本文選題：莪術(shù) + 局灶性腦缺血�。� 參考：《廣西醫(yī)科大學(xué)學(xué)報(bào)》2015年06期

【摘要】：目的:探討莪術(shù)對(duì)局灶性腦缺血模型大鼠的神經(jīng)保護(hù)作用及機(jī)制。方法:健康成年雄性SD大鼠60只,隨機(jī)分為6組,分別為假手術(shù)對(duì)照組、模型對(duì)照組、尼莫地平組、莪術(shù)高、中、低劑量組。除了假手術(shù)對(duì)照組,其余組大鼠采用線栓法制作大鼠大腦中動(dòng)脈梗阻模型。造模前預(yù)防給藥7d,造模后給藥3d,每天1次。各組大鼠進(jìn)行神經(jīng)行為學(xué)評(píng)分,斷頭取腦進(jìn)行TTC染色,檢測(cè)大鼠血清中乳酸脫氫酶(LDH)、肌酸激酶(CK)、谷氨酸(Glu)含量以及腦含水量、腦梗死體積百分比改變。結(jié)果:與模型對(duì)照組相比,莪術(shù)高劑量組LDH、CK、Glu含量以及腦含水量、腦梗死體積百分比有明顯降低(P0.05),莪術(shù)中劑量組LDH、Glu含量和腦梗死體積百分比也有明顯降低(P0.05),而莪術(shù)中劑量組CK、腦含水量和莪術(shù)低劑量組LDH、CK、Glu、腦含水量和腦梗死體積百分比與模型對(duì)照組相比差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。說(shuō)明莪術(shù)在一定劑量下能有效地降低局灶性腦缺血大鼠LDH、CK和Glu含量以及腦含水量、腦梗死體積百分比。結(jié)論:莪術(shù)對(duì)局灶性腦缺血模型大鼠具有一定的神經(jīng)保護(hù)作用,這種作用可能與保護(hù)缺血區(qū)腦組織、減輕腦水腫、抗氧自由基,減少興奮性氨基酸毒性有關(guān)。
[Abstract]:Aim: to investigate the neuroprotective effect and mechanism of Zedoary on focal cerebral ischemia in rats. Methods: sixty adult male SD rats were randomly divided into six groups: sham operation control group, model control group, nimodipine group, zedoary high, middle and low dose groups. In addition to the sham operation control group, the middle cerebral artery obstruction model was made by the method of thread embolization. Prophylactic administration was given for 7 days before modeling, and once a day for 3 days after modeling. The neurobehavioral score and TTC staining were used to detect the contents of lactate dehydrogenase (LDH), creatine kinase (CK), glutamate (Glu), brain water content and the percentage of cerebral infarction volume. Results: compared with the model control group, the content of Glu and the water content of brain in the high dose group of Rhizoma Curcumae were higher than those in the control group. The percentage of cerebral infarction volume decreased significantly (P 0.05), and the content of LDH Glu and the percentage of cerebral infarction volume decreased significantly in the middle dose group of zedoary curcuma, while in the middle dose group of zedoary, the contents of CK, the water content in the brain and LDHK CK Glul in the low dose group of zedoary, the content of water in the brain and the volume of cerebral infarction in the middle dose group were 100. There was no significant difference between the score ratio and the model control group (P 0.05). The results showed that zedoary curcuma could effectively reduce the contents of LDHCK and Glu, the water content of brain and the percentage of cerebral infarction volume in rats with focal cerebral ischemia at a certain dose. Conclusion: zedoary has a certain neuroprotective effect on focal cerebral ischemia rats, which may be related to the protection of ischemic brain tissue, the reduction of brain edema, anti-oxygen free radical, and the reduction of excitatory amino acid toxicity.
【作者單位】： 廣西醫(yī)科大學(xué)藥學(xué)院藥理學(xué)教研室;廣西中醫(yī)藥大學(xué)藥學(xué)院;
【基金】：廣西中醫(yī)藥管理局面上普通資助項(xiàng)目(No.GZPT1205) 廣西壯族自治區(qū)教育廳高�？茖W(xué)技術(shù)研究一般資助項(xiàng)目(No.YB2014174)
【分類號(hào)】：R285.5;R-332
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本文編號(hào)：2045035