本文選題：脾臟注射 + 樹突狀細(xì)胞　； 參考：《南京醫(yī)科大學(xué)》2009年碩士論文

【摘要】：目的:研究經(jīng)脾臟注射骨髓來源的未成熟樹突狀細(xì)胞(iDC)對(duì)大鼠小腸移植免疫耐受的誘導(dǎo)。 方法:分離供體F344大鼠骨髓來源DC細(xì)胞前體,經(jīng)GM-CSF、IL-4體外刺激培養(yǎng)6天后,檢測(cè)細(xì)胞表面共刺激分子及MHC表達(dá),鑒定是否為imDC;健康成年F344大鼠為供體,SD大鼠為受體。隨機(jī)分為四組(n=10),A組(陰性對(duì)照組):移植前7天,受體經(jīng)陰莖背靜脈注射生理鹽水1ml;B組(陰性對(duì)照組):移植前7天,受體經(jīng)脾臟注射生理鹽水1ml;C組(iDC陰莖背靜脈注射組):移植前7天,受體經(jīng)陰莖背靜脈注射供體的iDC,細(xì)胞數(shù)為2×106個(gè);D組(iDC脾臟注射組):移植前7天,受體經(jīng)脾臟注射供體的iDC,細(xì)胞數(shù)為2×106個(gè)。四組大鼠注射后1周后行大鼠異位節(jié)段性小腸移植術(shù)。觀察:1、大鼠移植后存活情況;2、移植后7天移植腸病理學(xué)情況;3、同時(shí)TUNNEL法檢測(cè)移植腸的細(xì)胞凋亡情況。 結(jié)果: D組大鼠移植小腸存活時(shí)間為(17.50±1.05)d,較A組(7.17±1.17)d、B組(7.33±1.51)d、C組(12.83±2.79)d顯著延長(zhǎng);D組移植小腸炎性細(xì)胞浸潤(rùn)、黏膜組織結(jié)構(gòu)破壞程度明顯低于A、B、C組;D組小腸粘膜上皮細(xì)胞凋亡數(shù)(9.3±1.7)個(gè),相對(duì)于A組(25.3±4.6)個(gè)、B組(25.5±4.7)個(gè)、C組(16.5±2.1)個(gè),移植腸粘膜上皮細(xì)胞凋亡數(shù)顯著降低。 結(jié)論:經(jīng)脾臟注射供體來源的未成熟樹突狀細(xì)胞,可在一定程度上誘導(dǎo)受體產(chǎn)生免疫耐受,延長(zhǎng)受體大鼠小腸移植術(shù)后的存活時(shí)間。D組移植小腸炎性細(xì)胞浸潤(rùn)、黏膜組織結(jié)構(gòu)破壞程度和黏膜細(xì)胞的凋亡數(shù)目明顯低于A、B、C組。
[Abstract]:Aim: to study the induction of immune tolerance of rat small bowel transplantation by immature dendritic cells (iDC) injected into spleen. Methods: DC cells derived from bone marrow of donor F344 rats were isolated and cultured in vitro for 6 days. The expression of costimulatory molecules and MHC on the surface of the cells was detected to determine whether it was imDC.The healthy adult F344 rats were used as donors and SD rats as the receptors. They were randomly divided into four groups: group A (negative control group): 7 days before transplantation, 1 ml of normal saline was injected into the dorsal vein of penis (negative control group): 7 days before transplantation, 1 ml of normal saline was injected into the dorsal vein of penis in group B (negative control group). The recipient was injected with 1 ml saline into the spleen (IDC group): 7 days before transplantation, the recipient was injected intravenously into the dorsal penile vein of the donor, the number of cells was 2 脳 106 D group (iDC splenic injection group): 7 days before transplantation, the recipient was injected into the dorsalis penile vein, and the number of cells was 2 脳 106 D group (iDC splenic injection group), 7 days before transplantation. The number of iDCs injected into spleen was 2 脳 106. Four groups of rats were treated with ectopic segmental small bowel transplantation 1 week after injection. The survival and pathology of grafted intestine were observed at 7 days after transplantation. The apoptosis of transplanted intestine was detected by Tunel method. Results: the survival time of small intestine transplantation in group D was (17.50 鹵1.05) days, which was significantly longer than that in group A (7.17 鹵1.17) d, group C (7.33 鹵1.51) d (12.83 鹵2.79) days, and the degree of mucosal structure damage was significantly lower than that in group A (9.3 鹵1.7). Compared with group A (25.3 鹵4.6), group B (25.5 鹵4.7) and group C (16.5 鹵2.1), the number of apoptotic cells decreased significantly. Conclusion: injection of donor derived immature dendritic cells into the spleen can induce immune tolerance to some extent and prolong the survival time of the recipient rats after small bowel transplantation. Group D can induce the infiltration of inflammatory cells in the transplanted small intestine. The degree of damage of mucosal structure and the number of apoptosis of mucosal cells were significantly lower than those of group A.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R392

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