本文選題：26RFa + C末端片段　； 參考：《蘭州大學(xué)》2009年碩士論文

【摘要】： 26RFa是新發(fā)現(xiàn)的RFamide結(jié)尾的相關(guān)肽,其內(nèi)源性受體為GPR103。結(jié)構(gòu)分析發(fā)現(xiàn)26RFa C末端八肽高度保守,并且中間存在螺旋結(jié)構(gòu)引領(lǐng)著N-末端和C-末端。已有的研究表明26RFa相關(guān)肽能介導(dǎo)多種生理活性,主要包括攝食,能量消耗,運(yùn)動,內(nèi)分泌,骨生成和痛覺調(diào)節(jié)。但現(xiàn)在對26RFa生理活性研究的還不全。本論文主要選擇26RFa、C-末端片段26RFa(19-26),26RFa(8-26)作為GPR103受體激動劑來探討他們對大鼠心血管系統(tǒng)影響和小鼠痛覺系統(tǒng)影響及其作用機(jī)制。 研究表明靜脈注射26RFa(100-800 nmol/kg,i.v.)可以使麻醉的大鼠血壓雙向變化并增加心率,升血壓和心率過程依賴兒茶酚胺機(jī)制;側(cè)腦室注射大鼠/小鼠26RFa(5,10 and 20nmol)有明顯的鎮(zhèn)痛作用,是通過抑制下行γ-氨基丁酸能神經(jīng)元起作用,與阿片系統(tǒng)無關(guān)。26RFa的兩個C-末端片段26RFa(8-26)和26RFa(19-26)在外周心血管活性和脊髓水平上鎮(zhèn)痛調(diào)節(jié)等方面的作用可能是通過與26RFa作用途徑不同的其它機(jī)制起作用的,從而暗示了N-末端對于26RFa生物活性的維持可能起著重要作用。
[Abstract]:26RFa is a newly discovered peptide associated with the end of RFamide, and its endogenous receptor is GPR103. Structural analysis showed that the C-terminal octapeptide of 26RFa was highly conserved, and there was a helical structure in the middle leading the N-terminal and C-terminal. Previous studies have shown that 26RFa-related peptides mediate a variety of physiological activities, including food intake, energy expenditure, exercise, endocrine, bone formation and pain regulation. However, the physiological activity of 26RFa has not been fully studied. In this study, we selected 26RFa (19-26) 26RFa (8-26) as the agonist of GPR103 receptor to investigate their effects on the cardiovascular system in rats and the pain system in mice and its mechanism. The results showed that 26RFa (100-800 nmol / kg i.v.) was injected intravenously. The changes of blood pressure in anesthetized rats were bidirectional and increased heart rate. The mechanism of ascending blood pressure and heart rate was dependent on catecholamine mechanism, and intracerebroventricular injection of 26RFa (5 ~ 10 and 20nmol) had obvious analgesic effect. By inhibiting downlink 緯 -aminobutyric acid neurons, The effects of two C-terminal fragments 26RFa (8-26) and 26RFa (19-26) unrelated to the opioid system on peripheral cardiovascular activity and analgesic regulation at spinal cord level may be mediated by other mechanisms different from those of 26RFa. This suggests that N- terminal may play an important role in the maintenance of 26RFa biological activity.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R33

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1 劉謙;26RFa及其C末端片段在心血管和痛覺方面活性研究[D];蘭州大學(xué);2009年

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本文編號：2089130