本文選題：宮環(huán)出血 + 肝郁血瘀��； 參考：《湖南中醫(yī)藥大學(xué)》2010年碩士論文

【摘要】：目的：本研究在中醫(yī)理論指導(dǎo)下,探討肝郁血瘀型宮環(huán)出血病證動(dòng)物模型的建立方法,并在此基礎(chǔ)上進(jìn)行發(fā)病機(jī)理的研究。 方法：將大鼠隨機(jī)分為4組：空白組、置環(huán)組、模型組、治療組。通過(guò)長(zhǎng)期慢性束縛應(yīng)激結(jié)合孤養(yǎng)建立起應(yīng)激大鼠的肝郁癥模型,觀察分析在模型建立過(guò)程中實(shí)驗(yàn)動(dòng)物的體重、蔗糖水?dāng)z入量以及行為學(xué)指標(biāo)方面的變化,進(jìn)行分析,尋找合適的動(dòng)物模型建立方法。本研究應(yīng)用一種全新的蛋白組學(xué)技術(shù)——抗體芯片技術(shù)測(cè)量動(dòng)物模型子宮組織細(xì)胞因子水平。分析體重、蔗糖攝入量、行為與肝郁癥的相關(guān)關(guān)系,探討細(xì)胞因子在肝郁血瘀型宮環(huán)出血病病理生理機(jī)制中的作用,觀測(cè)治療藥物對(duì)這些指標(biāo)的影響以佐證模型。 結(jié)果：模型組大鼠行為異常,主要表現(xiàn)為躲避、活動(dòng)減少、大便松散、皮色失去光澤,生長(zhǎng)較對(duì)照組緩慢(P0.05)。糖水偏好實(shí)驗(yàn)顯示造模過(guò)程中模型組對(duì)糖水的偏好攝入少于對(duì)照組(P0.05)。與置環(huán)組相比,肝郁血瘀+置環(huán)模型組有4種因子的水平發(fā)生了顯著變化。其中睫狀神經(jīng)營(yíng)養(yǎng)因子(CNTF)水平同置環(huán)組相比降低(P0.05),白介素-1β(IL-1β)、白介素-6(IL-6)、腫瘤壞死因子-α(TNF-α)水平較置環(huán)組顯著升高(P0.05)。 結(jié)論：金刃損傷胞宮是宮環(huán)出血關(guān)鍵病因,肝氣郁結(jié),瘀血阻絡(luò)為其主要發(fā)病機(jī)理。本試驗(yàn)從病因?qū)W、行為學(xué)和病理表現(xiàn)上用慢性束縛的方法成功構(gòu)建了肝郁證大鼠模型。研究提示,抗體芯片技術(shù)能夠高通量、平行性檢測(cè)大鼠子宮多種細(xì)胞因子的表達(dá)水平,CNTF、IL-1β、IL-6、TNF-α可能與肝郁癥發(fā)病機(jī)制有關(guān)�？贵w芯片技術(shù)有可能成為中醫(yī)癥候?qū)W研究的新型技術(shù)平臺(tái)。
[Abstract]:Objective: under the guidance of traditional Chinese medicine theory, this study explored the establishment of animal model of uterine ring hemorrhage syndrome of liver stagnation and blood stasis, and studied the pathogenesis on the basis of it. Methods: rats were randomly divided into 4 groups: blank group, ring setting group, model group and treatment group. The liver depression model of stress rats was established by long-term chronic restraint stress combined with solitary rearing. The changes of body weight, sucrose water intake and behavioral indexes of experimental animals were observed and analyzed during the establishment of the model. To find a suitable method for establishing animal model. In this study, a novel proteomics technique, antibody chip technique, was used to measure cytokine levels in uterine tissues of animal models. The relationship between body weight, sucrose intake, behavior and liver depression was analyzed. The role of cytokines in the pathophysiological mechanism of uterine circulation hemorrhage with liver depression and blood stasis was discussed. The effects of therapeutic drugs on these indexes were observed to prove the model. Results: the rats in the model group showed abnormal behavior, such as avoidance, decreased activity, loose stool, lost luster of skin color and slower growth than that of the control group (P 0.05). Sugar water preference experiment showed that the preference intake of sugar water in model group was less than that in control group (P 0.05). Compared with the ring setting group, there were significant changes in the level of four factors in the model group of liver stagnation and blood stasis. The level of ciliary neurotrophic factor CNTF- 偽 was significantly higher than that of the ring setting group (P 0.05), and the levels of IL 1 尾, IL 1 尾, IL 6 and TNF- 偽 were significantly higher than those in the ring setting group. Conclusion: the damage of the uterus is the key cause of uterine ring hemorrhage, the main pathogenesis is stagnation of liver-qi and obstruction of collaterals by blood stasis. In this study, the rat model of liver depression was successfully established by chronic restraint in etiology, behavior and pathology. It was suggested that the antibody chip technique could be used to detect the expression level of multiple cytokines in rat uterus in parallel mode. The results suggested that IL-6 TNF- 偽 might be related to the pathogenesis of liver depression. Antibody chip technology may become a new technology platform for TCM syndrome research.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R-332;R271.9

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 俞丕超;張建偉;;婦科病證結(jié)合動(dòng)物模型的研究[J];長(zhǎng)春中醫(yī)藥大學(xué)學(xué)報(bào);2012年03期

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本文編號(hào)：2038301