adjuvant intramuscular injection group (P0.05). Conclusion MF59 adjuvant can significantly enhance the humoral immune response induced by EV71 inactivated vaccine in mice.
【作者單位】： 中國(guó)醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院醫(yī)學(xué)生物學(xué)研究所病毒疫苗研究組云南省重大傳染病疫苗研發(fā)重點(diǎn)實(shí)驗(yàn)室;諾華(中國(guó))生物醫(yī)學(xué)研究有限公司;
【分類號(hào)】：R392

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