本文選題：單核細(xì)胞增生李斯特菌 + 秀麗隱桿線蟲��； 參考：《微生物學(xué)雜志》2015年04期

【摘要】：單核細(xì)胞增生李斯特菌(Listeria monocytogenes,Lm)是李斯特菌病的病原細(xì)菌。用Lm野生株EGDe、弱毒株Δprf A、毒力回復(fù)株+prf A和高毒株+prf A*喂飼模式生物秀麗隱桿線蟲N2,并以線蟲的良好食源大腸埃希菌OP50以及非致病的無害李斯特菌(Listeria innocua)作為對照,檢測Lm對線蟲發(fā)育周期、壽命和產(chǎn)卵數(shù)的影響。結(jié)果顯示:當(dāng)以無害李斯特菌、Lm野生株以及Prf A突變株為食時,線蟲的產(chǎn)卵數(shù)雖有所下降,但線蟲不僅能夠正常產(chǎn)卵,而且其發(fā)育周期和壽命均較以O(shè)P50為食時顯著延長(P≤0.05);線蟲體表和消化道中均可檢測到大量李斯特菌,但糞便中的活菌數(shù)極少。以上結(jié)果說明Lm不能殺死秀麗隱桿線蟲,對線蟲也沒有顯著致病性,不適合作為研究Lm致病機(jī)制的模型;Lm可在線蟲體表和消化道存在,暗示Lm可借助線蟲在土壤環(huán)境中生存和傳播。
[Abstract]:Listeria monocytogenes (Lm) is the pathogen of Listeria monocytogenes. Lm wild strain EGDe, attenuated strain 螖 prf A, virulence response strain prf A and high virulent strain prf A * were fed with the model organism N2, and the good food source Escherichia coli OP50 and the non-pathogenic harmless Listeria innocua were used as control. The effects of LM on the developmental cycle, longevity and spawning number of nematodes were detected. The results showed that the number of oviposition of nematode decreased, but nematode could not only lay eggs normally, but also the wild strain of Lm and the mutant of Prf A were feeding on it. The developmental cycle and life span were significantly longer than that of OP50, P 鈮，

本文編號：1973388