發(fā)布時間：2018-07-23 16:11

【摘要】： 目的:探索應(yīng)用三氯化鐵溶液外敷聯(lián)合夾閉動脈建立兔股動脈血栓模型的方法。方法:選取新西蘭大白兔21只,將其雙側(cè)股動脈隨機分為:聯(lián)合法夾閉10分鐘組(A組)、聯(lián)合法夾閉7分鐘組(B組)、聯(lián)合法夾閉5分鐘組(C組)、聯(lián)合法夾閉3分鐘組(D組)、外敷對照組(E組)、夾閉對照組(F組),每組7條股動脈。所有聯(lián)合法組在10%三氯化鐵溶液外敷的基礎(chǔ)上分別給予夾閉股動脈10、7、5、3分鐘;外敷對照組(E組):給予10%三氯化鐵溶液股動脈外敷;夾閉對照組(F組):夾閉股動脈10分鐘。用多普勒血流儀連續(xù)監(jiān)測股動脈的血流量,同時留取股動脈的二維及彩色多普勒超聲圖像,1小時為實驗終點,實驗結(jié)束對實驗段股動脈進行病理分析。兔股動脈血栓模型成功建立的標(biāo)準(zhǔn)為閉塞性血栓形成。結(jié)果:聯(lián)合法夾閉10分鐘組(A組)、聯(lián)合法夾閉7分鐘組(B組)、聯(lián)合法夾閉5分鐘組(C組)、聯(lián)合法夾閉3分鐘組(D組)建模成功率均分別為100%、100%、71%、29%,所形成的血栓均為富含血小板的混合型血栓;外敷對照組(E組)和夾閉對照組(F組),1小時內(nèi)均未形成閉塞性血栓。結(jié)論:三氯化鐵溶液外敷聯(lián)合夾閉動脈建立兔股動脈血栓模型的方法,所需三氯化鐵溶液濃度較低,血栓形成率高,是一種較實用的血栓模型制作方法。
[Abstract]:Aim: to explore the method of establishing rabbit femoral artery thrombosis model by external application of ferric chloride solution combined with clipping artery. Methods: 21 New Zealand white rabbits were selected. The bilateral femoral arteries were randomly divided into three groups: combined occlusion for 10 minutes (group A), combined occlusion for 7 minutes (group B), combined occlusion for 5 minutes (group C), combined occlusion for 3 minutes (group D) and external application for control group (group E). In control group (group F), there were 7 femoral arteries in each group. On the basis of external application of 10% ferric chloride solution, all the combined treatment groups were given 107U 5min clamping femoral artery for 3 minutes respectively, group E: 10% ferric chloride solution for external application of femoral artery, and group F clamping control group (group F): clamping femoral artery for 10 minutes. The blood flow of femoral artery was continuously monitored by Doppler flow meter, and 2-D and color Doppler ultrasound images of femoral artery were taken for 1 hour as the end point of the experiment. The pathological analysis of the femoral artery was carried out at the end of the experiment. The standard of successful establishment of rabbit femoral artery thrombosis model is occlusion thrombosis. Results: the success rates of modeling in group A (group A), group B (group B), group C (group C), group C (group C) and group D (group D) were 100% and 100%, respectively. Mixed thrombus containing platelets; No occlusive thrombus was found in the control group (E group) and clamped control group (F group) within 1 hour. Conclusion: ferric chloride solution is a practical method for the establishment of rabbit femoral artery thrombosis model by external application of ferric chloride solution combined with occlusion of artery. The concentration of ferric trichloride solution is lower and the rate of thrombosis is high.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R-332

【引證文獻】

相關(guān)碩士學(xué)位論文 前1條

1 盛書娟;石榴皮提取物對血細(xì)胞生理特性的影響[D];山東師范大學(xué);2012年

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本文編號：2139939