本文選題：大鼠 + 實驗性精索靜脈曲張　； 參考：《中國協(xié)和醫(yī)科大學》2008年博士論文

【摘要】： 第一部分:大鼠實驗性精索靜脈曲張模型的建立及其可行性評價 【目的】探討成功建立實驗性左側(cè)精索靜脈曲張動物模型的方法,研究精索靜脈曲張大鼠對睪丸的損害,評價模型用于實驗研究的可行性,探討精索靜脈曲張不育的機理。 【方法】通過部分結扎左腎靜脈建立大鼠實驗性精索靜脈曲張(ELV)模型,分別于術后6周和9周和12周研究雙側(cè)睪丸的組織學改變并進行統(tǒng)計學分析。 【結果】該模型大鼠的睪丸病理損害以生精上皮退化,精子發(fā)生阻滯,生精細胞脫落,曲精小管萎縮,間質(zhì)水腫最為多見,損害為雙側(cè)性,統(tǒng)計學分析顯示,ELV大鼠左右側(cè)睪丸曲細精管退化百分比較對照組增加,病變有時間累進性特點,與人類睪丸的病理損害具有相似性。 【結論】成功建立了ELV模型,該模型為研究精索靜脈曲張的病理生理改變提供了有用工具。ELV大鼠雙側(cè)睪丸有嚴重的累進性病理改變,這可能是精索靜脈曲張不育的主要原因之一。 第二部分:左側(cè)精索靜脈曲張與腎上腺代謝產(chǎn)物返流 【目的】在成功建立大鼠實驗性左側(cè)精索靜脈曲張模型的基礎上,探討實驗性大鼠左側(cè)精索靜脈曲張與腎上腺代謝產(chǎn)物返流的關系,進一步探討腎上腺代謝產(chǎn)物返流在精索靜脈曲張不育發(fā)生機理中的作用。 【方法】10只成年雄性SD大鼠,每只體重量為270—300克,隨機分為2組,每組5只,A組通過部分結扎腎靜脈建立實驗性精索靜脈曲張模型;B組在建立左側(cè)精索靜脈曲張模型的基礎上,結扎腎上腺靜脈,模型建立6周后觀察二組模型大鼠腎上腺靜脈直徑、腎臟和腎上腺病理變化;并比較大鼠和人類腎上腺靜脈解剖,了解二者之間的相似性。 【結果】A組大鼠腎臟體積無明顯變化,成功建立了ELV大鼠模型,B組大鼠腎臟全部萎縮,B組腎上腺靜脈與A組相比明顯增粗,二組比較具有統(tǒng)計學意義。二組大鼠腎上腺病理比較無明顯差異。大鼠腎上腺靜脈解剖與人類相比,有很大的相似性,除腎上腺靜脈以外,存在膈下靜脈交通。 【結論】實驗性精索靜脈曲張大鼠腎上腺靜脈成為腎臟靜脈血流分支之一,不存在腎上腺代謝產(chǎn)物返流;腎上腺代謝產(chǎn)物返流不是精索靜脈曲張不育的原因。 第三部分:Notch 1蛋白在正常和左側(cè)精索靜脈曲張模型大鼠睪丸中的表達及其意義 【目的】探討Notch 1蛋白在正常和左側(cè)精索靜脈曲張模型大鼠睪丸中的表達及其在精子發(fā)生過程中的作用,進一步了解Notch 1在精索靜脈曲張不育發(fā)生機理中的作用。 【方法】應用第一部分中成功建立的大鼠ELV模型睪丸標本(假手術組、6周組、9周組、12周組)一部分應用于免疫組化研究,凍存部分應用Westenbloting方法檢測Notch 1表達水平。采用單因素方差分析和顯著性檢驗進行統(tǒng)計學分析。 【結果】在睪丸生精小管中,Notch 1主要表達于精原細胞和初級精母細胞胞質(zhì)內(nèi),而在支持細胞內(nèi)未見陽性表達;精原細胞表達為強陽性。精索靜脈曲張引起Notch 1在雙側(cè)睪丸中表達減少,并有時間累進性的特點,組間比較具有統(tǒng)計學意義。 【結論】Notch 1蛋白可能參與精原細胞分化,增值過程,ELV引起累進性Notch 1表達減少,可能是VC導致不育的重要原因之一。
[Abstract]:Part one: establishment of rat experimental varicocele model and its feasibility evaluation.
[Objective] to explore the method of establishing experimental left spermatic varicocele animal model, study the damage of spermatic varicose rat to testis, evaluate the feasibility of the model in experimental study, and explore the mechanism of sterility of varicocele.
[Methods] the experimental varicocele (ELV) model of rats was established by partial ligation of the left renal vein. The histological changes of bilateral testicles were studied at 6, 9 and 12 weeks after the operation, and the statistical analysis was performed.
[results] the pathological damage of the rat testis in this model was the degeneration of the spermatogenic epithelium, the block of spermatogenesis, the exfoliation of spermatogonia, the atrophy of the seminiferous tubules, the most common interstitial edema, and the bilateral damage. The statistical analysis showed that the degeneration of the testicular seminiferous tubules in the left and right sides of the ELV rats was increased compared with the control group, and the pathological changes had time progressive characteristics, The pathological damage of human testicles is similar.
[Conclusion] the ELV model has been successfully established. This model provides a useful tool for the study of the pathophysiological changes of varicocele. The bilateral testicles of.ELV rats have serious progressive pathological changes, which may be one of the main reasons for the sterility of varicocele.
The second part: left varicocele and adrenal metabolite reflux.
[Objective] to explore the relationship between the left spermatic varicocele and adrenalic reflux in experimental rats on the basis of the successful establishment of experimental left spermatic varicocele model in rats, and to further explore the role of adrenalic reflux in the mechanism of spermatic varicocele infertility.
[Methods] 10 adult male SD rats, each body weight of 270 to 300 grams, were randomly divided into 2 groups, each group was 5. The A group established the experimental varicocele model by partial ligature of the renal vein. Group B was established on the basis of the left spermatic varicocele model and ligated the adrenal vein, and the model was established to observe the adrenal adrenal gland of two groups of rats after 6 weeks. The diameter of vein, the pathological changes of kidney and adrenal glands, and the anatomy of the adrenal veins between rats and human beings were compared to understand the similarity between the two.
[results] there was no obvious change in kidney volume in the A group. The ELV rat model was successfully established. The kidneys of group B rats were all atrophied. The adrenal vein in group B was significantly thicker than that in A group. The two groups were statistically significant. There was no significant difference in the pathology of adrenal glands in the two groups. The anatomy of the suprarenal gland vein was similar to that of the human. In addition to the adrenal vein, there is a subphrenic vein traffic.
[Conclusion] the adrenal vein of experimental varicocele is one of the branches of renal venous blood flow, and there is no reflux of adrenalic metabolites, and adrenalic reflux is not the cause of sterility of varicocele varicocele.
The third part: the expression and significance of Notch 1 protein in the testis of normal and left varicocele rats.
[Objective] to investigate the expression of Notch 1 protein in the testis of normal and left spermatic varicocele model rats and its role in the process of spermatogenesis, and to further understand the role of Notch 1 in the mechanism of sterility of varicocele.
[Methods] the testicular specimens of rat ELV model (sham operation group, 6 week group, 9 week group, 12 week group) were applied to immunohistochemical study in part one, and the expression level of Notch 1 was detected by Westenbloting method. The statistical analysis was carried out by single factor analysis of variance and significant test.
[results] in the testicular seminiferous tubules, Notch 1 was mainly expressed in the cytoplasm of spermatogonial and primary spermatocyte, but no positive expression in the supporting cells. The expression of spermatogonial cells was strong positive. The expression of Notch 1 in the spermatic vein decreased in bilateral testicles and had the characteristics of progressive time. The comparison of the groups was statistically significant.
[Conclusion] Notch 1 protein may be involved in the differentiation of spermatogonial cells, the increment process, and the decrease of progressive Notch 1 expression caused by ELV, which may be one of the important reasons for the infertility caused by VC.
【學位授予單位】：中國協(xié)和醫(yī)科大學
【學位級別】：博士
【學位授予年份】：2008
【分類號】：R697.24;R-332;R698.2

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