本文選題：腫瘤壞死因子α + NIH3T3細(xì)胞��； 參考：《青島大學(xué)》2010年碩士論文

【摘要】： 目的：探討腫瘤壞死因子-α(TNF-α)及其信號傳導(dǎo)途徑中特異性激酶抑制劑對NIH3T3細(xì)胞成熟化所起的作用。 方法：體外培養(yǎng)NIH3T3成纖維細(xì)胞,將細(xì)胞分為空白對照組(A組)、TNF-α組(B組)及TNF-α+Anti-TNFRSFIB組(C組)。細(xì)胞制成2×105/mL的細(xì)胞懸液后,接種于25cm2培養(yǎng)瓶中,待細(xì)胞呈匯合狀態(tài)時,換用無血清DMEM高糖培養(yǎng)基培養(yǎng)12-16h。然后A組換用含體積分?jǐn)?shù)為0.02胎牛血清的DMEM高糖培養(yǎng)基繼續(xù)培養(yǎng)；B組換用含100μg/L TNF-α的培養(yǎng)基培養(yǎng)；C組先加入濃度為50u g/L的Anti-TNFRSFIB作用于細(xì)胞1h后,倒出培養(yǎng)基后再加入含100μg/L TNF-α的培養(yǎng)基繼續(xù)培養(yǎng)。然后采用RT-PCR法測定各組Ⅰ型膠原和基質(zhì)金屬蛋白酶3 (MMP3) mRNA的表達(dá),Western Blot法測定各組Ⅰ型膠原蛋白和MMP3蛋白的表達(dá)。 結(jié)果：MMP3無論是mRNA還是蛋白的表達(dá)B組和C組與A組相比均有顯著性差異(t=-13.413-5.076,P0.05)。其中B組與C組相比,差異也具有顯著性(t=4.441-5.076,P0.01)；Ⅰ型膠原二者的表達(dá)B組和C組與A組相比也均有顯著性差異(t=-4.950-5.808,P0.05),B組與C組民較,差異顯著(t=-4.950--3.823,P0.05)。 結(jié)論：與對照組相比,經(jīng)TNF-α干預(yù)后,NIH3T3成纖維細(xì)胞中Ⅰ型膠原蛋白表達(dá)明顯減少,而MMP3的表達(dá)量則增多。TNF-α促進了NIH3T3的活化。
[Abstract]:Aim: to investigate the effect of specific kinase inhibitor on the maturation of NIH3T3 cells by tumor necrosis factor- 偽 (TNF- 偽) and its signal transduction pathway. Methods: NIH3T3 fibroblasts were cultured in vitro. The cells were divided into two groups: control group (group A) and TNF- 偽 Anti-TNFRSFIB group (group C). Cells were prepared into 2 脳 105 / mL cell suspensions and inoculated in 25cm2 culture flask. When the cells were confluent, the cells were cultured in serum-free high glucose medium for 12-16 hours. Then group A was treated with DMEM high sugar medium containing 0.02 fetal bovine serum by volume fraction. Group B was transferred to culture medium containing 100 渭 g / L TNF- 偽. Group C was treated with 50 u g / L Anti-TNFRSFIB for 1 h. Culture medium containing 100 渭 g / L TNF- 偽 was added. Then the mRNA expression of collagen 鈪，

本文編號：2031331