本文選題：α-硫辛酸 + 脂肪因子��； 參考：《檢驗(yàn)醫(yī)學(xué)》2015年11期

【摘要】：目的探討α-硫辛酸(ALA)對(duì)飲食誘導(dǎo)肥胖大鼠脂肪因子的影響。方法選取清潔級(jí)8周齡雄性Wistar大鼠50只,隨機(jī)選其中10只,以普通飼料喂養(yǎng)作為對(duì)照組(NC);高脂飲食成功誘導(dǎo)肥胖大鼠模型32只,隨機(jī)分為高脂飼料(HFD)組(16只)和HFD+ALA組[16只,腹腔注射ALA(30 mg/kg)2周]。測(cè)定各組大鼠血清氧化應(yīng)激、脂肪因子等指標(biāo)并進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果 HFD組血清丙二醛(MDA)、瘦素、腫瘤壞死因子-α(TNF-α)分別為(8.8±0.7)μmol/L、(9.8±0.8)ng/m L、(11.6±1.2)ng/L,明顯高于HFD+ALA組[(6.1±0.6)μmol/L、(7.6±0.7)ng/m L、(9.7±2.3)ng/L]和NC組[(7.9±0.4)μmol/L、(4.6±0.6)ng/m L、(8.8±3.5)ng/L](P均0.05);而超氧化物歧化酶(SOD)[(73.9±5.1)U/m L]、總抗氧化能力(T-AOC)[(18.0±3.6)U/m L]、脂聯(lián)素[(8.7±1.5)mg/L]明顯低于HFD+ALA組[(80.9±1.8)U/m L、(25.8±5.4)U/m L、(12.6±2.1)mg/L]和NC組[(81.0±3.2)U/m L、(20.9±2.3)U/m L、(14.0±3.4)mg/L](P均0.05)。與NC組比較,HFD+ALA組血清T-AOC、瘦素明顯升高(P均0.05),MDA明顯下降(P0.05),SOD、脂聯(lián)素、TNF-α差異無(wú)統(tǒng)計(jì)學(xué)意義(P均0.05)。相關(guān)性分析顯示血清MDA與瘦素、TNF-α呈明顯正相關(guān)[相關(guān)系數(shù)(r)值分別為0.716、0.502,P均0.01];T-AOC與脂聯(lián)素呈負(fù)相關(guān)(r=-0.485,P0.05)。結(jié)論 ALA可改善肥胖大鼠的氧化應(yīng)激狀態(tài)并影響脂肪因子的分泌。
[Abstract]:Objective to investigate the effect of 偽-lipoic acid (Ala) on fat factors in obese rats induced by diet. Methods Fifty eight week-old male Wistar rats of clean grade were randomly selected, 10 of them were fed with common diet as control group (NC), 32 rats were successfully induced obesity by high-fat diet, and were randomly divided into high-fat diet (HFD) group (16 rats) and HFD ALA group (16 rats). Ala (30 mg/kg) was injected intraperitoneally for 2 weeks. The indexes of serum oxidative stress and fat factor were measured and analyzed statistically. Results in HFD group, serum malondialdehyde (MDA), leptin, 鑲跨槫鍧忔鍥犲瓙-偽(TNF-偽)鍒嗗埆涓，

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