本文選題：百草枯中毒 + 熱休克蛋白70　； 參考：《中國醫(yī)科大學》2010年碩士論文

【摘要】： HSP70在百草枯誘導的肺部炎癥反應中的表達 實驗目的 本研究將應用病理HE染色方法檢測百草枯中毒大鼠肺組織病理變化及病理損傷程度,應用免疫組織化學及Western blot方法檢測百草枯中毒大鼠肺組織中熱休克蛋白(HSP70)的表達水平。探索百草枯致大鼠急性肺損傷的發(fā)生機制,對臨床工作中百草枯中毒患者早期的救治提供有利的線索。 實驗方法 72只健康Wistar大鼠隨機平均分成3組,分別腹腔注射生理鹽水(A組)、20 mg/kg百草枯(B組)和40 mg/kg百草枯(C組)。各組動物1、2、3、6、12和24 h隨機編入6個觀察時點。造模前,各組大鼠禁食12 h,不禁水。A組大鼠腹腔注射1 ml生理鹽水,B組和C組大鼠用生理鹽水將PQ稀釋至1 ml腹腔注射染毒。每組分別于6個觀察時點用10％水合氯醛腹腔注射后處死4只大鼠,留取肺組織標本,左側肺組織置于4％多聚甲醛中固定12 h,用于HE染色,免疫組織化學染色。取右側肺組織放于-70℃冰箱備用,Western blot方法測定肺組織中HSP70的表達。 實驗結果 HE染色,染毒大鼠肺組織發(fā)生急性肺損傷、透明膜的形成和炎性細胞浸潤等急性肺損傷典型的病理改變。免疫組織化學結果,B組中HSP70的表達于2 h開始升高,6 h達到峰值,24 h與A組無差別。C組中HSP70的表達于1 h開始升高,3 h達到峰值,24 h與A組無差別。且C組2、3和12 h的表達均顯著高于B組。Western blot結果,B組大鼠2、3、6 h時點HSP70的表達在肺組織中較A組明顯增強,其他時間點與A組比無明顯差異。C組1、2、3、6、12 h時點HSP70的表達較A組均明顯增強,2、3、12 h時點HSP70的表達較B組明顯增強。 結論 百草枯誘導的急性肺損傷可引起支氣管、細支氣管和肺泡上皮細胞HSP70應激性表達,中毒劑量增大,肺損傷加重,HSP70的表達亦隨之增強,而肺組織的病理損傷卻沒有顯著性差異,提示HSP70對肺損傷起保護作用。
[Abstract]:Expression of HSP70 in paraquat induced pulmonary inflammation objective to study the pathological changes and degree of lung injury in paraquat poisoned rats by pathological HE staining. The expression of heat shock protein (HSP70) in the lung tissue of paraquat poisoned rats was detected by immunohistochemistry and Western blot. To explore the mechanism of acute lung injury induced by paraquat in rats and to provide a useful clue for the early treatment of paraquat poisoning in clinical work. Methods Seventy-two healthy Wistar rats were randomly divided into 3 groups and received intraperitoneal injection of normal saline (group A) for 20 mg/kg paraquat (group B) and 40 mg/kg paraquat (group C). The animals in each group were randomly assigned to 6 observation time points at 12 and 24 hours. Before the model was established, rats in each group fasted for 12 hours. Rats in group A were injected intraperitoneally with 1 ml normal saline and rats in group C diluted PQ to 1 ml by intraperitoneal injection. Four rats were killed after intraperitoneal injection of 10% chloral hydrate at 6 observation points in each group. The left lung tissue was fixed in 4% paraformaldehyde for 12 hours and was used for HE staining and immunohistochemical staining. The expression of HSP70 in lung tissue was determined by Western blot method. The results showed that acute lung injury, hyaline membrane formation and inflammatory cell infiltration were typical pathological changes in lung tissue of rats exposed to HE. Immunohistochemical results showed that the expression of HSP70 in group B began to increase at 2 h and reached a peak at 6 h. There was no difference in HSP70 expression between group A and group A. the expression of HSP70 in group C increased at 1 h and reached peak value at 3 h. There was no difference between group A and group A at 24 h. The expression of HSP70 in group C was significantly higher than that in group B at 2h and 12h. The results of Western blot showed that the expression of HSP70 in group B was significantly higher than that in group A. There was no significant difference between other time points and A group. The expression of HSP70 in group C was significantly higher than that in group A at 12 h after 12 h. The expression of HSP70 in group C was significantly higher than that in group B at 12 h. Conclusion the acute lung injury induced by paraquat can induce the stress expression of HSP70 in bronchioles, bronchioles and alveolar epithelial cells. However, there was no significant difference in pathological injury of lung tissue, suggesting that HSP70 plays a protective role in lung injury.
【學位授予單位】：中國醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R363

【參考文獻】

相關期刊論文 前6條

1 田英平;邱澤武;;百草枯中毒的救治[J];中國實用內科雜志;2007年15期

2 謝東輝;章輝;鄭蘭榮;;烏司他丁對百草枯中毒全身炎癥反應綜合征治療作用及其機制的研究[J];實用醫(yī)學雜志;2008年08期

3 彭曉東;陳驥;梁創(chuàng);;急性百草枯中毒后MODS患者血清TNF-α和IL-10的變化及意義[J];中國實用醫(yī)藥;2008年14期

4 魯新;郭偉;劉航星;;血液灌流串聯(lián)血液透析治療百草枯中毒臨床觀察[J];中國誤診學雜志;2006年04期

5 ;Correlation between clinicopathology and expression of heat shock protein 70 and glucose-regulated protein 94 in human colonic adenocarcinoma[J];World Journal of Gastroenterology;2005年07期

6 張立辰;趙宏霞;;血液灌流聯(lián)合血液透析治療急性百草枯中毒臨床觀察[J];中國中西醫(yī)結合急救雜志;2008年04期

，

本文編號：2079949