【摘要】： 研究目的 研究妊娠不同時(shí)期胚胎小鼠皮膚表面神經(jīng)末梢發(fā)育規(guī)律,以及其與羊水接觸的接觸情況,探討其與羊水的相互關(guān)系,分析羊水中具體物質(zhì)對于皮膚表皮表面神經(jīng)發(fā)育的作用。 研究方法 分別取胚胎12、13、14、15、16、17 d小胚鼠,冰凍切片,行神經(jīng)特異性標(biāo)記物NF免疫組織化學(xué)染色,在光鏡下觀察皮膚表面神經(jīng)末梢的分布情況并攝片;為了進(jìn)一步闡明胚胎期小鼠皮膚神經(jīng)末梢是否與羊水接觸,將熒光金在體注射到胚胎羊水中,切片后用熒光顯微鏡觀察神經(jīng)元被標(biāo)記情況。 研究結(jié)果 胚胎期小鼠表皮表面存在有大量的神經(jīng)末梢,胚胎第12d已經(jīng)可以清晰看到,以胚胎第15 d最多。在胚胎的背根神經(jīng)節(jié),神經(jīng)根及腹背側(cè)灰質(zhì)中均發(fā)現(xiàn)有明顯的熒光標(biāo)記。 研究結(jié)論 胚胎期小鼠皮膚神經(jīng)末梢與羊水有一定的接觸關(guān)系。胚胎時(shí)期小鼠皮膚神經(jīng)末梢伸入羊水中,與羊水直接接觸,可以感知羊水成分的變化,或者是向羊水中釋放某些物質(zhì),但是羊水成分中何種物質(zhì)對于周圍神經(jīng)生長起作用以及其起作用的具體機(jī)制現(xiàn)在尚不明了,需進(jìn)一步探討。
[Abstract]:Objective to study the development rule of skin surface nerve endings in different stages of pregnancy and its contact with amniotic fluid, and to explore the relationship between them and amniotic fluid. To analyze the effect of specific substances in amniotic fluid on the development of skin epidermal nerve. Methods the embryonic mice were collected from 12D embryo, frozen sections were used for NF immunohistochemical staining, the distribution of nerve endings on the skin surface was observed under light microscope, and the results were taken by radiograph. The distribution of the nerve endings on the surface of the skin was observed under light microscope, and the distribution of the nerve endings on the surface of the skin was also observed under light microscope. In order to further elucidate whether the skin nerve endings of embryonic mice are in contact with amniotic fluid, fluorescent gold was injected into embryonic amniotic fluid in vivo, and the labeling of neurons was observed by fluorescence microscope. The results showed that there were a lot of nerve endings on the surface of mouse epidermis at embryonic stage, which could be seen clearly on the 12th day of embryo, and the highest number was on the 15th day of embryo. Obvious fluorescent labeling was found in dorsal root ganglion, nerve root and ventral dorsal gray matter of embryo. Conclusion there is a certain contact relationship between skin nerve endings and amniotic fluid in embryonic mice. At embryonic stage, the skin nerve endings of mice extend into amniotic fluid and contact with amniotic fluid directly, which can sense the changes of amniotic fluid composition, or release certain substances into amniotic fluid. However, it is not clear which substances in amniotic fluid play an important role in the growth of peripheral nerve and the mechanism of its action.
【學(xué)位授予單位】：江蘇大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R321

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