本文選題：大鼠 + Inhibinα蛋白　； 參考：《華中科技大學(xué)》2009年碩士論文

【摘要】：目的:確定雄性大鼠Inhibinα亞基的mRNA及蛋白在各種組織中有無表達(dá)及相對表達(dá)量。 方法:選用成年SD雄性大鼠,10%水合氯醛麻醉后處死,立即取睪丸、附睪、腎、腎上腺及肝組織,采用免疫組化法和熒光定量PCR方法分別檢測Inhibinα蛋白和mRNA在大鼠各組織中的表達(dá)情況。 結(jié)果:Inhibinα蛋白在睪丸、附睪、腎、腎上腺組織中均有表達(dá),在肝臟組織中無表達(dá)。InhibinαmRNA在各組織中的表達(dá)相對量依次為:腎腎上腺睪丸附睪。 結(jié)論:本實(shí)驗(yàn)從定性和定量兩方面檢測了Inhibinα亞基的表達(dá)情況,結(jié)果提示Inhibinα亞基不僅僅在生殖系統(tǒng)有表達(dá),在其它系統(tǒng)也有表達(dá)。 目的:將大鼠Inhibinα基因的啟動(dòng)子區(qū)分段克隆后分別構(gòu)建真核表達(dá)載體,為進(jìn)一步篩選出其核心啟動(dòng)子做好準(zhǔn)備。 方法:利用已有文獻(xiàn)及網(wǎng)絡(luò)資源進(jìn)行Inhibinα啟動(dòng)子區(qū)的預(yù)測及生物信息學(xué)分析,用基因重組技術(shù)將預(yù)測的啟動(dòng)子區(qū)分段克隆到真核表達(dá)載體pEGFP-N1中,并對其測序驗(yàn)證。 結(jié)果:成功克隆了大鼠Inhibinα基因的啟動(dòng)子區(qū)的395bp、606bp、775bp大小的片段,并分別構(gòu)建了這三種片段的真核表達(dá)載體。 結(jié)論:對預(yù)測的大鼠Inhibinα基因的啟動(dòng)子區(qū)分段克隆,并成功構(gòu)建了含有這些克隆片段的真核表達(dá)載體。
[Abstract]:Aim: to determine the mRNA and protein expression of Inhibin 偽 subunit in various tissues of male rats. Methods: adult SD male rats were killed after anaesthesia with 10% chloral hydrate. Testis, epididymis, adrenal gland and liver tissues were collected immediately. The expression of Inhibin 偽 protein and mRNA in rat tissues was detected by immunohistochemistry and fluorescence quantitative PCR, respectively. Results the percent Inhibin 偽 protein was expressed in testis, epididymis and adrenal gland. Conclusion: the expression of Inhibin 偽 subunit was detected qualitatively and quantitatively in this experiment. The results suggest that the expression of Inhibin 偽 subunit is not only expressed in reproductive system, but also in other systems. Aim: to construct the eukaryotic expression vector of rat Inhibin 偽 gene promoter after cloning, so as to prepare for the further screening of its core promoter. Methods: the predicted promoter differentiation segment was cloned into the eukaryotic expression vector pEGFP-N1 by gene recombination technique, and was sequenced and verified by using the existing literature and network resources to predict the promoter region of Inhibin 偽 and bioinformatics analysis. Results: the promoter region of rat Inhibin 偽 gene was successfully cloned and cloned, and the eukaryotic expression vectors of these three fragments were constructed. Conclusion: the promoter differentiation cloning of the predicted rat inhibin 偽 gene was carried out and the eukaryotic expression vector containing these fragments was successfully constructed.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號(hào)】：R346

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