本文選題：脊髓 + 衰老��； 參考：《山東大學(xué)》2009年碩士論文

【摘要】： 目的:應(yīng)用放射性配基結(jié)合試驗(yàn),探討自然衰老過程中脊髓突觸體外周型苯二氮卓受體(PBRs)的動態(tài)變化以及與外周血血小板膜PBRs的相關(guān)性。 方法:實(shí)驗(yàn)動物SD大鼠(雌雄各半)分為3月齡和24月齡2組。動物斷頭處死后,迅速取脊髓。采用梯度離心技術(shù)制備脊髓突觸體,低滲溶血法制備外周血血小板膜。應(yīng)用放射配基[~3H]PK11195結(jié)合實(shí)驗(yàn)測定PBRs結(jié)合活力。 結(jié)果:各組動物雄性、雌性間比較脊髓突觸體、外周血血小板膜PBRs結(jié)合活性差異均無顯著意義(P＞0.05)。隨著鼠齡增加,脊髓突觸體及外周血血小板膜[~3H]PK11195結(jié)合活性顯著下降。外周血血小板膜[~3H]PK11195結(jié)合活性與脊髓突觸體[~3H]PK11195結(jié)合活性間具有顯著相關(guān)性(r=0.985,P＜0.001)。 結(jié)論:脊髓突觸體PBRs水平呈增齡下降改變,外周血血小板膜[~3H]PK11195結(jié)合活性能夠反映脊髓組織該指標(biāo)的變化。
[Abstract]:Aim: to investigate the dynamic changes of peripheral benzodiazepine receptor (PBRs) in spinal cord synapses during natural senescence and the correlation between PBRs and PBRs in peripheral blood platelets. Methods: Sprague-Dawley rats (male and female) were divided into 2 groups: 3 months old and 24 months old. After the animals were killed, the spinal cord was quickly removed. Spinal synaptosomes were prepared by gradient centrifugation and platelet membranes were prepared by hypotonic hemolysis. The binding activity of PBRs was determined by radioligand [3 H] PK11195 binding assay. Results: there was no significant difference in PBRs binding activity between male and female spinal cord synaptosomes and peripheral blood platelets (P > 0.05). The binding activity of PK11195 in synaptosomes of spinal cord and platelets in peripheral blood decreased significantly with aging. There was a significant correlation between the binding activity of PK11195 in peripheral blood platelet membrane and the binding activity of PK11195 in spinal synaptosomes (r = 0.985, P < 0.001). Conclusion: the level of PBRs in spinal synaptosomes decreased with age, and the binding activity of [3H] PK11195 in peripheral blood platelets could reflect the changes in spinal cord tissues.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R33

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 陳春富;郎森陽;左萍萍;王湘慶;夏程;楊楠;;血小板膜與腦細(xì)胞線粒體和突觸體外周型苯二氮卓受體的相關(guān)性及生物學(xué)意義[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2006年04期

2 唐珉;李剛;蔡偉;肖俐;;青年和老年大白鼠脊髓運(yùn)動神經(jīng)細(xì)胞突觸的觀察[J];江西醫(yī)學(xué)院學(xué)報;2008年03期

，

本文編號：2072485