北京地區(qū)漢族絕經(jīng)后婦女MATN3基因多態(tài)性與骨質(zhì)疏松表型的關(guān)聯(lián)研究
發(fā)布時間：2018-07-23 16:36
【摘要】：目的：MATN3基因編碼胞外基質(zhì)蛋白,并可能對軟骨分化過程起調(diào)控作用。本研究的目的是探討MATN3基因多態(tài)性與絕經(jīng)后婦女骨密度、骨折、椎體骨折、骨轉(zhuǎn)換標志物和25(OH)VitD的關(guān)系。 方法：在北京地區(qū)隨機抽樣收集1488名漢族絕經(jīng)后婦女作為研究對象,通過問卷調(diào)查及胸腰椎X線片閱讀分別確定骨折及椎體骨折表型。以雙能X線吸收儀檢測腰椎(L2-4)、股骨頸和全髖的骨密度,以羅氏自動檢測儀采用電化學發(fā)光免疫測定法檢測血清β-CTX、P1NP和25(OH)VitD水平。使用TaqMan基因分型技術(shù)檢測MATN3基因4個標簽SNP的多態(tài)性。采用logistic回歸等統(tǒng)計學方法分析SNP位點基因型和單體型與各骨質(zhì)疏松表型的關(guān)系,以相關(guān)分析等方法分析骨轉(zhuǎn)換標志物和25(OH)VitD與年齡、骨密度、骨折和椎體骨折的關(guān)系。 結(jié)果： 1. MATN3基因rs11096633位點和rs6734005位點的多態(tài)性與全髖骨密度顯著相關(guān)(P值分別為0.000-0.032和0.005-0.026),并且這種顯著性經(jīng)過對多重檢驗的校正后仍然存在。其中rs6734005位點的少見等位基因A對髖部骨密度起保護作用。rs10178256位點與全髖骨密度的相關(guān)性也具有提示意義(p=0.009-0.037)。單體型6AC與全髖骨密度顯著相關(guān)(p=0.005-0.023),對髖部骨密度起保護作用,其顯著性經(jīng)過對多重檢驗校正后仍有統(tǒng)計學意義。 2. MATN3基因多態(tài)性與β-CTX、P1NP和25(OH)VitD水平無關(guān)。 3.絕經(jīng)后婦女β-CTX和P1NP水平隨年齡增長呈現(xiàn)先下降后上升的趨勢,并與腰椎、股骨頸和全髖的骨密度呈顯著負相關(guān)。北京地區(qū)絕經(jīng)后婦女的血清25(OH)VitD水平明顯偏低(13.10±5.37ng/ml),25(OH)VitD水平隨著年齡的增長而逐漸下降。本研究未發(fā)現(xiàn)β-CTX、P1NP和25(OH)VitD與骨折、椎體骨折存在相關(guān)性。 結(jié)論：本研究首次揭示MATN3基因多態(tài)性可能影響絕經(jīng)后婦女全髖骨密度的變異,而與骨轉(zhuǎn)換標志物和維生素D水平?jīng)]有相關(guān)性。
[Abstract]:Objective to encode extracellular matrix protein (ECM) and regulate cartilage differentiation. The aim of this study was to investigate the association of MATN3 gene polymorphism with bone mineral density, fracture, vertebral fracture, bone turnover marker and 25 (OH) VitD in postmenopausal women. Methods: 1488 postmenopausal women of Han nationality were randomly sampled in Beijing area. The phenotypes of fracture and vertebral body fracture were determined by questionnaire survey and X-ray reading of thoracolumbar vertebrae. Bone mineral density (BMD) of lumbar spine (L2-4), femoral neck and total hip were measured by dual energy X-ray absorptiometry. Serum 尾 -CTXT P1NP and 25 (OH) VitD levels were measured by electrochemiluminescence immunoassay with Roche automatic detector. TaqMan genotyping technique was used to detect the polymorphism of SNP tagged MATN3 gene. The relationship between the genotype and haplotype of SNP locus and the phenotypes of osteoporosis was analyzed by logistic regression, and the relationship between bone turnover markers and 25 (OH) VitD with age, bone density, fracture and vertebral fracture was analyzed by correlation analysis. Results: 1. The polymorphism of rs11096633 locus and rs6734005 locus of MATN3 gene was significantly associated with total hip bone density (P = 0.000-0.032 and 0.005-0.026, respectively). The rare allele A of rs6734005 locus has protective effect on hip bone density. The correlation between rs10178256 locus and total hip bone density is also significant (p 0.009-0.037). Haplotype 6AC was significantly correlated with total hip bone density (p0. 005-0. 023) and had a protective effect on hip bone density. The polymorphism of MATN3 gene was not associated with the levels of 尾 -CTXG P1NP and 25 (OH) VitD. The levels of 尾 -CTX and P1NP in postmenopausal women decreased first and then increased with age, and were negatively correlated with bone mineral density of lumbar vertebrae, femoral neck and total hip. The serum 25 (OH) VitD level of postmenopausal women in Beijing was significantly lower than that of postmenopausal women (13.10 鹵5.37ng/ml). There was no correlation between 尾-CTX P1NP and 25 (OH) VitD and vertebral fracture. Conclusion: MATN3 gene polymorphism may affect the variation of total hip bone density in postmenopausal women for the first time, but has no correlation with bone turnover markers and vitamin D levels.
【學位授予單位】：北京協(xié)和醫(yī)學院
【學位級別】：博士
【學位授予年份】：2010
【分類號】：R580;R3416
本文編號：2139996
[Abstract]:Objective to encode extracellular matrix protein (ECM) and regulate cartilage differentiation. The aim of this study was to investigate the association of MATN3 gene polymorphism with bone mineral density, fracture, vertebral fracture, bone turnover marker and 25 (OH) VitD in postmenopausal women. Methods: 1488 postmenopausal women of Han nationality were randomly sampled in Beijing area. The phenotypes of fracture and vertebral body fracture were determined by questionnaire survey and X-ray reading of thoracolumbar vertebrae. Bone mineral density (BMD) of lumbar spine (L2-4), femoral neck and total hip were measured by dual energy X-ray absorptiometry. Serum 尾 -CTXT P1NP and 25 (OH) VitD levels were measured by electrochemiluminescence immunoassay with Roche automatic detector. TaqMan genotyping technique was used to detect the polymorphism of SNP tagged MATN3 gene. The relationship between the genotype and haplotype of SNP locus and the phenotypes of osteoporosis was analyzed by logistic regression, and the relationship between bone turnover markers and 25 (OH) VitD with age, bone density, fracture and vertebral fracture was analyzed by correlation analysis. Results: 1. The polymorphism of rs11096633 locus and rs6734005 locus of MATN3 gene was significantly associated with total hip bone density (P = 0.000-0.032 and 0.005-0.026, respectively). The rare allele A of rs6734005 locus has protective effect on hip bone density. The correlation between rs10178256 locus and total hip bone density is also significant (p 0.009-0.037). Haplotype 6AC was significantly correlated with total hip bone density (p0. 005-0. 023) and had a protective effect on hip bone density. The polymorphism of MATN3 gene was not associated with the levels of 尾 -CTXG P1NP and 25 (OH) VitD. The levels of 尾 -CTX and P1NP in postmenopausal women decreased first and then increased with age, and were negatively correlated with bone mineral density of lumbar vertebrae, femoral neck and total hip. The serum 25 (OH) VitD level of postmenopausal women in Beijing was significantly lower than that of postmenopausal women (13.10 鹵5.37ng/ml). There was no correlation between 尾-CTX P1NP and 25 (OH) VitD and vertebral fracture. Conclusion: MATN3 gene polymorphism may affect the variation of total hip bone density in postmenopausal women for the first time, but has no correlation with bone turnover markers and vitamin D levels.
【學位授予單位】：北京協(xié)和醫(yī)學院
【學位級別】：博士
【學位授予年份】：2010
【分類號】：R580;R3416
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