本文選題：骨髓間充質(zhì)干細(xì)胞 + 表皮干細(xì)胞��； 參考：《中南大學(xué)》2009年碩士論文

【摘要】： 目的:研究HaCaT細(xì)胞誘導(dǎo)MSCs向表皮干細(xì)胞分化的機(jī)制。 方法:1密度梯度離心法分離、培養(yǎng)MSCs,免疫組織化學(xué)法鑒定CD34,CD44和CD45。2用插入式細(xì)胞培養(yǎng)器將HaCaT和MSCs共培養(yǎng),設(shè)為實驗組;MSCs單獨培養(yǎng),設(shè)為對照組。分別于第3和第7天免疫組織化學(xué)法鑒定CK19,Pan-ck和β1整合素。3試驗分為3組,分別為共培養(yǎng)細(xì)胞上清液組,HaCaT上清液組和MSCs上清液組。ELISA法檢測上清液中EGF含量。 結(jié)果:1分離出的細(xì)胞呈梭形,免疫組織化學(xué)染色CD34,CD45表達(dá)陰性,CD44表達(dá)陽性。2共培養(yǎng)后MSCs免疫組織化學(xué)染色CK19,Pan-ck和β1整合素陽性,共培養(yǎng)7天后MSCs CK19,Pan-ck和β1整合素的陽性率均高于共培養(yǎng)3天的(P＜0.05)。3共培養(yǎng)組上清液中EGF均值高于HaCaT組(P＜0.01)。 結(jié)論:1 HaCaT細(xì)胞可以誘導(dǎo)MSCs向表皮干細(xì)胞分化。2 EGF是HaCaT細(xì)胞誘導(dǎo)MSCs向表皮干細(xì)胞分化的因素之一。
[Abstract]:Objective: to study the mechanism of differentiation of MSCs into epidermal stem cells induced by HaCaT cells. Methods MSCs were isolated and cultured by the density gradient centrifugation method of 1: 1. CD34T CD44 and CD45.2 were identified by immunohistochemical method. HaCaT and MSCs were co-cultured in a plug-in cell culture apparatus. The MSCs were cultured separately in the experimental group and in the control group. On the 3rd and 7th day, CK19 Pan-ck and 尾 1 integrin 3 test were divided into three groups, the supernatant group of co-cultured cells, HaCaT supernatant group and MSCs supernatant group. Elisa was used to detect the content of EGF in supernatant. Results the cells isolated from 1 / 1 were fusiform. The positive expression of CD34, CD45, CD44, CD44, and 尾 1 integrin in MSCs were detected by immunohistochemistry, and the positive expression of CD44 and 尾 1 integrin in MSCs were detected by immunohistochemistry. The positive rates of CK19 Pan-ck and 尾 1 integrin in MSCs co-cultured for 7 days were higher than those in HaCaT group (P < 0.05). Conclusion the differentiation of MSCs into epidermal stem cells induced by 1: 1 HaCaT cells. 2 EGF is one of the factors that induce MSCs to differentiate into epidermal stem cells.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：R329

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